Korean Journal of Medicinal Crop Science (한국약용작물학회지)

The Korean Society of Medicinal Crop Science (한국약용작물학회)
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Cytotoxicity and Antioxidative Activity of Artemisiae Argi Folium Alcoholic Extracts and Their Fractions

애엽(艾葉) 에탄올 및 메탄올 추출물과 용매별 분획의 세포독성과 항산화활성

  • Lee, Kyoung-In (Biotechnology Industrialization Center, Dongshin University) ;
  • Cho, Joo-Hyun (Deptartment of Oriental Medicine Materials, Dongshin University) ;
  • Pyo, Byoung-Sik (Deptartment of Oriental Medicine Materials, Dongshin University)
  • 이경인 (동신대학교 생물자원산업화지원센터) ;
  • 조주현 (동신대학교 한약재산업학과) ;
  • 표병식 (동신대학교 한약재산업학과)
  • Received : 2011.10.13
  • Accepted : 2011.11.30
  • Published : 2011.12.30
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Abstract

This experiment was carried out to obtain the cytotoxicity and antioxidative activity of Artemisiae Argi Folium. The total polyphenol contents in the ethyl acetate fraction of the ethanol extract and the methanol extract were 430.27mg/g and 427.84mg/g, respectively. In DPPH radical scavenging ability, $SC_{50}$ values of the ethyl acetate fraction of the ethanol extract and the methanol extract were 32.64 ${\mu}g/m{\ell}$ and 27.70 ${\mu}g/m{\ell}$ as the same level of statistical with ascorbic acid. In the cytotoxicity measurement by MTT assay, the chloroform and hexane fraction, and each extract were exhibited higher cytotoxicity than the other fractions. In particular, the ethyl acetate fractions appeared high activity in DPPH radical scavenging ability were began to show cytotoxicity in 125 ${\mu}g/m{\ell}$. As a result, the ethyl acetate fraction of Artemisiae Argi Folium extract was the most highly active fraction in antioxidative activity. However, for the use of extracts and fractions from Artemisiae Argi Folium to related fields, the setting of appropriate concentration is required.

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