Novel Taxa Belonging to the Class Alphaproteobacteria, and Gammaproteobacteria, Isolated from the Sumunmulbengdui Wetland Area of Jeju Island

제주도 숨은물벵뒤 습지 서식 Alphaproteobacteria 및 Gammaproteobacteria 강에 속하는 신변이주의 특성

  • Kim, Ha-Neul (Department of Bioscience and Biotechnology, Hankuk University of Foreign Studies) ;
  • Kang, Ji-Young (Department of Biological Sciences, Chonbuk National Universty) ;
  • Choi, Jae-Hee (Ddepartment of Biological Sciences, Inha University) ;
  • Choe, Jeong-Uk (Department of Bioscience and Biotechnology, Hankuk University of Foreign Studies) ;
  • Lee, Sang-Hoon (Department of Biology, Gangneung-Wonju National University) ;
  • Kim, Tae-Ui (Department of Microbiology & Molecular Biology, Chungnam National University) ;
  • Yi, Ha-Na (School of Biological Sciences, Seoul National University) ;
  • Jahng, Kwang-Yeop (Department of Biological Sciences, Chonbuk National Universty) ;
  • Cho, Jang-Cheon (Department of Biological Sciences, Inha University) ;
  • Lee, Hyune-Hwan (Department of Bioscience and Biotechnology, Hankuk University of Foreign Studies) ;
  • Kim, Kyu-Joong (Department of Biology, Gangneung-Wonju National University) ;
  • Kim, Seung-Bum (Department of Microbiology & Molecular Biology, Chungnam National University) ;
  • Chun, Jong-Sik (School of Biological Sciences, Seoul National University) ;
  • Joh, Ki-Seong (Department of Bioscience and Biotechnology, Hankuk University of Foreign Studies)
  • 김하늘 (한국외국어대학교 자연과학대학 생명공학과) ;
  • 강지영 (전북대학교 자연과학대학 생명과학과) ;
  • 최재희 (인하대학교 자연과학대학 생명과학과) ;
  • 최정욱 (한국외국어대학교 자연과학대학 생명공학과) ;
  • 이상훈 (강릉원주대학교 자연과학대학 생물학과) ;
  • 김태의 (충남대학교 생명시스템과학대학 미생물분자생명과학과) ;
  • 이하나 (서울대학교 자연과학대학 생명과학부) ;
  • 장광엽 (전북대학교 자연과학대학 생명과학과) ;
  • 조장천 (인하대학교 자연과학대학 생명과학과) ;
  • 이현환 (한국외국어대학교 자연과학대학 생명공학과) ;
  • 김규중 (강릉원주대학교 자연과학대학 생물학과) ;
  • 김승범 (충남대학교 생명시스템과학대학 미생물분자생명과학과) ;
  • 천종식 (서울대학교 자연과학대학 생명과학부) ;
  • 조기성 (한국외국어대학교 자연과학대학 생명공학과)
  • Received : 2011.05.26
  • Accepted : 2011.07.19
  • Published : 2011.08.31

Abstract

In this study, samples were collected from the Sumummulbangdui wetland at the Halla Mountain in Jeju Island in order to isolate novel bacterial strain. Bacterial strains belonging to the class Alphaproteobacteria, and Gammaproteobacteria were isolated after spreading samples onto solid agar media. The 16S rRNA gene sequences of the strains assigned to the two classes were compared to those of type strains of the species. The strains that showed less than 98.7% 16S rRNA gene sequence similarity to the validly published species were considered to be novel species candidates. A total of 19 strains were regarded as novel strains which can be regarded as novel species candidates. In the Alphaproteobacteria, 6 novel strains were affiliated with the genera Novosphingobium, and Rhizobium. A total of 13 novel strains belong to Gammaproteobacteria that assigned to the family Moraxellaceae, Pseudomonadaceae, and Enterobacteriaceae were identified. Cultural, physiological, chemotaxonomic characteristics and fatty acids compositions have been determined for the novel species candidates, and the characteristics are described in this study.

제주도 한라산에 위치한 국내 최대규모의 고산습지인 숨은물벵뒤 습지의 Alphaproteobacteria와 Gammaproteobacteria강에 속하는 세균 종의 다양성을 조사하였고, 신분류군 후보 균주 19종을 확보하였다. 신종후보 균주의 16S rRNA 유전자 염기서열을 분석하였을 때 표준균주와 98.7% 미만의 유사도를 보이는 균주들을 신종후보균주로 간주하였다. 총 19종의 세균이 Alphaproteobacteria와 Gammaproteobacteria 강에 속하는 신종후보 균주들로 발견되었다. Alphaproteobacteria 강에 속하는 균주들은 Sphingomonadaceae 과 Novosphingobium 속에 속하는 4종과 Rhizobiaceae 과 Rhizobium 속에 속하는 2종이 분리되었다. Gammaproteobacteria 강에 속하는 균주들은 Moraxellaceae 과 Acinetobacter 속에 속하는 2종, Pseudomonadaceae과 Pseudomonas 속에 속하는 3종, Enterobacteriaceae과의 Enterobacter 속에 속하는 1종, Erwinia 속의 2종, Leclercia 속의 1종, Rahnella 속의 1종, Serratia속의 1종, Yersinia 속의 2종이 분리되었다. 분리된 19개 후보신종에 대하여 배양학적 특징, 생리화학적 특징 및 지방산 분석 결과를 정리하였다.

Keywords

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