Parasites, Hosts and Diseases

The Korea Society for Parasitology and Tropical Medicine (대한기생충학·열대의학회)
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Non-specific Defensive Factors of the Pacific Oyster Crassostrea gigas against Infection with Marteilioides chungmuensis: A Flow-Cytometric Study

  • Choi, Hee-Jung (Pathology Division, National Fisheries Research and Development Institute (NFRDI)) ;
  • Hwang, Jee-Youn (Pathology Division, National Fisheries Research and Development Institute (NFRDI)) ;
  • Choi, Dong-Lim (Pathology Division, National Fisheries Research and Development Institute (NFRDI)) ;
  • Huh, Min-Do (Department of Aquatic Life Medicine, Pukyong National University) ;
  • Hur, Young-Baek (Southeast Sea Fisheries Research Institute, NFRDI) ;
  • Lee, Nam-Sil (Pathology Division, National Fisheries Research and Development Institute (NFRDI)) ;
  • Seo, Jung-Soo (Pathology Division, National Fisheries Research and Development Institute (NFRDI)) ;
  • Kwon, Mun-Gyeong (Pathology Division, National Fisheries Research and Development Institute (NFRDI)) ;
  • Choi, Hye-Sung (Pathology Division, National Fisheries Research and Development Institute (NFRDI)) ;
  • Park, Myoung-Ae (Pathology Division, National Fisheries Research and Development Institute (NFRDI))
  • Received : 2011.03.10
  • Accepted : 2011.06.17
  • Published : 2011.09.30
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Abstract

In order to assess changes in the activity of immunecompetency present in Crassostrea gigas infected with Marteilioides chungmuensis (Protozoa), the total hemocyte counts (THC), hemocyte populations, hemocyte viability, and phagocytosis rate were measured in oysters using flow cytometry. THC were increased significantly in oysters infected with M. chungmuensis relative to the healthy appearing oysters (HAO) (P<0.05). Among the total hemocyte composition, granulocyte levels were significantly increased in infected oysters as compared with HAO (P<0.05). In addition, the hyalinocyte was reduced significantly (P<0.05). The hemocyte viability did not differ between infected oysters and HAO. However, the phagocytosis rate was significantly higher in infected oysters relative to HAO (P<0.05). The measurement of alterations in the activity of immunecompetency in oysters, which was conducted via flow cytometry in this study, might be a useful biomarker of the defense system for evaluating the effects of ovarian parasites of C. gigas.

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References

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