Journal of the East Asian Society of Dietary Life (동아시아식생활학회지)

The East Asian Society of Dietary Life (동아시아식생활학회)
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Optimization of Extraction Conditions for Functional Components from Acai (Euterpe oleracea Mart.) by Response Surface Methodology

반응표면분석에 의한 Acai(Euterpe oleracea Mart.) 기능성분의 추출조건 최적화

  • Hong, Joo-Heon (Dept. of Food Science and Technology, Catholic University of Daegu)
  • 홍주헌 (대구가톨릭대학교 식품가공학)
  • Received : 2011.09.01
  • Accepted : 2011.10.17
  • Published : 2011.10.31
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Abstract

This study was conducted to monitor the quality characteristics of Acai (Euterpe oleracea Mart.) ethanolic extracts by a response surface methodology. The independent variables in the extraction experiments were ethanol concentration (0~100%), extraction temperature ($35{\sim}95^{\circ}C$), and ratio of solvent to sample (10~30 mL/g). The coefficients of determinations ($R^2$) were 0.9596 (p<0.01), 0.9356 (p<0.01), and 0.8842 (p<0.05) for total polyphenol, total flavonoid, and electron donating ability, respectively. The electron donating ability and nitrite scavenging effect improved with an increase in ethanol concentration as opposed to extraction temperature. Anthocyanin content with extraction conditions was 74.421~291.841 mg/L and the coefficient of determinations ($R^2$) was 0.9792 (p<0.01). ORAC (oxygen radical absorbance capacity) with extraction conditions was 137.73~562.94 ${\mu}moles$ TE/g and increased with an increase in ethanol concentration and a decrease in the ratio of solvent to sample content. Estimated conditions for maximum extraction including yield, total polyphenol, total flavonoid, electron donating ability, anthocyanin content, and ORAC were 28~58% for ethanol concentration, $60{\sim}68^{\circ}C$ for extraction temperature, and 10~12 mL/g for ratio of solvent to sample.

본 실험은 반응표면분석법을 이용하여 추출조건에 따른 Acai 에탄올 추출물의 이화학적 특성을 모니터링하였다. 추출조건에 따른 수율의 최대값은 2.84%이었고, 중심합성계획에 의한 Acai 추출조건별 총 폴리페놀 및 총 플라보노이드 함량에 대한 반응표면 회귀식의 $R^2$는 각각 0.9596와 0.9356로 1% 이내의 유의수준에서 유의성이 인정되었다. 전자공여능에 대한 회귀식의 $R^2$는 0.8842로 5% 이내의 유의수준에서 유의성이 인정되었으며, 추출온도보다는 에탄올 농도에 크게 영향을 받고 있는 것으로 나타났다. 아질산염 소거능에 대한 pH 조건별 추출조건의 영향은 에탄올 농도가 가장 영향이 큰 것으로 나타났다. 추출조건별 anthocyanin 함량은 74.421~291.841 mg/L의 범위였으며, 결과에 대한 반응표면 회귀식의 $R^2$는 0.9792로 1% 이내의 유의수준에서 인정되었고, 추출온도와 시료에 대한 용매비보다는 에탄올 농도에 많은 영향을 받음을 알 수 있었다. ORAC은 137.73~562.94 ${\mu}moles$ TE/g의 범위였으며, 시료에 대한 용매비가 낮을수록, 에탄올농도가 높을수록 함량이 증가하였다. Acai의 추출조건을 최적화하기 위하여 추출물의 특성인 추출물의 수율, 총폴리페놀 함량, 총 플라보노이드 함량, 전자공여능, anthocyanin 함량 및 ORAC에 대해 contour map을 superimposing하여 얻은 최적추출조건의 범위는 에탄올 농도 28~58%, 추출온도 $60{\sim}68^{\circ}C$, 시료에 대한 용매비가 10~12 mL/g이었다.

Keywords

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