Korean Journal of Environmental Biology (환경생물)

Korean Society of Environmental Biology (한국환경생물학회)
권호 표지

Species Diversity of Betaproteobacteria in the Sumunmulbengdui Wetland Area of Jeju Island and Distribution of Novel Taxa

제주도 숨은물벵뒤 습지 서식 Betaproteobacteria의 종다양성 및 신분류군 분포

  • Shin, Young-Min (Department of Microbiology & Molecular Biology, Chungnam National University) ;
  • Kim, Tae-Ui (Department of Microbiology & Molecular Biology, Chungnam National University) ;
  • Choi, Ah-Young (Department of Biological Sciences, Inha University) ;
  • Chun, Jee-Sun (Department of Biological Sciences, Chonbuk National Universty) ;
  • Lee, Sang-Hoon (Department of Biology, Gangneung-Wonju National University) ;
  • Kim, Ha-Neul (Department of Bioscience and Biotechnology, Hankuk University of Foreign Studies) ;
  • Yi, Ha-Na (School of Biological Sciences, Seoul National University) ;
  • Jo, Jae-Hyung (Department of Bioscience and Biotechnology, Hankuk University of Foreign Studies) ;
  • Cho, Jang-Cheon (Department of Biological Sciences, Inha University) ;
  • Jahng, Kwang-Yeop (Department of Biological Sciences, Chonbuk National Universty) ;
  • Kim, Kyu-Joong (Department of Biology, Gangneung-Wonju National University) ;
  • Joh, Ki-Seong (Department of Bioscience and Biotechnology, Hankuk University of Foreign Studies) ;
  • Chun, Jong-Sik (School of Biological Sciences, Seoul National University) ;
  • Lee, Hyune-Hwan (Department of Bioscience and Biotechnology, Hankuk University of Foreign Studies) ;
  • Kim, Seung-Bum (Department of Microbiology & Molecular Biology, Chungnam National University)
  • 신영민 (충남대학교 생명시스템과학대학 미생물분자생명과학과) ;
  • 김태의 (충남대학교 생명시스템과학대학 미생물분자생명과학과) ;
  • 최아영 (인하대학교 자연과학대학 생명과학과) ;
  • 전지선 (전북대학교 자연과학대학 생명과학과) ;
  • 이상훈 (강릉원주대학교 자연과학대학 생물학과) ;
  • 김하늘 (한국외국어대학교 자연과학대학 생명공학과) ;
  • 이하나 (서울대학교 자연과학대학 생명과학부) ;
  • 조재형 (한국외국어대학교 자연과학대학 생명공학과) ;
  • 조장천 (인하대학교 자연과학대학 생명과학과) ;
  • 장광엽 (전북대학교 자연과학대학 생명과학과) ;
  • 김규중 (강릉원주대학교 자연과학대학 생물학과) ;
  • 조기성 (한국외국어대학교 자연과학대학 생명공학과) ;
  • 천종식 (서울대학교 자연과학대학 생명과학부) ;
  • 이현환 (한국외국어대학교 자연과학대학 생명공학과) ;
  • 김승범 (충남대학교 생명시스템과학대학 미생물분자생명과학과)
  • Received : 2011.05.18
  • Accepted : 2011.07.21
  • Published : 2011.08.31
Download PDF
⟨ Previous Next ⟩

Abstract

The species diversity of Betaproteobacteria in the Sumunmulbengdui Wetland Area of Jeju Island was studied using culture based techniques, and candidates for novel taxa were screened. Twenty two novel bacterial strains belonging to Betaproteobacteria were isolated, which could be assigned to 16 genera of 4 families, namely Burkholderiaceae (3 strains), Comamonadaceae (8 strains), Oxalobacteraceae (5 strains), Neisseriaceae (5 strains), and an unassigned group belonging to Burkholderiales (1 strain) based 16S rRNA gene sequences. The genus Chromobacterium contained three candidates of novel species, and each of the genera Burkholderia, Comamonas, Pelomonas and Herbaspirillum contained two candidates respectively. Through the analysis of membrane fatty acid profiles and physiological properties using API 20NE as well as morphological and cultural properties, each of the isolates was found to form potentially novel species. Brief description of 22 potential candidates for new species or subspecies is given accordingly.

