한국식품과학회지 (Korean Journal of Food Science and Technology)

한국식품과학회 (Korean Society of Food Science and Technology)
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살모넬라와 황색포도상구균에 대한 시트랄의 항균효과

Antimicrobial Activity of Citral against Salmonella Typhimurium and Staphylococcus aureus

  • 김정지 (국민대학교 식품영양학과) ;
  • 인예원 (국민대학교 식품영양학과) ;
  • 오세욱 (국민대학교 식품영양학과)
  • Kim, Jung-Jee (Department of Food and Nutrition, Kookmin University) ;
  • In, Ye-Won (Department of Food and Nutrition, Kookmin University) ;
  • Oh, Se-Wook (Department of Food and Nutrition, Kookmin University)
  • 투고 : 2011.07.22
  • 심사 : 2011.09.23
  • 발행 : 2011.12.31
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초록

본 연구는 그람 양성균과 음성균인 S. aureus와 S. Typhimurium를 대상으로 시트랄의 농도와 pH에 따른 항균활성 특성 및 식품 적용 가능성을 조사하였다. 농도별 항균활성은 TSB에 시트랄을 0-1,000 ppm 농도로 처리하였으며, pH는 buffer를 이용하여 pH 4.0에서 pH 7.0로 조정하여 측정하였다. 시트랄 농도 별 항균활성 실험에서는 두 균주 모두 시트랄 농도가 높아질수록 항균활성이 증가하였다. S. Typhimurium는 250 ppm 처리구에서 대조구와 유의적 차이가 없었으나 S. aureus는 약 3 log 수준으로 감소되었다. pH에 따른 항균활성은 10분이 경과 후 S. aureus는 약 1-2 log의 감소효과를, S. Typhimurium는 약 2-5 log의 감소효과를 나타내었다. TSB와는 다르게 buffer 조건에서 그람 양성 균주인 S. aureus는 S. Typhimurium와 비교하여 pH 조건에 안정적인 경향을 나타내었다. 녹즙에 적용한 시트랄은 그람 음성균인 S. Typhimurium에 대한 항균활성은 미미하였으나 양성균인 S. aureus 에 대하여 농도가 증가할수록 효과적으로 항균활성이 증가하였다.

The aim of this study was to investigate the antimicrobial characteristics of citral against Salmonella Typhimurium and Staphylococcus aureus. Antimicrobial activities were determined according to the citral concentration and initial pH. The tested citral concentrations were 0-1,000 ppm in tryptic soy broth (TSB) and 0-5,000 ppm in Angelica keiskei juice (NokJeup). The initial pHs tested were 4-7. Antimicrobial activities increased as citral concentration increased. S. aureus was more susceptible than S. Typhimurium during culture in TSB. But S. aureus was less susceptible to pH changes. Citral caused about 1-2 log reduction of S. aureus and 2-5 log reduction of S. Typhimurium after 10 min exposure at different pHs. As the citral concentration in the Algelica keiskei juice increased, S. aureus was easily inactivated but S. Typhimurium was not inactivated.

키워드

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피인용 문헌

  1. Citral, a monoterpenoid aldehyde interacts synergistically with norfloxacin against methicillin resistant Staphylococcus aureus vol.34, 2017, https://doi.org/10.1016/j.phymed.2017.08.016
  2. Antimicrobial activities of actinonin against Bacillus cereus vol.48, pp.6, 2016, https://doi.org/10.9721/KJFST.2016.48.6.560