The Journal of Internal Korean Medicine (대한한방내과학회지)

The Society of Internal Korean Medicine (대한한방내과학회)
권호 표지

Effects of Soshiho-tang on Hydrogen Peroxide-induced Oxidative Damage in Hepatocytes

과산화수소로 유도된 산화성 간세포 손상에 대한 소시호탕(小柴胡湯)의 효과

  • Seo, Sang-Hee (Center for Herbal Medicine Improvement Research, Korea Institute of Oriental Medicine (KIOM)) ;
  • Oh, Su-Young (Center for Herbal Medicine Improvement Research, Korea Institute of Oriental Medicine (KIOM)) ;
  • Lee, Ji-Seon (Center for Herbal Medicine Improvement Research, Korea Institute of Oriental Medicine (KIOM)) ;
  • Cho, Won-Kyung (Center for Herbal Medicine Improvement Research, Korea Institute of Oriental Medicine (KIOM)) ;
  • Kim, Tae-Soo (Center for Herbal Medicine Improvement Research, Korea Institute of Oriental Medicine (KIOM)) ;
  • Ma, Jin-Yeul (Center for Herbal Medicine Improvement Research, Korea Institute of Oriental Medicine (KIOM))
  • 서상희 (한국한의학연구원 신한방제제연구센터) ;
  • 오수영 (한국한의학연구원 신한방제제연구센터) ;
  • 이지선 (한국한의학연구원 신한방제제연구센터) ;
  • 조원경 (한국한의학연구원 신한방제제연구센터) ;
  • 김태수 (한국한의학연구원 신한방제제연구센터) ;
  • 마진열 (한국한의학연구원 신한방제제연구센터)
  • Published : 2011.12.30
Download PDF
⟨ Previous Next ⟩

Abstract

Objectives : The aim of this study was to investigate the hepatoprotective effect of Soshiho-tang (SSH) in mouse primary liver cells against hydrogen peroxide ($H_2O_2$)-induced oxidative stress. We also elucidated the molecular mechanism of hepatoprotective effect by SSH. Methods : Cell viability, level of ALT, AST and LDH, intracellular ROS level, mRNA expression and activity of antioxidant enzymes were used to evaluate hepatoprotection of SSH against $H_2O_2$. Target gene expressions were analyzed by real-time PCR. Results : Pre-treatment with SSH for 1 hour prevented cytotoxicity against $H_2O_2$. $H_2O_2$-induced ROS level decreased under SSH pre-treatment. mRNA expression of GPx and SOD increased in SSH-treated cells. In addition, HSP72 and HSP40 gene expression were elevated under SSH-treatment. Conclusions : These results indicate that SSH protects mouse primary liver cells from $H_2O_2$-induced oxidative injury. This hepatoprotective activity of SSH is mediated by decreasing intracellular ROS and increasing antioxidant enzyme expression (GPx and SOD) and stress response protein (HSP72 and HSP40).

