DOI QR코드

DOI QR Code

Effects of Pseudomonas aureofaciens 63-28 on Defense Responses in Soybean Plants Infected by Rhizoctonia solani

  • Jung, Woo-Jin (Division of Applied Bioscience and Biotechnology, Institute of Agricultural Science and Technology, Chonnam National University) ;
  • Park, Ro-Dong (Division of Applied Bioscience and Biotechnology, Institute of Agricultural Science and Technology, Chonnam National University) ;
  • Mabood, Fazli (Department of Plant Science, Macdonald Campus of McGill University) ;
  • Souleimanov, Alfred (Department of Plant Science, Macdonald Campus of McGill University) ;
  • Smith, Donald L. (Department of Plant Science, Macdonald Campus of McGill University)
  • Received : 2010.12.02
  • Accepted : 2011.01.04
  • Published : 2011.04.28

Abstract

The objective of this work was to investigate the ability of the plant growth-promoting rhizobacterium Pseudomonas aureofaciens 63-28 to induce plant defense systems, including defense-related enzyme levels and expression of defense-related isoenzymes, and isoflavone production, leading to improved resistance to the phytopathogen Rhizoctonia solani AG-4 in soybean seedlings. Seven-day-old soybean seedlings were inoculated with P. aureofaciens 63-28, R. solani AG-4, or P. aureofaciens 63-28 plus R. solani AG-4 (P+R), or not inoculated (control). After 7 days of incubation, roots treated with R. solani AG-4 had obvious damping-off symptoms, but P+R-treated soybean plants had less disease development, indicating suppression of R. solani AG-4 in soybean seedlings. Superoxide dismutase (SOD) and catalase (CAT) activities of R. solani AG-4-treated roots increased by 24.6% and 54.0%, respectively, compared with control roots. Ascorbate peroxidase (APX) and phenylalanine ammonia lyase (PAL) activities of R. solani AG-4-treated roots were increased by 75.1% and 23.6%, respectively. Polyphenol oxidase (PPO) activity in soybean roots challenged with P. aureofaciens 63-28 and P+R increased by 25.0% and 11.6%, respectively. Mn-SOD (S1 band on gel) and Fe-SOD (S2) were strongly induced in P+R-treated roots, whereas one CAT (C1) and one APX (A3) were strongly induced in R. solani AG-4- treated roots. The total isoflavone concentration in P+Rtreated shoots was 27.2% greater than the control treatment. The isoflavone yield of R. solani AG-4-treated shoots was 60.9% less than the control.

