한국임상수의학회지 (Journal of Veterinary Clinics)

  • 제28권5호
  • /
  • Pages.473-478
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  • 2011
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  • 1598-298X(pISSN)
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  • 2384-0749(eISSN)
한국임상수의학회 (The Korean Society of Veterinary Clinics)
권호 표지

개 마취와 수술 창상에 따른 산화스트레스에 대한 비타민 C의 효과

Effects of Vitamin C on Oxidative Stress Due to Anesthesia and Surgical Trauma in Dogs

  • 최경하 (충남대학교 수의과대학) ;
  • 이재연 (충남대학교 수의과대학) ;
  • 정성목 (충남대학교 수의과대학) ;
  • ;
  • 김명철 (충남대학교 수의과대학)
  • Choi, Kyeong-Ha (College of Veterinary Medicine, Chungnam National University) ;
  • Lee, Jae-Yeon (College of Veterinary Medicine, Chungnam National University) ;
  • Jeong, Seong-Mok (College of Veterinary Medicine, Chungnam National University) ;
  • Joo, Gregory K. (Cottman Animal Hospital) ;
  • Kim, Myung-Cheol (College of Veterinary Medicine, Chungnam National University)
  • 심사 : 2011.10.06
  • 발행 : 2011.10.31
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초록

이 연구는 개에서 isoflurane을 이용한 전신마취 하에 개복술시 아스코르빈산 (AA)의 항산화 효과를 평가하였다. 12마리의 개를 아스코르빈산 그룹 (AAG) 또는 sham 그룹 (SG)으로 무작위 배정하였다. AAG는 마취 10분 전 에 표준 아스코르빈산의 100 mg/animal 복용량을 정맥주사하였다. 혈장에 있는 코티졸, 포도당, total oxidant status (TOS), total antioxidant status (TAS)와 oxidative stress index (OSI)를 측정하였다. 코티졸 수치는 두 그룹에서 시간이 지남에 따라 유의성있게 증가하였다 (p < 0.05). 포도당 수치의 변화는 두 그룹 사이에 유의성이 없었다. TOS와 OSI는 SG에서 시간이 지남에 따라 유의성 있게 증가하였다 (p < 0.05). 그런데 AAG에서는 유의성있는 변화가 없었다. 그리고, AAG의 TOS와 OSI는 SG에서 보다 더 유의성 있게 낮았다 (p < 0.05). 그러나 TAS는 두 그룹 사이에 유 의성이 있는 변화가 없었다. 이런 결과는 AAG에서 TOS 감소가 항산화제에서 산화제로의 변환과 관련 있음을 예측 해 볼 수 있게 한다. OSI 감소는 손상부위에 발생한 활성산소 (ROS, 즉 산화 스트레스)의 감소가 혈액순환 이상, 기관 부전 및 염증의 수술 부작용을 줄일 수 있다는 것을 의미한다. 그러므로, AA는 개의 개복술에서 산화 스트레스로부터 수술환자를 보호하기 위하여 사용될 수 있다.

The study evaluated the antioxidant effects of ascorbic acid (AA) against oxidative stress during laparotomy in dogs under general anesthesia using isoflurane. Twelve dogs were randomly assigned to either the ascorbic acid group (AAG) or the sham group (SG). AAG received intravenous dosage of 100 mg/animal of standard AA 10 minutes before anesthesia. Plasma levels of cortisol, glucose, total oxidant status (TOS), total antioxidant status (TAS), and oxidative stress index (OSI) were measured. Cortisol level increased significantly with time in both groups (p < 0.05). The change of glucose levels was not significantly different between both groups. TOS and OSI increased significantly with time in the SG (p < 0.05), whereas it did not significantly change with time in the AAG. The TOS and OSI of the AAG were significantly lower than in the SG (p < 0.05). However, TAS did not significantly change between both groups. The results suggest that the decrease of TOS in the AAG might be related to the conversion of antioxidants to oxidants. The decrease of OSI indicates that the decrease of reactive oxygen species (i.e., oxidative stress) produced at the site of injury is able to decrease surgical side effects of abnormal blood circulation, organ failure, and inflammation. Therefore, AA can be used to protect a surgical patient from oxidative stress in canine laparotomy.

