Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
권호 표지
DOI QR코드

DOI QR Code

Molecular Genetic Identification of Yeast Strains Isolated from Egyptian Soils for Solubilization of Inorganic Phosphates and Growth Promotion of Corn Plants

  • Received : 2010.06.26
  • Accepted : 2010.09.20
  • Published : 2011.01.28
Download PDF
⟨ Previous Next ⟩

Abstract

Forty yeast strains isolated from soils taken from different locations in Egypt were tested for their P-solubilizing activities on the basis of analyzing the clear zone around colonies growing on a tricalcium phosphate medium after incubation for 5 days at $25^{\circ}C$, denoted as the solubilization index (SI). Nine isolates that exhibited P-solubilization potential with an SI ranging from 1.19 to 2.76 were genetically characterized as five yeasts belonging to the genus Saccharomyces cerevisiae and four non-Saccharomyces, based on a PCR analysis of the ITS1-26S region amplied by SC1/SC2 species-specific primers. The highest P-solubilization efficiency was demonstrated by isolate PSY- 4, which was identified as Saccharomyces cerevisiae by a sequence analysis of the variable D1/D2 domain of the 26S rDNA. The effects of single and mixed inoculations with yeast PSY-4 and Bacillus polymyxa on the P-uptake and growth of corn were tested in a greenhouse experiment using different levels of a phosphorus chemical fertilizer (50, 100, and 200 kg/ha super phosphate 15.5% $P_2O_5$). The results showed that inoculating the corn with yeast PSY-4 or B. polymyxa caused significant increases in the shoot and root dry weights and P-uptake in the shoots and roots. The P-fertilization level also had a significant influence on the shoot and root dry weights and P-uptake in the shoots and roots when increasing the P-level from 50 up to 200 kg/ha. Dual inoculation with yeast strain PSY-4 and B. polymyxa at a P-fertilization level of 200 kg/ha gave higher values for the shoot and root dry weights and P-uptake in the shoots and roots, yet these increases were nonsignificant when compared with dual inoculation with yeast strain PSY-4 and B. polymyxa at a P-fertilization level of 100 kg/ha. The best increases were obtained from dual inoculation with yeast strain PSY-4 and B. polymyxa at a P-fertilization level of 100 kg/ha, which induced the following percentage increases in the shoot and root dry weights, and P-uptake in the shoots and roots; 16.22%, 46.92%, 10.09%, and 31.07%, respectively, when compared with the uninoculated control (fertilized with 100 kg/ha).

