The KSFM Journal of Fluid Machinery (한국유체기계학회 논문집)

Korean Society for Fluid machinery (한국유체기계학회)
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Geometric Optimization of a Microchannel for the Improvement of Temperature Gradient Focusing

온도기울기 농축(TGF) 향상을 위한 미세채널 형상 최적화 연구

  • Received : 2010.10.25
  • Accepted : 2011.02.17
  • Published : 2011.04.01
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Abstract

Temperature gradient focusing (TGF) of analytes via Joule heating is achieved when electric field is applied along a microchannel of varying width. The effect of varying width of the microchannel for the focusing performance of the device was numerically studied. The governing equations were implemented into a quasi-1D numerical model along a microchannel. The validity of the numerical model was verified by a comparison between numerical and experimental results. The distributions of temperature, velocity, and concentration along a microchannel were predicted by the numerical results. The narrower middle width and wider outside width of the channel having the fixed length contribute to improve the focusing performance of the device. However, too narrow middle width of the channel generates a higher temperature which can cause the problems including sample denaturation and buffer solution boiling. Therefore, the channel geometry should be optimized to prevent these problems. The optimal widths of the microchannel for the improvement on TGF were proposed and this model can be easily applied to lab-on-a-chip (LOC) applications where focusing is required based on its simple design.

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References

  1. Khandurina, J., Jacobson, S. C., Waters, L. C., Foote, R. S. and Ramsey, J. M., 1999, “Microfabricated Porous Membrane Structure for Sample Concentration and Electrophoretic Analysis,” Analytical Chemistry, Vol. 71, No. 9, pp. 1815-1819. https://doi.org/10.1021/ac981161c
  2. Hatch, A. V., Herr, A. E., Throckmorton, D. J., Brennan, J. S. and Singh, A. K., 2006, “Integrated Preconcentration SDS-PAGE of Proteins in Microchips Using Photopatterned Cross-Linked Polyacrylamide Gels,” Analytical Chemistry, Vol. 78, No. 14, pp. 4976-4984. https://doi.org/10.1021/ac0600454
  3. Wang, Y. C., Stevens, A. L. and Han, J. Y., 2005, “Million-fold Preconcentration of Proteins and Peptides by Nanofluidic Filter,” Analytical Chemistry, Vol. 77, No. 14, pp. 4293-4299. https://doi.org/10.1021/ac050321z
  4. Kim, S. M., Burns, M. A. and Hasselbrink, E. F., 2006, “Electrokinetic Protein Preconcentration Using a Simple Glass/Poly(dimethylsiloxane) Microfluidic Chip,” Analytical Chemistry, Vol. 78, No. 14, pp. 4779-4785. https://doi.org/10.1021/ac060031y
  5. Yu, C., Davey, M. H., Svec, F. and Frechet, J. M. J., 2001, “Monolithic Porous Polymer for On-Chip Solid-Phase Extraction and Preconcentration Prepared by Photoinitiated in Situ Polymerization within a Microfluidic Device,” Analytical Chemistry, Vol. 73, No. 21, pp. 5088-5096. https://doi.org/10.1021/ac0106288
  6. Koegler, W. S. and Ivory, C. F., 1996, “Focusing Proteins in an Electric Field Gradient,” Journal of Chromatography A, Vol. 726, No. 1-2, pp. 229-236. https://doi.org/10.1016/0021-9673(95)01069-6
  7. Petsev, D. N., Lopez, G. P., Ivory, C. F. and Sibbett, S. S., 2005, “Microchannel Protein Separation by Electric Field Gradient Focusing,” Lab on a Chip, Vol. 5, No. 6, pp. 587-597. https://doi.org/10.1039/b501538c
  8. Righetti, P. G. and Bossi, A., 1998, “Isoelectric Focusing of Proteins and Peptides in Gel Slabs and in Capillaries,” Analytica Chimica Acta, Vol. 372, No. 1-2, pp. 1-19. https://doi.org/10.1016/S0003-2670(98)00329-8
  9. Gebauer, P. and Bocek, P., 2002, “Recent Progress in Capillary Isotachophoresis,” Electrophoresis, Vol. 23, No. 22-23, pp. 3858-3864. https://doi.org/10.1002/elps.200290006
  10. Ross, D. and Locascio, L. E., 2002, “Microfluidic Temperature Gradient Focusing,” Analytical Chemistry, Vol. 74, No. 11, pp. 2556-2564. https://doi.org/10.1021/ac025528w
  11. Balss, K. M., Ross, D., Begley, H. C., Olsen, K. G. and Tarlov, M. J., 2004, “DNA Hybridization Assays Using Temperature Gradient Focusing and Peptide Nucleic Acids,” Analytical Chemistry, Vol. 126, No. 41, pp. 13474-13479. https://doi.org/10.1021/ja030667w
  12. Balss, K. M., Vreeland, W. N., Phinney, K. W. and Ross, D., 2004, “Simultaneous Concentration and Separation of Enantiomers with Chiral Temperature Gradient Focusing,” Analytical Chemistry, Vol. 76, No. 24, pp. 7243-4249. https://doi.org/10.1021/ac049046r
  13. Balss, K. M., Vreeland, W. N., Howell, P. B., Henry, A. C. and Ross, D., 2004, “Micellar Affinity Gradient Focusing; A New Method for Electrokinetic Focusing,” Journal of the American Chemical Society, Vol. 126, No. 7, pp. 1936-1937. https://doi.org/10.1021/ja0385641
  14. Kim, S. M., Sommer, G. J., Burns, M. A., and Hasselbrink, E. F., 2006, “Low-Power Concentration and Separation Using Temperature Gradient Focusing via Joule Heating,” Analytical Chemistry, Vol. 78, No. 23, pp. 8028-8035. https://doi.org/10.1021/ac061194p
  15. Sommer, G. J., Kim, S. M., Littrell, R. J. and Hasselbrink, E. F., 2007, “Theoretical and Numerical Analysis of Temperature Gradient Focusing via Joule Heating,” Lab on a Chip, Vol. 7, No. 7, pp. 898-907. https://doi.org/10.1039/b701894k
  16. Wooding, R. A., 1960, “Instability of a Viscous Liquid of Variable Density in a Vertical Hele-Shaw Cell,” Journal of Fluid Mechanics, Vol. 7, pp. 501-515. https://doi.org/10.1017/S0022112060000256
  17. Tang, G. Y., Yan, D. G., Yang, C., Gong, H. Q., Chai, J. C. and Lam, Y. C., 2006, “Assessment of Joule Heating and its Effects on Electroosmotic Flow and Electrophoretic Transport of Solutes in Microfluidic Channels,” Electrophoresis, Vol. 27, No. 3, pp. 628-639. https://doi.org/10.1002/elps.200500681
  18. Tang, G. Y., Yang, C., Gong, H. Q., Chai, J. C. and Lam, Y. C., 2006, “Numerical Simulation of Joule Heating Effect on Sample Band Transport in Capillary Electrophoresis,” Analytica Chimica Acta, Vol. 561, No. 1-2, pp. 138-149. https://doi.org/10.1016/j.aca.2005.12.068
  19. Probstein, R. F., 2003, Physicochemical Hydrodynamics, John Wiley and Sons, Inc., New York, USA.
  20. Incropera, F. P. and DeWitt, D. P., 1996, Fundamentals of Heat and Mass Transfer, John Wiley and Sons, Inc., New York, USA.
  21. Allen, M. W., 2007, Thermal Denaturation of DNA in Semimicro Cells, Thermo Fisher Scientific Inc., Madison, WI, USA.