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참고문헌

  1. Bartels M, Vilsendorf AM, Kassahun1 WT, Gerstenbergk B, Engelhart K, Vilsendorf EM, Faber S, Biesalski HK. Protective effect of antioxidative vitamins against lipid peroxidation in liver ischemia and reperfusion - an animal experimental study. EXCLI J 2007; 6: 152-166.
  2. Baysal Z, Togrul T. Evaluation of total oxidative and antioxidative status in pediatric patients undergoing laparoscopic surgery. J Pediatr Surg. 2009; 44: 1367-1370. https://doi.org/10.1016/j.jpedsurg.2008.11.031
  3. Behrendt D, Beltrame J, Hikiti H, Wainstein M, Kinlay S, Selwyn AP, Ganz P, Fang JC. Impact of coronary endothelial function on the progression of cardiac transplant-associated arteriosclerosis: effect of anti-oxidant vitamins C and E. J Heart Lung Transplant 2006; 25: 426-433. https://doi.org/10.1016/j.healun.2005.11.450
  4. Berger MM, Shenkin A. Update on clinical micronutrient supplementation studies in the critically ill. Curr Opin Clin Nutr Metab Care 2006; 9: 711-716. https://doi.org/10.1097/01.mco.0000247466.41661.ba
  5. Bulger EM, Maier RV. Antioxidants in critical illness. Arch Surg 2001; 136: 1201-1207. https://doi.org/10.1001/archsurg.136.10.1201
  6. Buyukhatipoglu H, Sezen Y, Yildiz A, Bas M, Kirhan I, Ulas T, Turan MN, Taskin A, Aksoy N. N-acetylcysteine fails to prevent renal dysfunction and oxidative stress after noniodine contrast media administration during percutaneous coronary interventions. Pol Arch Med Wewn 2010; 120: 383-389.
  7. Caryl Goodyear-Bruch and Janet D Pierce. Oxidative Stress in Critically Ill Patients. Am J Crit Care 2002; 11: 543-551.
  8. Cuzzocrea S, Riley DP, Caputi AP, Salvemini D. Antioxidant therapy: a new pharmacological approach in shock, inflammation, and ischemia / reperfusion injury. Pharmacol Rev 2001; 53: 135-159.
  9. De Vries AP, Oterdoom LH, Gans RO, Bakker SJ. Supplementation with anti-oxidants Vitamin C and E decreases cyclosporine A trough-levels in renal transplant recipients. Nephrol Dial Transplant 2006; 21: 231-232. https://doi.org/10.1093/ndt/gfi112
  10. Dodam JR, Kruse-Elliott KT, Aucoin DP, Swanson CR. Duration of etomidate-induced adrenocortical suppression during surgery in dogs. Am J Vet Res 1990; 51: 786-788.
  11. Egwu GO, Mshelia GD, Sanni S, Onyeyili PA, Adeyanju GT. The effect of vitamin C at varying times on physiological parameters in rabbits after xylazine anaesthesia. Vet Ital 2011; 47: 97-104.
  12. Elsa A, Ubandawaki S. Ketamine anaesthesia following premedication of rabbits with vitamin C. J Vet Sci 2005; 6: 239-241.
  13. Erel O. A novel automated direct measurement method for total antioxidant capacity using a new generation, more stable ABTS radical cation. Clin Biochem 2004; 37: 277-285. https://doi.org/10.1016/j.clinbiochem.2003.11.015
  14. Fukushima R, Yamazaki E. Vitamin C requirement in surgical patients. Curr Opin Clin Nutr Metab Care 2010; 13: 669-676. https://doi.org/10.1097/MCO.0b013e32833e05bc
  15. Gunel E, Caglayan F, Caglayan O, Dilsiz A, Duman S, Aktan M. Treatment of intestinal reperfusion injury using antioxidative agents. J Pediatr Surg 1998; 33: 1536-1539. https://doi.org/10.1016/S0022-3468(98)90492-4
  16. Gutteridge J, Mitchell J. Redox imbalance in the critically ill. Br Med Bull 1999; 55: 49-75. https://doi.org/10.1258/0007142991902295