Keywords

References

  1. Ahuja, A., S. B. Ghosh, and S. F. D'Souza. 2007. Isolation of a starch utilizing, phosphate solubilizing fungus on buffered medium and its characterization. Bioresource Technol. 98: 3408-3411. https://doi.org/10.1016/j.biortech.2006.10.041
  2. Arpana, N., S. D. Kumar, and T. N. Prasad. 2002. Effect of seed inoculation, fertility and irrigation on uptake of major nutrients and soil fertility status after harvest of late sown lentil. J. Appl. Biol. 12: 23-26.
  3. Cappello, M. P., G. Bleve, F. Grieco, F. Dellaglio, and F. Zacheo. 2004. Characterization of Saccharomyces cerevisiae strains isolated from must of grape grown in experimental vineyard. J. Appl. Microbiol. 97: 1274-1280. https://doi.org/10.1111/j.1365-2672.2004.02412.x
  4. De Freitas, R. J. 2000. Yield and N assimilation of winter wheat (Triticum aestivum L., var Norstar) inoculated with rhizobacteria. Pedobiologia 44: 97-104. https://doi.org/10.1078/S0031-4056(04)70031-1
  5. Edi Premono, M., A. M. Moawad, and P. L. Velk. 1996. Effect of phosphate-solubilizing Pseudomonas putida on the growth of maize and its survival in the rhizosphere. Indones. J. Crop Sci. 11: 13-23.
  6. Finogenova, T. V., I. G. Mordunov, S V. Kamzolova, and O. G. Chernyavskaya. 2005. Organic acid production by the yeast Yarrowia lipolytica; A review of prospects. Appl. Biochem. Microbiol. 41: 418-425. https://doi.org/10.1007/s10438-005-0076-7
  7. Frutos, R. L., M. T. Fernandez-Espinar, and A. Querol. 2004. Identification of species of the genus Candida by analysis of the 5.8S rRNA gene and the two ribosomal internal transcribed spacers. Antonie Van Leeuwenhoek 85: 175-185. https://doi.org/10.1023/B:ANTO.0000020154.56649.0f
  8. Goddard, V. J., M. J. Bailey, P. Darrah, A. K. Lilley, and L. P. Thompson. 2001. Monitoring temporal and spatial variation in rhizosphere bacterial population diversity: A community approach for the improved selection of rhizosphere competent bacteria. Plant Soil 231: 181-193.
  9. Goldstein, A. H. 1986. Bacterial solubilization of mineral phosphates: Historical perspectives and future prospects. Am. J. Altern. Agric. 1: 51-57. https://doi.org/10.1017/S0889189300000886
  10. Guimaraes, T. M., D. G. Moriel, I. P. Machado, C. M. T. F. Picheth, and T. M. B. Bonfim. 2006. Isolation and characterization of Saccharomyces cerevisiae strains of winery interest. Braz. J. Pharm. Sci. 42: 119-126. https://doi.org/10.1002/jps.3030420219
  11. Halder, A. K., A. K. Mishra, and P. K. Chakarbarthy. 1991. Solubilization of inorganic phosphate by Bradyrhizobium. Ind. J. Exp. Biol. 29: 28-31.
  12. Hamdali, H., M. Hafidi, M. J. Virolle, and Y. Ouhdouch. 2008. Rock phosphate solubilizing actinomycetes: Screening for plant growth-promoting activities. World J. Microbiol. Biotechnol. 24: 2565-2575. https://doi.org/10.1007/s11274-008-9817-0
  13. Hamdali, H., A. Smirnov, C. Esnault, Y. Ouhdouch, and J. Virolle. 2010. Phosiological studies and comparative analysis of rock phosphate solubilization abilities of Actinomycetales originating from Moroccan phosphate mines and of Streptomyces lividans. Appl. Ecol. 44: 24-31.
  14. Harju, S., H. Fedosyuk, and K. R. Peterson. 2004. Rapid isolation of yeast genomic DNA: Bust n' Grab. BMC Biotechnol. 4: 8. https://doi.org/10.1186/1472-6750-4-8
  15. Hong, S. G., J. Chun, H. W. Oh, and K. S. Bae. 2001. Metschnikowia koreensis sp. nov., a novel yeast species isolated from flowers in Korea. Int. J. Syst. Evol. Microbiol. 51: 1927-1931. https://doi.org/10.1099/00207713-51-5-1927
  16. Huang, C. H., F. L. Lee, and C. J. Tai. 2009. The b-tubulin gene as a molecular phylogenetic marker for classification and discrimination of the Saccharomyces sensu stricto complex. Antonie Van Leeuwenhoek 95: 135-142.
  17. Jackson, M. L. 1973. Soil Chemical Analysis. Prentice-Hall, India.
