The Korea Journal of Herbology (대한본초학회지)

The Korea Association of Herbology (대한본초학회)
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The Experimental study on the Immuno-regultory effect of Notopterygii Rhizoma Extract on OVA-induced asthma in mice

강활추출물이 OVA-induced Asthma Mouse Model의 면역조절에 미치는 영향

  • Yoo, Ji-Hyun (International Ginseng and Herb Research Institute Geumsan) ;
  • Bae, Jin-Hyun (Department of Herbal Pharmaceutical Science, Joongbu University) ;
  • Doh, Eun-Soo (Department of Herbal Pharmaceutical Science, Joongbu University) ;
  • Chang, Jun-Pok (Department of Herbal Pharmaceutical Science, Joongbu University) ;
  • Seong, Nak-Sul (International Ginseng and Herb Research Institute Geumsan) ;
  • Kil, Ki-Jung (Department of Herbal Pharmaceutical Science, Joongbu University)
  • 유지현 ((재)금산국제인삼약초연구소) ;
  • 배진현 (중부대학교 한방제약과학과) ;
  • 도은수 (중부대학교 한방제약과학과) ;
  • 장준복 (중부대학교 한방제약과학과) ;
  • 성낙술 ((재)금산국제인삼약초연구소) ;
  • 길기정 (중부대학교 한방제약과학과)
  • Received : 2011.11.09
  • Accepted : 2011.12.16
  • Published : 2011.12.30
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Abstract

Objectives : The aim of this study was to investigate the asthma-suppressive and immuno-regulatory effect of Notopterygii Rhizoma(NR) extract on OVA(ovalbumin)-induced asthma in mice. Methods : C57BL/6 mice out of all the experimental groups, except the Normal group and the NRI group, were sensitized and challenged with OVA. C57BL/6 mice were exposed to OVA three times a week for 12 weeks and analyzed by flow cytometer, ELISA, H&E stain. Results : The concentrations of IL-4, IL-5, IL-13, IgE in serum of the OVA-NRII group decreased significantly compared with those of the OVA-Control group. The number of $Gr-1^+/CD11b^+$, $CCR3^+$, $CD3^+/CD19^+$, $CD3e^+/CD69^+$cells in the OVA-NRI group decreased significantly compared with those of the OVA-Control group. The collagen accumulation in the lung sections of the OVA-NRII group decredased signi- ficantly compared with that of the OVA-Control group. Conclusions : These results suggest that Notopterygii Rhizoma(NR) would be a effective candidate for herbal-originated anti-asthmatic drug. However, this drug should be further studied for characterization of the accurate action and underlying mechanism using variant disease model in the future.

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