Korean Journal of Medicinal Crop Science (한국약용작물학회지)

The Korean Society of Medicinal Crop Science (한국약용작물학회)
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Antioxidant Activity of Different Solvent Fractions from Prunella vulgaris var. lilacina

꿀풀 에탄올 추출물 및 분획물의 항산화성

  • 박동식 (농촌진흥청 국립농업과학원 농식품자원부) ;
  • 박미영 (농촌진흥청 국립농업과학원 농식품자원부) ;
  • 전상민 (농촌진흥청 국립농업과학원 농식품자원부) ;
  • 이진영 (농촌진흥청 국립농업과학원 농식품자원부) ;
  • 이영민 (농촌진흥청 국립농업과학원 농식품자원부) ;
  • 장환희 (농촌진흥청 국립농업과학원 농식품자원부) ;
  • 황경아 (농촌진흥청 국립농업과학원 농식품자원부) ;
  • 김재현 (농촌진흥청 국립농업과학원 농식품자원부)
  • Received : 2011.10.10
  • Accepted : 2011.12.19
  • Published : 2011.12.30
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Abstract

The potential antioxidant activities of different fractions from Prunella vulgaris var. lilacina were assayed in vitro. Among several fractions, n-BuOH fraction showed the highest 1,1-di[henyl-2-picrylhydrazyl (DPPH) free radical scavenging ($IC_{50}=0.50{\mu}g/mL$). The results of 2,2'-azinobis (3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS) radical scavenging activity and ferric reducing antioxidant power (FRAP) assay showed the concentration dependency and n-BuOH fraction appeared a better result than the other fractions at the same concentrati on in this study. Moreover the total phenol and flavonoid contents of n-BuOH fraction contained the highest level. Additionally, correlation analysis indicated a high correlation between the antiradical activity and the total phenolic and flavonoid contents (p < 0.001). It suggests that n-BuOH fraction obtained from the 70% EtOH crude extract of Prunella vulgaris var. lilacina has wide potential for use as a source of antioxidant material.

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References

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