Progress in Medical Physics (한국의학물리학회지:의학물리)

Korean Society of Medical Physics (한국의학물리학회)
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Small Animal PET Imaging Study of 68Ga-BAPEN

68Ga-BAPEN 소동물 PET영상 연구

  • Kim, Ji-Who (Department of Nuclear Medicine, Interdisciplinary Program in Radiation Applied Life Science, Seoul National University) ;
  • Lee, Jae-Sung (Department of Nuclear Medicine, Interdisciplinary Program in Radiation Applied Life Science, Seoul National University) ;
  • Yang, Bo-Yeun (Department of Nuclear Medicine, Interdisciplinary Program in Radiation Applied Life Science, Seoul National University) ;
  • Kim, Su-Jin (Department of Nuclear Medicine, Interdisciplinary Program in Radiation Applied Life Science, Seoul National University) ;
  • Kim, Joong-Hyun (Department of Nuclear Medicine, Interdisciplinary Program in Radiation Applied Life Science, Seoul National University) ;
  • Jeong, Jae-Min (Department of Nuclear Medicine, Interdisciplinary Program in Radiation Applied Life Science, Seoul National University) ;
  • Lee, Dong-Soo (Department of Nuclear Medicine, Interdisciplinary Program in Radiation Applied Life Science, Seoul National University)
  • 김지후 (서울대학교 의과대학 핵의학교실, 협동과정 방사선응용생명과학) ;
  • 이재성 (서울대학교 의과대학 핵의학교실, 협동과정 방사선응용생명과학) ;
  • 양보연 (서울대학교 의과대학 핵의학교실, 협동과정 방사선응용생명과학) ;
  • 김수진 (서울대학교 의과대학 핵의학교실, 협동과정 방사선응용생명과학) ;
  • 김중현 (서울대학교 의과대학 핵의학교실, 협동과정 방사선응용생명과학) ;
  • 정재민 (서울대학교 의과대학 핵의학교실, 협동과정 방사선응용생명과학) ;
  • 이동수 (서울대학교 의과대학 핵의학교실, 협동과정 방사선응용생명과학)
  • Received : 2011.09.28
  • Accepted : 2011.12.01
  • Published : 2011.12.30
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Abstract

The purpose of this study was to analyze $^{68}Ga$-BAPEN dynamic PET image in rat myocardium to evaluate potential of this radiotracer as a perfusion imaging agent. Animal PET/CT scan was done in 9 rats during 120 minutes. Especially we synthesized $^{68}Ga$-BAPEN with kit which is simple and low cost method. PET images showed the in vivo dynamic distribution of $^{68}Ga$-BAPEN in the chest region of rats. Initially $^{68}Ga$-BAPEN PET images showed aorta and liver activities and a few minutes later, $^{68}Ga$-BAPEN moved to myocardium. Regions of interest were drawn on myocardium, liver, lung and blood pool. Time-activity curves showed significant uptake of $^{68}Ga$-BAPEN in myocardium. The contrast ratios of myocardial to blood pool, lung and liver at 60 minutes after injection were 1.66, 2.82 and 0.60. To estimate accurate kinetic parameters, 60 minutes after injection was required to PET scan as myocardium image contrast ratios reached to constant values. As a result, $^{68}Ga$-BAPEN would be suitable radiotracer for PET which can applied to diagnosis of myocardial perfusion diseases after further preclinical and clinical investigations.

본 연구에서는 소동물의 심근에서 $^{68}Ga$-BAPEN PET 영상분석을 통해 심혈 영상 추적자로서의 적용가능성을 보고자 하였다. 소동물용 PET/CT에서 쥐 9마리를 대상으로 120분간의 $^{68}Ga$-BAPEN PET/CT 스캔을 시행하였다. 특별히 킷트를 통해 간편하고 저비용으로 $^{68}Ga$-BAPEN을 합성이 가능하였다. PET 영상은 쥐의 몸통부분에서 $^{68}Ga$-BAPEN의 생체 동적분포를 나타낸다. $^{68}Ga$-BAPEN PET 영상은 처음 수분간 대동맥과 간에서의 섭취가 나타났고 점차 심근에서의 섭취가 이루어졌다. 관심영역은 좌심근, 심혈, 폐, 간에 그렸고 시간-방사능 곡선을 얻었다. 시간-방사능 곡선에서 $^{68}Ga$-BAPEN이 쥐 심근에 잘 결합하는 것을 확인 할 수 있었다. 정확한 약동학적 파라미터 도출을 위한 최소 PET 스캔시간은 타장 기와의 영상 대조도가 일정비에 이르는 주사 후 60분이 적합하였다. 이때 심근의 섭취를 심혈, 간, 폐에서의 섭취로 나누어 얻은 영상 대조도는 각각 1.66, 0.60, 2.82였다. 결론적으로 $^{68}Ga$-BAPEN은 심근 혈류 질환을 진단하기 위한 추적자로서 적합하며 지속적인 연구가 이루어진다면 임상에서의 진단활용에 도움이 될 것이라 예상된다.

Keywords

References

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