청정기술 (Clean Technology)

  • 제17권3호
  • /
  • Pages.273-279
  • /
  • 2011
  • /
  • 1598-9712(pISSN)
  • /
  • 2288-0690(eISSN)
한국청정기술학회 (The Korean Society of Clean Technology)
권호 표지
DOI QR코드

DOI QR Code

서로 다른 물리화학적 특성을 갖는 탄소나노튜브(CNT)의 생물학적 독성 분석

Toxicity Analysis of Carbon Nanotubes Based on Their Physicochemical Properties

  • 김수남 (한국화학연구원 부설 안전성평가연구소) ;
  • 강민성 (한국화학연구원 부설 안전성평가연구소) ;
  • 한영아 (한국화학연구원 부설 안전성평가연구소) ;
  • 김재환 (한국화학연구원 부설 안전성평가연구소) ;
  • 노진규 (광운대학교 화학공학과) ;
  • 김영훈 (광운대학교 화학공학과) ;
  • 최상돈 (아주대학교 분자과학기술학과) ;
  • 박은정 (아주대학교 분자과학기술학과)
  • Kim, Soo-Nam (Inhalation Toxicology Center, KIT Jeongeup Campus) ;
  • Kang, Min-Sung (Inhalation Toxicology Center, KIT Jeongeup Campus) ;
  • Han, Young-Ah (Inhalation Toxicology Center, KIT Jeongeup Campus) ;
  • Kim, Jae-Hwan (Inhalation Toxicology Center, KIT Jeongeup Campus) ;
  • Roh, Jin-Kyu (Department of Chemical Engineering, Kwangwoon University) ;
  • Kim, Young-Hun (Department of Chemical Engineering, Kwangwoon University) ;
  • Choi, Sang-Dun (Department of Molecular Science and Technology, Ajou University) ;
  • Park, Eun-Jung (Department of Molecular Science and Technology, Ajou University)
  • 투고 : 2011.07.14
  • 심사 : 2011.09.18
  • 발행 : 2011.09.30
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

제조나노물질은 원료물질이 동일하더라도 제조방법에 따라 최종생산물의 물리화학적 특성은 매우 상이해질 수 있고 더불어 독성 또한 크게 달라질 수 있을 것으로 추정되고 있다. 본 연구에서 우리는 2종의 탄소나노튜브(단층벽 탄소나노튜브 및 다층벽 탄소나노튜브)를 마우스에 경구와 경기관지로 처리한 후 체중당 상대무게 및 염증반응 지표를 조사함으로써 길이와 전기전도성에 따른 독성을 동정하였다. 실험 결과, 다층벽 탄소나노튜브의 경우 체중 감소는 길이가 짧은 그룹에서 더 강하게 관찰된 반면, 염증 반응은 비교적 길이가 긴 그룹에서 더 강하게 유도되었다. 경기관지로 처리된 단층벽 탄소나노튜브는 뇌와 신장의 상대무게를 의미있게 감소시켰고, 기관지 세척액과 혈액 내 면역세포의 의미있는 변화는 처리 후 1일과 7일에서 주로 관찰되었다. 이 결과를 통해 길이, 입경, 층벽의 수와 같은 탄소나노튜브의 물리화학적 특성이 물질에 의한 독성과 염증 반응에 매우 중요한 인자로 작용하고 있음을 확인할 수 있었다.

The physicochemical properties of manufactured nanomaterials can vary depending upon the methods of manufacture, although the utilized raw materials are same. Hence, the toxicity can also vary based on the methods of nanomaterials manufacture. In this study, we compared the toxicity effect of two types of CNTs (MWCNT, multi-walled carbon nanotube; SWCNT, single-walled carbon nanotube) that differ in length and wall number. In case of MWCNTs, inflammatory responses were more strongly induced in longer groups, whereas body weights more clearly decreased in shorter groups. SWCNT significantly decreased the relative weights of brain and kidney, and the inflow of immune cells and the hematological changes were observed significantly on day 1 and day 7 after exposure, respectively. Our results showed that the length and wall number of CNTs can serve as critical factors in the exhibited inflammation and toxicity.

