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Korean Society for Biochemistry and Molecular Biology (생화학분자생물학회)
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EphrinB1 interacts with the transcriptional co-repressor Groucho/xTLE4

  • Kamata, Teddy (Laboratory of Cell and Developmental Signaling, National Cancer Institute-Frederick) ;
  • Bong, Yong-Sik (Laboratory of Cell and Developmental Signaling, National Cancer Institute-Frederick) ;
  • Mood, Kathleen (Laboratory of Cell and Developmental Signaling, National Cancer Institute-Frederick) ;
  • Park, Mae-Ja (Department of Anatomy, School of Medicine, Kyungpook National University) ;
  • Nishanian, Tagvor G. (Laboratory of Cell and Developmental Signaling, National Cancer Institute-Frederick) ;
  • Lee, Hyun-Shik (Laboratory of Cell and Developmental Signaling, National Cancer Institute-Frederick)
  • Received : 2010.11.29
  • Accepted : 2010.12.23
  • Published : 2011.03.31
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Abstract

Ephrin signaling is involved in various morphogenetic events, such as axon guidance, hindbrain segmentation, and angiogenesis. We conducted a yeast two-hybrid screen using the intracellular domain (ICD) of EphrinB1 to gain biochemical insight into the function of the EphrinB1 ICD. We identified the transcriptional co-repressor xTLE1/Groucho as an EphrinB1 interacting protein. Whole-mount in situ hybridization of Xenopus embryos confirmed the co-localization of EphrinB1 and a Xenopus counterpart to TLE1, xTLE4, during various stages of development. The EphrinB1/xTLE4 interaction was confirmed by co-immunoprecipitation experiments. Further characterization of the interaction revealed that the carboxy-terminal PDZ binding motif of EphrinB1 and the SP domain of xTLE4 are required for binding. Additionally, phosphorylation of EphrinB1 by a constitutively activated fibroblast growth factor receptor resulted in loss of the interaction, suggesting that the interaction is modulated by tyrosine phosphorylation of the EphrinB1 ICD.

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References

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