Biomolecules & Therapeutics

The Korean Society of Applied Pharmacology (한국응용약물학회)
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Essential Oil of Thujopsis dolobrata Suppresses Atopic Dermatitis-Like Skin Lesions in NC/Nga Mice

  • Nam, Kung-Woo (Natural Products Research Institute, College of Pharmacy, Seoul National University) ;
  • Noh, Jae-Kyu (Natural Products Research Institute, College of Pharmacy, Seoul National University) ;
  • Kim, Su-Kwan (G&G Co., Ltd.) ;
  • Lee, Sung-Jin (Department of Animal Biotechnology, Kangwon National University) ;
  • Kim, Kyeong-Ho (College of Pharmacy, Kangwon National University) ;
  • Oh, Ki-Bong (School of Agricultural Biotechnology, Seoul National University) ;
  • Shin, Jong-Heon (Natural Products Research Institute, College of Pharmacy, Seoul National University) ;
  • Mar, Woong-Chon (Natural Products Research Institute, College of Pharmacy, Seoul National University)
  • Received : 2010.08.14
  • Accepted : 2010.10.11
  • Published : 2011.01.31
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Abstract

We examined the effects of essential oil from Thujopsis dolobrata Sieb. et Zucc. var. hondai Makino (EOTD) (Cupressaceae) on atopic dermatitis (AD)-like skin lesions in NC/Nga mice. Treatment with EOTD twice daily for two weeks ameliorate AD-like skin lesions induced by DNCB (2,4 dinitrochlorobenzene), and clinical scores were reduced to 7.29, 7.07, and 4.5 points in the groups treated with 1.5%, 3.0%, and 6.0% extract (p<0.01) respectively, from the 15.0 score obtained using vehicle. EOTD inhibited the infiltration of mast cells into the AD-like skin lesion in NC/Nga mice (p<0.01) and also reduced serum histamine and IgE levels (p<0.05). Furthermore, it dose-dependently inhibited the release of beta-hexosaminidase from rat basophilic leukemia RBL 2H3 cells. These results indicate that EOTD reduces AD-like skin lesions by inhibiting the production of IgE and histamine, and, thus, IgE-mediated degranulation.

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References

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