Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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A RAPD-PCR Method for the Rapid Detection of Bacillus cereus

  • Lee, Ji-Yeon (Division of Applied Life Science (BK21), Graduate School, Gyeongsang National University) ;
  • Kwon, Gun-Hee (Institute of Agriculture and Life Science, Gyeongsang National University) ;
  • Park, Jae-Yong (Department of Food Science and Nutrition, Catholic University of Daegu) ;
  • Park, Cheon-Seok (Department of Food Science and Technology, Kyunghee University) ;
  • Kwon, Dae-Young (Food Function Research Division, Korea Food Research Institute) ;
  • Lim, Jin-Kyu (Shool of Applied Bioscience, Kyungpook National University) ;
  • Kim, Jong-Sang (Shool of Applied Bioscience, Kyungpook National University) ;
  • Kim, Jeong-Hwan (Division of Applied Life Science (BK21), Graduate School, Gyeongsang National University)
  • Received : 2010.08.25
  • Accepted : 2010.11.17
  • Published : 2011.03.28
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Abstract

Distinction of Bacillus cereus from other closely related bacilli is challenging and new efficient methods are continually demanded. From our previous work on RAPD profiles of bacilli, we found a possibility that B. cereus strains could be distinguished from other bacilli. In this work, RAPD-PCR profiles of B. cereus strains were obtained using a 10-mer (S30) as a primer, and a B. cereus specific 0.91-kb band was produced from all tested strains. The RAPD-PCR procedure also successfully detected B. cereus from spiked cheonggukjang when B. cereus cells were present at more than $10^2$/g sample.

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References

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