제주도 숨은물벵뒤 습지에서 Betaproteobacteria의 종다양성을 조사하였고, 신분류군 후보 22균주를 확보하였다. 분리주의 16S rRNA 유전자 염기서열을 분석한 결과Burkholderiaceae (3균주), Comamonadaceae (8균주), Oxalobacteraceae (5균주) 및 Neisseriaceae (5균주) 등 4개 과에 속한 15속, 그리고 소속 미상의 Burkholderiales 소속1속(1균주)으로 동정되었다. Chromobacterium 속에 속한 3종의 신분류군 후보를 확보하였고, Burkholderia, Comamonas, Pelomonas 및 Herbaspirillum의 4속에는 각각 2종씩, 그리고 나머지 속은 각각 1종씩의 신분류군 후보를 확보할 수 있었다. 이들 신분류군 후보에 대해 형태 및 배양학적 특징의 분석과 더불어 막지방산 및 API 20NE 분석을 실시하여 22종의 신분류군 후보에 대한 특징을 기술하였다.

Keywords

References

  1. Aguirre de Carcer D, M Martin, U Karlson and R Rivilla. 2007. Changes in bacterial populations and in biphenyl dioxygenase gene diversity in a polychlorinated biphenyl-polluted soil after introduction of willow trees for rhizoremediation. Appl. Environ. Microbiol. 73:6224-6232. https://doi.org/10.1128/AEM.01254-07
  2. Baldani JI, VLD Baldani, L Seldin and J Dobereiner. 1986. Characterization of Herbaspirillum seropedicae gen. nov., sp. nov., a root-associated nitrogen-fixing bacterium. Int. J. Syst. Bacteriol. 36:86-93. https://doi.org/10.1099/00207713-36-1-86
  3. Callaghan AV, M Tierney, CD Phelps and LY Young. 2009. Anaerobic biodegradation of n-hexadecane by a nitrate-reducing consortium. Appl. Environ. Microbiol. 75:1339-1344. https://doi.org/10.1128/AEM.02491-08
  4. Chun J, JH Lee, Y Jung, M Kim, S Kim, BK Kim and YW Lim. 2007. EzTaxon: a web-based tool for the identification of prokaryotes based on 16S ribosomal RNA gene sequences. Int. J. Syst. Evol. Microbiol. 57:2259-2261. https://doi.org/10.1099/ijs.0.64915-0
  5. Euzeby JP. 2011. List of Prokaryotic Names with Standing in Nomenclature, as of April 2011 (www.bacterio.net).
  6. Garrity GM, JA Bell and T Lilburn. 2005. Class II. Betaproteobacteria class. nov. p. 575. In Bergey's Manual of Systematic Bacteriology, second edition, vol. 2, part C (Brenner DJ, NR Krieg, JT Staley and GM Garrity eds.). Springer. New York.
  7. Gomila M, B Bowien, E Falsen, ERB Moore and J Lalucat. 2007. Description of Pelomonas aquatica sp. nov. and Pelomonas puraquae sp. nov., isolated from industrial and haemodialysis water. Int. J. Syst. Evol. Microbiol. 57:2629-2635. https://doi.org/10.1099/ijs.0.65149-0
  8. Gruwell ME, NB Hardy, PJ Gullan and K Dittmar. 2010. Evolutionary relationships among primary endosymbionts of the mealybug subfamily Phenacoccinae (Hemiptera: Coccoidea: Pseudococcidae). Appl. Environ. Microbiol. 76:7521-7525. https://doi.org/10.1128/AEM.01354-10
  9. Heinzel E, S Hedrich, E Janneck, F Glombitza, J Seifert and M Schlömann. 2009. Bacterial diversity in a mine water treatment plant. Appl. Environ. Microbiol. 75:858-861. https://doi.org/10.1128/AEM.01045-08
  10. Jung SY, MH Lee, TK Oh and JH Yoon. 2007. Herbaspirillum rhizosphaerae sp. nov., isolated from rhizosphere soil of Allium victorialis var. platyphyllum. Int. J. Syst. Evol. Microbiol. 57:2284-2288. https://doi.org/10.1099/ijs.0.64666-0
