Korean Journal of Medicinal Crop Science (한국약용작물학회지)

The Korean Society of Medicinal Crop Science (한국약용작물학회)
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Effect of Solubility of Thiamine Dilauryl Sulfate Solution through the Manufacture of the Nano Paticles on Antifungal Activity

비타민 B1 유도체 Thiamine Dilauryl Sulfate의 나노 입자 제조를 통한 수용액의 용해도에 따른 항진균 활성 평가

  • 서용창 (의료바이오신소재융복합연구센터 강원대학교 생물소재공학과) ;
  • 최운용 (강원대학교 생물소재공학과) ;
  • 이춘근 (강원대학교 생물소재공학과) ;
  • 조정섭 (두산에코비즈넷) ;
  • 임태빈 (두산에코비즈넷) ;
  • 정명훈 (두산에코비즈넷) ;
  • 김성일 (강원도 농업기술원) ;
  • 윤원병 (강원대학교 식품생명공학과) ;
  • 이현용 (강원대학교 생물소재공학과)
  • Received : 2011.08.23
  • Accepted : 2011.12.07
  • Published : 2011.12.30
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Abstract

Conventional Thiamine Dilauryl Sulfate (TDS) powder has a low stability. In order to solve this problem, this study was performed to improve the solubility of TDS. The process for enhance solubility of TDS was nano grinding mill and ultrasonic dispersion process. TDS paticle was manufactured to nano size through nano grinding mill process. The size of TDS nanoparticle was measured as average 220 nm by DLS. And The TDS nanoparticle in water solution manufactured through ultrasonic dispersion process. The TDS nanoparticle in water solution was showed the highest solubility with 40% ethanol. These results was increased the concentration of TDS from 200 ppm to 240 ppm in water solution. The TDS nanoparticle in water solution showed diameter of Colletotrichum gloeosporioides growth with smaller than about 1.56 cm compared to the TDS paticle in water solution at same concentration. Also, TDS nanoparticle in water solution showed growth inhibition activity as 59.2% with higher than about 10% compared to the TDS paticle water solution in same concentration. Finally, TDS nanoparticle in water solution was increased solubility through nano grinding mill and ultrasonic dispersion process. Also, the increase of concentration in TDS nanopaticle in water solution according to solubility enhancement lead to an result enhancement of antifungal activity. Consequently, we suggested that the TDS nanoparticle in water solution was more effective than TDS particle in water solution owing to the sub-cellular particle size, ability to persistence and targeting to cell membrane of Colletotrichum gloeosporioides. Furthermore we expected the applicating possibility with bio pesticide.

Keywords

References

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