Keywords

References

  1. Hirayama C, Okumura M, Tanikawa K, Yano M, Mizuta M, Ogawa N. A multicenter randomized controlled clinical trial of Shosaiko-to in chronic active hepatitis. Gastroenterol Jpn 1989;24(6):715-9.
  2. Tajiri H, Kozaiwa K, Ozaki Y, Miki K, Shimuzu K, Okada S. Effect of sho-saiko-to(xiao-chaihu-tang) on HBeAg clearance in children with chronic hepatitis B virus infection and with sustained liver disease. Am J Chin Med 1991;19(2):121-9. https://doi.org/10.1142/S0192415X91000193
  3. Oka H, Yamamoto S, Kuroki T, Harihara S, Marumo T, Kim SR, et al. Prospective study of chemoprevention of hepatocellular carcinoma with Sho-saiko-to(TJ-9). Cancer 1995;76(5):743-9. https://doi.org/10.1002/1097-0142(19950901)76:5<743::AID-CNCR2820760506>3.0.CO;2-V
  4. Amagaya S, Umeda M, Ogihara Y. Inhibitory action of shosaikoto and daisaikoto(traditional Chinese medicine) on collagen-induced platelet aggregation and prostaglandin biosynthesis. Planta Med 1986;5:345-9.
  5. Sakaguchi S, Tsutsumi E, Yokota K. Preventive effects of a traditional Chinese medicine(shosaiko-to) against oxygen toxicity and membrane damage during endotoxemia. Biol Pharm Bull 1993;16:782-6. https://doi.org/10.1248/bpb.16.782
  6. Fujiwara K, Mochida S, Nagoshi S. Regulation of hepatic macrophage function by oral administration of xiao-chai-hu-tang(sho-saiko-to, TJ-9) in rats. J Ethnopharmacol 1995;46:107-14. https://doi.org/10.1016/0378-8741(95)01235-6
  7. Amagaya S, Hayagawa M, Ogihara Y, Fujiwara K. Effect of Syo-saiko-to and Dai-saiko-to on carbon tetrachlorideinduced hepatic injury in rats. J Med Pharm Soc Wakan Yaku 1988;5:129-36.
  8. Amagaya S, Miyake A, Ogihara Y, Fujiwara K. Effect of Syo-saiko-to on liver regeneration in partially hepatectomized rats. J Med Pharm Soc Wakan Yaku 1988;5:137-45.
  9. Haranaka K, Satomi N, Sakurai A et al. Antitumor activities and tumor necrosis factor producibility of traditional Chinese medicines and crude drugs. Cancer Immunol Immunother 1985;20:1-5.
  10. Ito H, Shimura K. Effects of a blended Chinese medicine, Xiao-ChaiHu-Tang, on Lewis lung carcinoma growth and inhibition of lung metastasis, with special reference to macrophage activation. Jpn J Pharmacol 1986;41:307-14. https://doi.org/10.1254/jjp.41.307
  11. 권영모, 이재은, 박선동, 시호가 acetaminophen으로 유도된 간손상 흰쥐에 미치는 영향. 대한본초학회지 2004;19(4):91-104.
  12. 김대근, 김만배, 김훈, 박진한, 임종필, 홍승헌. 본초생약학 4. 서울: 도서출판 신일북스; 2009, p. 104-6, 358-61, 348-51, 287-8, 55-7, 352-3.
  13. Cesaratto L, Vascotto C, Calligaris S, Tell G. The importance of redos state in liver damage. Ann Hepatol 2004;3(3):86-92.
  14. Ma SF, Nishikawa M, Katsumi H, Yamashita F, Hashida M. Liver targeting of catalase by cationization for prevention of acute liver failure in mice. J Control Release 2006;110(2):273-82. https://doi.org/10.1016/j.jconrel.2005.10.016
  15. Dang SS, Wang BF, Cheng YA, Song P, Liu ZG, Li ZF. Inhibitory effects of saikosaponin-d on CCl4-induced hepatic fibrogenesis in rats. World J Gastroenterol 2007;13(4):557-63.
  16. 안영근, 김성오, 정대영. 랏트에 있어서 사염화탄소 독성에 대한 소시호탕 엑기스와 prednisolone의 영향. 환경독성보건학회 1989;4(2):61-75.
  17. Taira Z, Yabe K, Hamaguchi Y, Hirayama K, Kishimoto M, Ishida S, et al. Effects of Shosaiko-to extract and its components, Baicalin, baicalein, glycyrrhizin and glycyrrhetic acid, on pharmacokinetic behavior of salicylamide in carbon tetrachloride intoxicated rats. Food Chem Toxicol 2004;42(5):803-7. https://doi.org/10.1016/j.fct.2003.12.017
  18. Berry MN, Freind DS. High-yield preparation of isolated rat liver parenchymal. A biochemical and fine structural study. J Cell Biol 1969;43:506-20. https://doi.org/10.1083/jcb.43.3.506
  19. Moseley PL. Heat shock proteins and the inflammatory response. Ann N Acad Sci 1998;856:206-13. https://doi.org/10.1111/j.1749-6632.1998.tb08327.x
  20. Febbraio MA, Koukoulas I. HSP72 gene expression progressively increases in human skeletal muscle during prolonged, exhaustive exercise. J Appl Physiol 2000;89(3):1055-60.
  21. 이종은. 스트레스단백질과허혈성내성. J Neurocrit Care 2008;1:12-21.
  22. Van Meerloo J, Kaspers GJ, Cloos J. Cell sensitivity assays: the MTT assay. Methods Mol Biol 2011;731:237-45.
  23. Koh JY, Choi DW. Quantitative determination of glutamate mediated cortical neuronal injury in cell culture by lactate dehydrogenase efflux assay. J Neurosci Methods 1987;20:83-90. https://doi.org/10.1016/0165-0270(87)90041-0
  24. 박영숙. 고지방 식이 흰쥐에서 오가피 추출물의 항산화 효과 및 지질 개선 효과. 동아시아식생활학회지 2010;20(1):37-45.
  25. Vega VL, Charles W, De Maio A. A new feature of the stress response: increase in endocytosis mediated by Hsp70. Cell Stress Chaperones 2010;15(5):517-27. https://doi.org/10.1007/s12192-009-0165-2
  26. Sterrenberg JN, Blatch GL, Edkins AL. Human DNAJ in cancer and stem cells. Cancer Lett 2011;312(2):129-42. https://doi.org/10.1016/j.canlet.2011.08.019
  27. 윤길숙. 신세포암종에서 Heat Shock Protein 27의 발현과 세포사멸사에 대한 연구. 대한 병리학회지 2006;40:39-45.