  18. Josepa, S., J. M. Guillamon, and J. Cano. 2000. PCR differentiation of Saccharomyces cerevisiae from Saccharomyces bayanus/Saccharomyces pastorianus using specific primers. FEMS Microbiol. Lett. 193: 255-259. https://doi.org/10.1111/j.1574-6968.2000.tb09433.x
  19. Kanti, A. and I. Sudiana. 2002. Diversity and ecological perspective of soil yeast in Gunung Halimun National Park. Berita. Biologi. 6: 25-32.
  20. Katiyar, V. and R. Goel. 2003. Solubilization of inorganic phosphate and plant growth promotion by cold tolerant mutants of Pseudomonas fluorescens. Microbiol. Res. 158: 163-168. https://doi.org/10.1078/0944-5013-00188
  21. Kurtzman, C. P. and C. J. Robnett. 1998. Identification and phylogeny of ascomycetous yeasts from analysis of nuclear large subunit (26S) ribosomal DNA partial sequences. Antonie Van Leeuweenhoek 73: 331-371. https://doi.org/10.1023/A:1001761008817
  22. Kurtzman, C. P. 2006. Yeast species recognition from gene sequence analyses and other molecular methods. Mycoscience 47: 65-71. https://doi.org/10.1007/s10267-006-0280-1
  23. Kucey, R. M. N., H. H. Janzen, and M. E. Leggett. 1989. Microbially mediated increases in plant available phosphorus. Adv. Agron. 42: 199-228.
  24. Luo, A. C., X. Sun, and Y. S. Zhang. 1993. Species of inorganic phosphate solubilizing bacteria in red soil and mechanism of solubilization. Pedosphere 3: 285-288.
  25. Mahadevam, A. 1984. Growth Regulators, Microorganisms and Diseased Plants. Oxford & IBH publishing Co., New Dehli.
  26. Narsian, V., S. A. Abu Samaha, and M. Patel. 2008. Rock phosphate dissolution by specific yeast. Ind. J. Microbiol. 50: 57-62.
  27. Omer, S. A. 1998. The role of rock-phosphate-solubilizing fungi and vesicular-arbusular-mycorrhiza (VAM) in growth of wheat plants fertilized with rock phosphate. World J. Microbiol. Biotech. 14: 211-218. https://doi.org/10.1023/A:1008830129262
  28. Pradhan, N. and L. B. Sukla. 2005. Solubilization of inorganic phosphate by fungi isolated from agriculture soil. Afr. J. Biotechnol. 5: 850-854.
  29. Rajankar, P. N., D. H. Tambekar, and S. R. Wate. 2007. Study of phosphate solubilization efficiencies of fungi and bacteria isolated from saline belt of Purna river basin. Res. J. Agric. Sci. 3: 701-703.
  30. Reed, G. and T. W. Nagodawithana. 1991. Yeast Technology, pp. 385-400. 2 Ed. van Nostrand Reinhold, New York.
  31. Scorzetti, G., J. W. Fell, A Fonseca, and A. Statzell-Tallman. 2002. Systematics of basidiomycetous yeasts: A comparison of large subunit D1/D2 and internal transcribed spacer rDNA regions. FEMS Yeast Res. 1497: 1-23.
  32. Sheng, X. F. and W. Y. Huang. 2001. Physiological characteristics of strain NBT of silicate bacterium. Acta Pedol. Sin. 38: 569-574.
  33. Sperber, J. I. 1958. Solution of apatite by soil microorganisms producing organic acid. Austr. J. Agric. Res. 9: 782-789. https://doi.org/10.1071/AR9580782
  34. Stevenson, F. J. 1986. Cycles of Soil Carbon, Nitrogen, Phosphorus, Sulfur, Micronutrients. Wiley, New York.
  35. Sundara Rao, W. V. B. and M. K Sinha. 1963. Phosphate dissolving organisms in the soil and rhizosphere. Ind. J. Agric. Sci. 33: 272-278.
  36. Surange, S. and N. Kumar. 1993. Phosphate solubilization under varying pH by Rhizobium from tree legumes. Ind. J. Expt. Biol. 3: 855-857.
  37. Tisdale, S. L., W. L. Nelson, J. D. Beaton, and J. L. Havlin. 1993. Soil Fertility and Fertilizers, 5th Ed. McMillan Publishing Co., New York.
  38. Vassileva, M., R. Azcon, J. Barea, and N. Vassilev. 2000. Rock phosphate solubilization by free and encapsulated cells of Yarrowia lipolytica. Process Biochem. 35: 693-697. https://doi.org/10.1016/S0032-9592(99)00132-6
  39. Whitelaw, M. A. 2000. Growth promotion of plants inoculated with phosphate solubilizing fungi, pp. 99-151. In Donald L. Sparks (ed.). Advances in Agronomy, Vol. 69. Academic Press.

