The Plant Pathology Journal

한국식물병리학회 (The Korean Society of Plant Pathology)
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Screening of Rhizobacteria for Biological Control of Cucumber Root and Crown Rot Caused by Phytophthora drechsleri

  • Maleki, Mojdeh (Department of plant protection, Islamic Azad University of Varamin-Pishva) ;
  • Mokhtarnejad, Lachin (Department of plant protection, University of Tehran) ;
  • Mostafaee, Somayyeh (Department of plant protection, Islamic Azad University of Varamin-Pishva)
  • 투고 : 2010.11.05
  • 심사 : 2011.02.12
  • 발행 : 2011.03.01
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초록

Antagonistic rhizobacteria, more specifically fluorescent pseudomonads and certain species of Bacillus, are known as biocontrol agents of fungal root diseases of agronomic crops. In this study, 144 bacteria were isolated from cucumber rhizosphere and screened as potential biological control agents against Phytophthora drechsleri, the causal agent of cucumber root rot, in vitro condition. Non-volatile compounds of 23 isolates showed noticeable inhibition zone (> 30%) against P. drechsleri, whereas volatile compounds of 7 isolates could prevent more than 30% of the mycelial growth of the fungus. All promising isolates, except of Pseudomonas flourescens V69, promoted significantly plant growth under in vitro condition. P. flourescens CV69 and V11 exhibited the highest colonization on the root. Results of the greenhouse studies showed that a reduction in disease incidence by use of some strains, and particularly use of strains CV6 and V11 as a soil treatment, exhibited a reduction in disease incidence so that suppressed disease by 85.71 and 69.39% respectively. Pseudomonas flourescens CV6 significantly suppressed disease in comparison to Ridomil fungicide. The use of mixture bacterial strains in the soil inoculated by the fungus resulting in falling down the most of the plants which didn't show significant difference with infected control soils without bacteria.

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참고문헌

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