  17. Kohen R, Nyska A. Oxidation of biological systems: oxidative stress phenomena, antioxidants, redox reactions, and methods for their quantification. Toxicol Pathol 2002; 30: 620-650. https://doi.org/10.1080/01926230290166724
  18. Koksal H, Kurban S. Total oxidant status, total antioxidant status, and paraoxonase and arylesterase activities during laparoscopic cholecystectomy. Clinics (Sao Paulo) 2010; 65: 285-290. https://doi.org/10.1590/S1807-59322010000300008
  19. Lagowska-Lenard M, Stelmasiak Z, Bartosik-Psujek H. Influence of vitamin C on markers of oxidative stress in the earliest period of ischemic stroke. Pharmacol Rep 2010; 62: 751-756. https://doi.org/10.1016/S1734-1140(10)70334-0
  20. Lee JI, Son HY, Kim MC. Attenuation of ischemiareperfusion injury by ascorbic acid in the canine renal transplantation. J Vet Sci 2006; 7: 375-379. https://doi.org/10.4142/jvs.2006.7.4.375
  21. Marana E, Scambia G, Maussier ML, Parpaglioni R, Ferrandina G, Meo F, Sciarra M, Marana R. Neuroendocrine stress response in patients undergoing benign ovarian cyst surgery by laparoscopy, minilaparotomy, and laparotomy. J Am Assoc Gynecol Laparosc 2003; 10: 159-165. https://doi.org/10.1016/S1074-3804(05)60291-5
  22. Nathens AB, Neff MJ, Jurkovich GJ, Klotz P, Farver K, Ruzinski JT, Radella F, Garcia I, Maier RV. Randomized, prospective trial of antioxidant supplementation in critically ill surgical patients. Ann Surg 2002; 236: 814-822. https://doi.org/10.1097/00000658-200212000-00014
  23. Papich MG. Handbook of veterinary drugs. 1st ed, Philadelphia: Saunders. 2002: 31.
  24. Pirbudak L, Balat O, Cekmen M, Ugur MG, Aygun S, Oner U. Effect of ascorbic acid on surgical stress response in gynecologic surgery. Int J Clin Pract 2004; 58: 928-931. https://doi.org/10.1111/j.1742-1241.2004.00022.x
  25. Ryu HY, Lee CJ, Soh DM. Ho Choi, Ihm SH. Effects of vitamin C as antioxidant on recovery of left ventricular function after ischemia and reperfusion in isolated rat heart. Korean J Thorac Cardiovasc Surg 1996; 593-598.
  26. Sagsoz N, Kisa U, Apan A. Ischaemia-reperfusion injury of rat ovary and the effects of vitamin C, mannitol and verapamil. Hum Reprod 2002; 17: 2972-2976. https://doi.org/10.1093/humrep/17.11.2972
  27. Sasaki M, Joh T. Oxidative stress and ischemia-reperfusion injury in gastrointestinal tract and antioxidant, protective agents. J Clin Biochem Nutr 2007; 40: 1-12.
  28. Trachootham D, Lu W, Ogasawara MA, Nilsa RD, Huang P. Redox regulation of cell survival. Antioxid Redox Signal 2008; 10: 1343-1374. https://doi.org/10.1089/ars.2007.1957
  29. Tsuchiya M, Sato EF, Inoue M, Asada A. Open abdominal surgery increases intraoperative oxidative stress: can it be prevented. Anesth Analg 2008; 107: 1946-1952. https://doi.org/10.1213/ane.0b013e318187c96b
  30. Ullegaddi R, Powers HJ, Gariballa SE. Antioxidant supplementation enhances antioxidant capacity and mitigates oxidative damage following acute ischaemic stroke. Eur J Clin Nutr. 2005; 59: 1367-1373. https://doi.org/10.1038/sj.ejcn.1602248
  31. Van Stijn MF, Ligthart-Melis GC, Boelens PG, Scheffer PG, Teerlink T, Twisk JW, Houdijk AP, Van Leeuwen PA. Antioxidant enriched enteral nutrition and oxidative stress after major gastrointestinal tract surgery. World J Gastroenterol 2008; 14: 6960-6969. https://doi.org/10.3748/wjg.14.6960