Cited by

  1. Toxicological assessment of selective pesticides towards plant growth promoting activities of phosphate solubilizing Pseudomonas aeruginosa vol.58, pp.3, 2011, https://doi.org/10.1556/amicr.58.2011.3.1
  2. Phosphate-solubilizing soil yeast Meyerozyma guilliermondii CC1 improves maize (Zea mays L.) productivity and minimizes requisite chemical fertilization vol.373, pp.1, 2011, https://doi.org/10.1007/s11104-013-1792-z
  3. Biocontrol of apple blue mould by new yeast strains: Cryptococcus albidus KKUY0017 and Wickerhamomyces anomalus KKUY0051 and their mode of action vol.24, pp.10, 2011, https://doi.org/10.1080/09583157.2014.926857
  4. Phylogenetic analysis of isolated biofuel yeasts based on 5.8S-ITS rDNA and D1/D2 26S rDNA sequences vol.12, pp.1, 2014, https://doi.org/10.1016/j.jgeb.2014.01.001
  5. Impact of Inoculation with Arbuscular Mycorrhizal, Phosphate Solubilizing Bacteria and Soil Yeast on Growth, Yield and Phosphorus Content of Onion Plants vol.10, pp.2, 2011, https://doi.org/10.3923/ijss.2015.93.99
  6. Preliminary physiological characteristics of thermotolerant Saccharomyces cerevisiae clinical isolates identified by molecular biology techniques vol.62, pp.3, 2011, https://doi.org/10.1111/lam.12542
  7. Effect of Inoculated Azotobacter chroococcum and Soil Yeasts on Growth, N-uptake and Yield of Wheat (Triticum aestivum) under Different Levels of Nitrogen Fertilization vol.11, pp.3, 2016, https://doi.org/10.3923/ijss.2016.102.107
  8. Plant growth promotion and nitrogen fixation in canola (Brassica napus) by an endophytic strain of Paenibacillus polymyxa and its GFP-tagged derivative in a long-term study vol.94, pp.12, 2011, https://doi.org/10.1139/cjb-2016-0075
  9. Identification of Yeast Strains Isolated from Agricultural Soils for Releasing Potassium-bearing Minerals vol.34, pp.3, 2011, https://doi.org/10.1080/01490451.2016.1186762
  10. Molecular Characterization of Fusarium Solani Degrades a Mixture of Low and High Molecular Weight Polycyclic Aromatic Hydrocarbons vol.11, pp.1, 2011, https://doi.org/10.2174/1874070701711010027
  11. Temperature, pH and carbon source affect drastically indole acetic acid production of plant growth promoting yeasts vol.34, pp.2, 2017, https://doi.org/10.1590/0104-6632.20170342s20150541
  12. Feasibility and Sustainability of Bioethanol Production from Starchy restaurants’ Bio-wastes by New Yeast Strains vol.10, pp.6, 2011, https://doi.org/10.1007/s12649-017-0184-7
  13. Yeast a potential bio-agent: future for plant growth and postharvest disease management for sustainable agriculture vol.104, pp.4, 2011, https://doi.org/10.1007/s00253-019-10321-3
  14. Culturable Yeasts as Biofertilizers and Biopesticides for a Sustainable Agriculture: A Comprehensive Review vol.10, pp.5, 2011, https://doi.org/10.3390/plants10050822
  15. Isolation and Identification of Aroma-producing Yeast from Mackerel Fermentation Broth and Its Fermentation Characteristics vol.30, pp.10, 2011, https://doi.org/10.1080/10498850.2021.1988016