생명과학회지 (Journal of Life Science)

  • 제21권1호
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  • Pages.15-21
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  • 2011
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  • 1225-9918(pISSN)
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  • 2287-3406(eISSN)
한국생명과학회 (Korean Society of Life Science)
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한국 내 토마토 재재종의 RAPD에 의한 동정과 유전적 다양성

Identification and Genetic Diversity of Korean Tomato Cultivars by RAPD Markers

  • 투고 : 2010.08.31
  • 심사 : 2010.11.11
  • 발행 : 2011.01.30
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초록

재배종 토마토(Lycopersicum esculentum)는 중요 작물의 하나이다. 36 재배종에 대해 80개 RAPD (random amplified polymorphic DNA) 마커로 동정과 다형성을 조사하였다. 80개 마커 중 36개(45.0%)는 다형성을 나타내었다. 재배종 토마토에서 다형성의 탐지는 유익한 유전자형의 선택에 의한 분자 지도 발전 가능성을 제공할 수 있다. 분자 마커는 역시 식물 육종 지적 권리(PBRs)의 동정과 보호를 위해 사용될 수 있다. 한 예로써 OPC-13 시발체를 사용한 DNA 다형은 Junk Pink와 Ailsa Craighp 품종은 OPC-13-01 밴드가 결여되어 있다. OPA-12-03와 OPB-15-07는 TK-70 품종에 특이 마커로 다른 품종에는 없다. 본 연구의 재배종 토마토에서 DNA 다형은 일부 예외는 있지만 개화 타입과 관련이 있다. 이런 접근은 바람직한 토마토 품종 육성에 유전적 정보를 높이는데 유익할 것으로 사료된다.

Cultivated tomato, Lycopersicum esculentum, is a very important crop. We selected 36 cultivars and studied them for identification and polymorphism by employing random amplified DNA (RAPD) analysis with 80 oligonucleotide primers. Of the 80 primers, 36 primers (45.0%) were polymorphic. Detection of polymorphism in cultivated tomato opens up the possibility of development of its molecular map by judicious selection of genotypes. Molecular markers can also be used for cultivar identification and protection of the plant breeder's intellectual property rights (plant breeders' rights, PBRs). As an example, DNA polymorphism using OPC-13 primer that did not produce the OPC-13-01 band was only found in Junk Pink and Ailsa Craighp cultivars. OPA-12-03 and OPB-15-07 were fragments specific to the TK-70 cultivar and were absent in other cultivars. DNA polymorphism in cultivated tomato in this study was correlated with a type of inflorescence, although some cultivars had exceptions. These approaches will be useful for developing marker-assisted selection tools for genetic enhancement of the tomato plant for desirable traits.

키워드

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피인용 문헌

  1. Assessment of genetic diversity of Mediterranean bread wheat using Randomly Amplified Polymorphic DNA (RAPD) markers vol.9, pp.2, 2011, https://doi.org/10.1016/j.jgeb.2011.10.002