  11. Kampfer P, HJ Busse and HC Scholz. 2009. Chromobacterium piscinae sp. nov. and Chromobacterium pseudoviolaceum sp. nov., from environmental samples. Int. J. Syst. Evol. Microbiol. 59:2486-2490. https://doi.org/10.1099/ijs.0.008888-0
  12. Larkin MA, G Blackshields, NP Brown, R Chenna, PA McGettigan, H McWilliam, F Valentin, IM Wallace, A Wilm, R Lopez, JD Thompson, TJ Gibson and DG Higgins. 2007. Clustal W and Clustal X version 2.0. Bioinformatics 23:2947-2948. https://doi.org/10.1093/bioinformatics/btm404
  13. Lund MB, M Holmstrup, BA Lomstein, C Damgaard and A Schramm. 2010. Beneficial effect of Verminephrobacter nephridial symbionts on the fitness of the earthworm Aporrectodea tuberculata. Appl. Environ. Microbiol. 76:4738-4743. https://doi.org/10.1128/AEM.00108-10
  14. Martin PAW, D Gundersen-Rindal, M Blackburn and J Buyer. 2007. Chromobacterium subtsugae sp. nov., a betaproteobacterium toxic to Colorado potato beetle and other insect pests. Int. J. Syst. Evol. Microbiol. 57:993-999. https://doi.org/10.1099/ijs.0.64611-0
  15. Narayan KD, SK Pandey and SK Das. 2010. Characterization of Comamonas thiooxidans sp. nov., and comparison of thiosulfate oxidation with Comamonas testosteroni and Comamonas composti. Curr. Microbiol. 61:248-253. https://doi.org/10.1007/s00284-010-9602-9
  16. Park MS, SR Jung, MS Lee, KO Kim, JO Do, KH Lee, SB Kim and KS Bae. 2005. Isolation and characterization of bacteria associated with two sand dune plant species, Calystegia soldanella and Elymus mollis. J. Microbiol. 43:219-227.
  17. Sessitsch A, T Coenye, AV Sturz, P Vandamme, E Ait Barka, JF Salles, JD Van Elsas, D Faure, B Reiter, BR Glick, G Wang-Pruski and J Nowak. 2005. Burkholderia phytofirmans sp. nov., a novel plant-associated bacterium with plantbeneficial properties. Int. J. Syst. Evol. Microbiol. 55:1187-1192. https://doi.org/10.1099/ijs.0.63149-0
  18. Vanlaere E, JJ LiPuma, A Baldwin, D Henry, E De Brandt, E Mahenthiralingam, D Speert, C Dowson and P Vandamme. 2008. Burkholderia latens sp. nov., Burkholderia diffusa sp. nov., Burkholderia arboris sp. nov., Burkholderia seminalis sp. nov. and Burkholderia metallica sp. nov., novel species within the Burkholderia cepacia complex. Int. J. Syst. Evol. Microbiol. 58:1580-1590. https://doi.org/10.1099/ijs.0.65634-0
  19. Weon HY, BY Kim, SH Yoo, JH Joa, SW Kwon and WG Kim. 2007. Andreprevotia chitinilytica gen. nov., sp. nov., isolated from forest soil from Halla Mountain, Jeju Island, Korea. Int. J. Syst. Evol. Microbiol. 57:1572-1575. https://doi.org/10.1099/ijs.0.64694-0
  20. Weon HY, SH Yoo, SB Hong, SW Kwon, E Stackebrandt, SJ Go and BS Koo. 2008. Polaromonas jejuensis sp. nov., isolated from soil in Korea. Int. J. Syst. Evol. Microbiol. 58:1525-1528. https://doi.org/10.1099/ijs.0.65529-0
  21. Weon HY, SH Yoo, SJ Kim, YS Kim, R Anandham and SW Kwon. 2010. Massilia jejuensis sp. nov. and Naxibacter suwonensis sp. nov., isolated from air samples. Int. J. Syst. Evol. Microbiol. 60:1938-1943. https://doi.org/10.1099/ijs.0.015479-0