Biomolecules & Therapeutics

The Korean Society of Applied Pharmacology (한국응용약물학회)
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Neuroprotective Effect of the n-Hexane Extracts of Laurus nobilis L. in Models of Parkinson's Disease

  • Ham, Ah-Rom (Natural Products Research Institute, College of Pharmacy, Seoul National University) ;
  • Shin, Jong-Heon (Natural Products Research Institute, College of Pharmacy, Seoul National University) ;
  • Oh, Ki-Bong (School of Agricultural Biotechnology, Seoul National University) ;
  • Lee, Sung-Jin (Department of Animal Biotechnology, Kangwon National University) ;
  • Nam, Kung-Woo (Natural Products Research Institute, College of Pharmacy, Seoul National University) ;
  • Koo, Uk (Natural Products Research Institute, College of Pharmacy, Seoul National University) ;
  • Kim, Kyeong-Ho (College of Pharmacy, Kangwon National University) ;
  • Mar, Woong-Chon (Natural Products Research Institute, College of Pharmacy, Seoul National University)
  • Received : 2010.08.16
  • Accepted : 2010.10.18
  • Published : 2011.01.31
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Abstract

Free radical scavenging and antioxidants have attracted attention as a way to prevent the progression of Parkinson's disease (PD). This study was carried out to investigate the effects of n-hexane fraction from Laurus nobilis L. (Lauraceae) leaves (HFL) on dopamine (DA)-induced intracellular reactive oxygen species (ROS) production and apoptosis in human neuroblastoma SH-SY5Y cells. Compared with apomorphine (APO, $IC_{50}=18.1\;{\mu}M$) as a positive control, the HFL $IC_{50}$ value for DA-induced apoptosis was $3.0\;{\mu}g/ml$, and two major compounds from HFL, costunolide and dehydrocostus lactone, were $7.3\;{\mu}M$ and $3.6\;{\mu}M$, respectively. HFL and these major compounds significantly inhibited ROS generation in DA-induced SH-SY5Y cells. A rodent 6-hydroxydopamine (6-OHDA) model of PD was employed to investigate the potential neuroprotective effects of HFL in vivo. 6-OHDA was injected into the substantia nigra of young adult rats and an immunohistochemical analysis was conducted to quantitate the tyrosine hydroxylase (TH)-positive neurons. HFL significantly inhibited 6-OHDA-induced TH-positive cell loss in the substantia nigra and also reduced DA induced $\alpha$-synuclein (SYN) formation in SH-SY5Y cells. These results indicate that HFL may have neuroprotective effects against DA-induced in vitro and in vivo models of PD.

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References

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