키워드

참고문헌

  1. Ji, S. R., Liu, C., Zhang, B., Yang, F., Xu, J., Long, J., Jin, C., Fu, D. L., Ni, Q. X., and Yu, X. J., "Carbon Nanotubes in Cancer Diagnosis and Therapy," Biochim. Biophys. Acta, 1806(1), 29-35 (2010).
  2. http://www.oecd.org/document/50/0,3343,en_2649_37015404_ 42059634_1_1_1_37465,00.html
  3. Lam, C. W., James, J. T., McCluskey, R., Arepalli, S., and Hunter, R. L., "A Review of Carbon Nanotube Toxicity and Assessment of Potential Occupational and Environmental Health Risks," Crit. Rev. Toxicol. Mar., 36(3), 189-217 (2006). https://doi.org/10.1080/10408440600570233
  4. Yoshioka, Y., "Nanosafety Studies of Nanomaterials about Biodistribution and Immunotoxicity," Yakugaku Zasshi., 131(2), 221-224 (2011). https://doi.org/10.1248/yakushi.131.221
  5. van derZande, M., Junker, R., Walboomers, X. F., and Jansen, J. A., "Carbon Nanotubes in Animal Models: A Systematic Review on Toxic Potential," Tissue Eng. Part B Rev., 17(1), 57-69 (2011). https://doi.org/10.1089/ten.teb.2010.0472
  6. Kayat, j., Gajbhiye, V., Tekade, R. K., and Jain, N. K., "Pulmonary Toxicity of Carbon Nanotubes: A Systematic Report," Nanomedicine, 7(1), 40-49 (2011). https://doi.org/10.1016/j.nano.2010.06.008
  7. Mutlu, G. M., Budinger, G. R., Green, A. A., Urich, D., Soberanes, S., Chiarella, S. E., Alheid, G. F., McCrimmon, D. R., Szleifer, I., and Hersam, M. C., "Biocompatible Nanoscale Dispersion of Single-walled Carbon Nanotubes Minimizes in Vivo Pulmonary Toxicity," Nano Lett., 10(5), 1664-1670 (2010). https://doi.org/10.1021/nl9042483
  8. Johnston, H. J., Hutchison, G. R., Christensen, F. M., Peters, S., Hankin, S., Aschberger, K., and Stone, V., "A Critical Review of the Biological Mechanisms Underlying the in Vivo and in Vitro Toxicity of Carbon Nanotubes: The Contribution of Physico-chemical Characteristics," Nanotoxicology, 4(2), 207-246(2010). https://doi.org/10.3109/17435390903569639
  9. Kim, M., Choi, K., Kim, Y., and Yi, J., "Risk Assessment for Health and Environmental Hazards of Nanomaterials," Clean Technology, 13(3), 161-172 (2007).
  10. Tabet, L., Bussy, C., Setyan, A., Simon-Deckers, A., Rossi, M. J., Boczkowski, J., and Lanone, S., "Coating Carbon Nanotubes with a Polystyrene-based Polymer Protects Against Pulmonary Toxicity," Part Fibre Toxicol., 8, 3 (2011). https://doi.org/10.1186/1743-8977-8-3
  11. Yamashita, K., Yoshioka, Y., Higashisaka, K., Morishita, Y., Yoshida, T., Fujimura, M., Kayamuro, H., Nabeshi, H., Yamashita, T., Nagano, K., Abe, Y., Kamada, H., Kawai, Y., Mayumi, T., Yoshikawa, T., Itoh, N., Tsunoda, S., and Tsutsumi, Y., "Carbon Nanotubes Elicit DNA Damage and Inflammatory Response Relative to Their Size and Shape," Inflammation, 33(4), 276-280 (2010). https://doi.org/10.1007/s10753-010-9182-7
  12. Donaldson, K., Murphy, F., Schinwald, A., Duffin, R., and Poland, C.A., "Identifying the Pulmonary Hazard of High Aspect Ratio Nanoparticles to Enable Their Safety-by-design," Nanomedicine (Lond)., 6(1), 143-156 (2011). https://doi.org/10.2217/nnm.10.139
  13. Donaldson, K., Murphy, F. A., Duffin, R., and Poland, C. A. "Asbestos, Carbon Nanotubes and the Pleural Mesothelium: A Review of the Hypothesis Regarding the Role of Long Fibre Retention in the Parietal Pleura, Inflammation and Mesothelioma." Part Fibre Toxicol., 7, 5 (2010). https://doi.org/10.1186/1743-8977-7-5
  14. Pacurari, M., Castranova, V., and Vallyathan, V. "Single- and Multi-wall Carbon Nanotubes Versus Asbestos: Are the Carbon Nanotubes a New Health Risk to Humans?," J. Toxicol. Environ. Health A, 73(5), 378-395 (2010). https://doi.org/10.1080/15287390903486527
  15. Park, E. J., Cho W. S., Jeong J, Yi, J., Choi, K., and Park, K., "Pro-inflammatory and Potential Allergic Responses Resulting from B Cell Activation in Mice Treated with Multi-walled Carbon Nanotubes by Intratracheal Instillation," Toxicology, 259(3), 113-121 (2009). https://doi.org/10.1016/j.tox.2009.02.009
  16. Park, E. J., Roh, J. Kim, S. N., Kim, Y., Hong, J. T., and Choi, K., "A Single Intratracheal Instillation of Single-walled Carbon Nanotubes Induced Early Lung Fibrosis and Subchronic Tissue Damage in Mice," Arch. Toxicol., 85(9), 1121-1131 (2011). https://doi.org/10.1007/s00204-011-0655-8