Bulletin of the Korean Chemical Society

  • 제32권1호
  • /
  • Pages.149-152
  • /
  • 2011
  • /
  • 0253-2964(pISSN)
  • /
  • 1229-5949(eISSN)
대한화학회 (Korean Chemical Society)
권호 표지
DOI QR코드

DOI QR Code

Development of Novel Method for the Detection of Microcystin Using Chemiluminescence Immunochromatography

  • Pyo, Dong-Jin (Department of Chemistry, Kangwon National University) ;
  • Yoo, Ji-Sun (Department of Chemistry, Kangwon National University)
  • 투고 : 2010.07.07
  • 심사 : 2010.11.04
  • 발행 : 2011.01.20
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

A new chemiluminescence immunochromatographic analysis system with high sensitivity and high reproducibility was developed for the determination of microcystins (MCs) in water. Horse radish peroxidase (HRP) labeled microcystin monoclonal antibody was used for the sensitive chemiluminescence detection. The chemiluminescence immunochromatographic analysis system was composed of microcystin LR (MCLR)-monoclonal antibody (mAb)-Horse Radish Peroxidase (HRP) conjugate, MCLR-BSA conjugate, luminol, hydrogen peroxide mixture solution, an immunochromatographic assay strip and luminometer. To detect the concentration of microcystins in water, we utilized one spot analysis of the strip instead of flow type analysis. We could detect the microcystins in water at a concentration as low as 9.45 pg/mL with the chemiluminescence (CL) detection.

키워드

참고문헌

  1. Thorpe, G. H. G.; Kricka, L. J. In Biolumin. Chemilumin.: New Perspectives; Scholmerich, J. R., Andreesen, R., Kapp, A., Ernst, M., Woods, W., eds.; Chichester: J. Wiley. P. 1987; pp 199-208.
  2. Paradies, H. H.; Gesellschaft, B. D. B. Phys. Chem. 1992, 96, 1027.
  3. Zhang, X. R.; Baeyens, W. R. G.; Garcia-Campana, A. M.; Quyang, J. Trends Anal. Chem. 1999, 18, 6.
  4. Maccapra, F. Oxford University Press: Oxford, 1984; pp 617-637.
  5. Pyo, D.; Huang, Y.; Kim, Y.; Hahn, J. H. Bull. Korean Chem. Soc. 2005, 26, 939. https://doi.org/10.5012/bkcs.2005.26.6.939
  6. Oh, H. M.; Lee, S. J.; Kim, J. H.; Yoon, B. D. Appl. Envir. Micro. 2002, 67, 1484.
  7. Pyo, D.; Yoo, J. J. Immun. Immunochem. 2008, 29, 211. https://doi.org/10.1080/15321810802122202
  8. Ha, S. G.; Park, J. B.; Ko, K. K.; Choi, E. Y. Hybridoma. 1999, 18, 371. https://doi.org/10.1089/hyb.1999.18.371
  9. Pyo, D.; Moon D. Bull. Korean Chem. Soc. 2005, 26, 2089. https://doi.org/10.5012/bkcs.2005.26.12.2089
  10. Pyo, D.; Yoo, J. Anal. Lett. 2007, 40, 3208. https://doi.org/10.1080/00032710701672673
  11. Egorov, A. M.; Kim, B. B.; Pisarev, V. V.; Kapeliuch, Yu. L.; Gazarian, I. G. In Biolum. and Chemilum. Status report; Szalay, A. A., Stanly, P. E., Kricka, L. J., eds.; Chichester: Wiley. P. 1993; pp 286-290.
  12. Yamaguchi, M.; Yoshida, H.; Nohta, H. J. Chromatogr. A 2002, 950, 1. https://doi.org/10.1016/S0021-9673(02)00004-3
  13. White, E. H., McElroy, W. D., Glass, B., Eds.; Light and Life, John Hopkins Press: Baltimore, 1961; pp 183-195.
  14. White, E. H.; Bursey, M. M. J. Am. Chem. Soc. 1964, 86, 941. https://doi.org/10.1021/ja01059a051
  15. White, E. H.; Zafiriou, O.; Kagi, H. H.; Hill, J. H. M. J. Am. Chem. Soc. 1964, 86, 940. https://doi.org/10.1021/ja01059a050
  16. Gundermann, K. D.; McCapra, F. Chemiluminescence in Organic Chemistry; Springer-Verlag: Berlin, 1987.
  17. Barni, F.; Lewis, S. W.; Berti, A.; Miskelly, G. M.; Lago, G.; Talanta 2007, 72, 896. https://doi.org/10.1016/j.talanta.2006.12.045
  18. Congying, G.; Li, L.; Nenghu, F.; Jinping, J. Chin. J. Chromatogr. 2007, 25, 174. https://doi.org/10.1016/S1872-2059(07)60005-4
  19. Bouarcha, N.; Maatouk, I.; Vincent, G.; Levi, Y. Toxicol. 2002, 40, 1677.