Keywords

References

  1. Atlas, R. M. 1995. Handbook of Media for Environmental Microbiology. CRC Press., Boca Raton, FL.
  2. Beaudoin-Eagan, L. D. and T. A. Thorpe. 1985. Tyrosine and phenylalanine ammonia lyase activities during shoot initiation in tobacco callus cultures. Plant Physiol. 78: 438-441. https://doi.org/10.1104/pp.78.3.438
  3. Bradford, M. M. 1976. A rapid sensitive method for the quantification of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72: 248-254. https://doi.org/10.1016/0003-2697(76)90527-3
  4. Chance, B. and A. C. Maehly. 1955 Assay of catalase and peroxidase, pp. 764-775. In S. P. Colowick and N. O. Kaplan (eds.). Methods in Enzymology. Academic Press, New York.
  5. Chen, G. and K. Asada. 1989. Ascorbate peroxidase in tea leaves: Occurrence of two isozymes and the differences in their enzymatic and molecular properties. Plant Cell Physiol. 30: 987-998.
  6. Chen, C., R. R. Bélanger, N. Benhamou, and T. C. Paulitz. 2000. Defense enzymes induced in cucumber roots by treatment with plant growth-promoting rhizobacteria (PGPR) and Pythium aphanidermatum. Physiol. Mol. Plant Pathol. 56: 13-23. https://doi.org/10.1006/pmpp.1999.0243
  7. Fridovich, I. 1989. Superoxide dismutase. An adaptation to a paramagnetic gas. J. Biol. Chem. 264: 7761-7764.
  8. Gamard, P., F. Sauriol, N. Benhamou, R. R. Belanger, and T. C. Paulitz. 1997. Novel butyrolactones with antifungal activity produced by Pseudomonas aureofaciens strain 63-28. J. Antibiot. 50: 742-749. https://doi.org/10.7164/antibiotics.50.742
  9. Giannopolitis, C. N. and S. K. Ries. 1977. Superoxide dismutases: I. Occurrence in higher plants. Plant Physiol. 59: 309-314. https://doi.org/10.1104/pp.59.2.309
  10. Graham, M. Y. and T. L. Graham. 1994. Wound-associated competency factors are required for the proximal cell responses of soybean to the Phytophthora sojae wall glucan elicitor. Plant Physiol. 105: 571-578.
  11. Howell, C. R. and R. D. Stipanovic. 1980. Suppression of Pythium ultimum induced damping-off of cotton seedlings by Pseudomonas fluorescens and its antibiotic pyoluteorin. Phytopathology 70: 712-715 https://doi.org/10.1094/Phyto-70-712
  12. Jung, W. J., Y. L. Jin, Y. C. Kim, K. Y. Kim, R. D. Park, and T. H. Kim. 2004. Inoculation of Paenibacillus illinoisensis alleviates root mortality, activates of lignification-related enzymes, and induction of the isozymes in pepper plants infected by Phytophthora capsici. Biol. Control 30: 645-652. https://doi.org/10.1016/j.biocontrol.2004.03.006
  13. Kim, B. S., S. S. Moon, and B. K. Hwang. 1999. Isolation, identification and antifungal activity of a macrolide antibiotic, oligomycin A, produced by Streptomyces libani. Can. J. Bot. 77: 850-858.
  14. Kramer, R. P., H. Hindorf, H. C. Jha, J. Kallage, and F. Zilliken. 1984. Antifungal activity of soybean and chickpea isoflavones and their reduced derivatives. Phytochemistry 23: 2203-2205. https://doi.org/10.1016/S0031-9422(00)80520-8
  15. Kristensen, B. K., H. Bloch, and S. K. Rasmussen. 1999. Barley coleoptile peroxidases: Purification, molecular cloning, and induction by pathogens. Plant Physiol. 120: 501-512. https://doi.org/10.1104/pp.120.2.501
  16. Maurhofer, M., C. Hase, P. Meuwly, J. P. Metraux, and G. Defago. 1994. Induction of systemic resistance of tobacco to tobacco necrosis virus by the root-colonizing Pseudomonas fluorescens strain CHAO: Influence of the gacA gene and of pyoverdine production. Phytopathology 84: 139-146. https://doi.org/10.1094/Phyto-84-139
  17. Mellon, J. E. and L. S. Lee. 1985. Elicitation of cotton isoperoxidases by Aspergillus flavus and other fungi pathogenic to cotton. Physiol. Plant Pathol. 27: 281-288. https://doi.org/10.1016/0048-4059(85)90041-4
  18. Milner, J. L., S. J. Raffel, B. J. Lethbridge, and J. Handelsman. 1995. Culture conditions that influence accumulation of zwittermicin A by Bacillus cereus UW85. Appl. Microbiol. Biotechnol. 43: 685-691. https://doi.org/10.1007/BF00164774
  19. Mohammadi, M. and H. Kazemi. 2002. Changes in peroxidase and polyphenol oxidase activities in susceptible and resistant wheat heads inoculated with Fusarium graminearum and induced resistance. Plant Sci. 162: 491-498. https://doi.org/10.1016/S0168-9452(01)00538-6
  20. Morris, P. F., M. E. Savard, and E. W. B. Ward. 1991. Identification and accumulation of isoflavonoids and isoflavone glucosides in soybean leaves and hypocotyls in resistance responses to Phytophthora megasperma f. sp. glycinea. Physiol. Mol. Plant Pathol. 39: 229-244. https://doi.org/10.1016/0885-5765(91)90006-4
  21. Nakayama, T., Y. Homma, Y. Hashidoko, J. Mizutani, and S. Tahara. 1999. Possible role of xanthobaccins produced by Stenotrophomonas sp. strain SB-K88 in suppression of sugar beet damping-off disease. Appl. Environ. Microbiol. 65: 4334- 4339.
  22. Petterson, H. and K. H. Kiessling. 1984. Liquid chromatographic determination of the plant estrogens coumestrol and isoflavones in animal feed. J. Assoc. Off. Anal. Chem. 67: 503-506.
  23. Racchi, M. L., F. Bagnoli, I. Balla, and S. Danti. 2001. Differential activity of catalase and superoxide dismutase in seedlings and in vitro micropropagated oak (Quercus robur L.). Plant Cell Rep. 20: 169-174. https://doi.org/10.1007/s002990000300
  24. Ramamoorthy, V., T. Raguchander, and R. Samiyappan. 2002. Induction of defense-related proteins in tomato roots treated with Pseudomonas fluorescens Pf1 and Fusarium oxysporum f. sp. lycopersici. Plant Soil 239: 55-68. https://doi.org/10.1023/A:1014904815352
  25. Ramanathan, A., R. Samiyappan, and P. Vidyasekaran. 2000. Induction of defence mechanisms in greengram leaves and suspension cultured cells by Macrophomina phaseolina and its elicitors. J. Plant Dis. Protect. 107: 245-257.
  26. Rao, M. V., G. Paliyath, and D. P. Ormrod. 1996. Ultraviolet-Band ozone-induced biochemical changes in antioxidant enzymes of Arabidopsis thaliana. Plant Physiol. 110: 125-136. https://doi.org/10.1104/pp.110.1.125
  27. Ray, H., D. S. Douches, and R. Hammerschmidt. 1998. Transformation of potato with cucumber peroxidase: Expression and disease response. Physiol. Mol. Plant Pathol. 53: 93-103. https://doi.org/10.1006/pmpp.1998.0164
  28. Rivera-Vargas, L. I., A. F. Schmitthenner, and T. L. Graham. 1993. Soybean flavonoid effects on and metabolism by Phytophthora sojae. Phytochemistry 32: 851-857. https://doi.org/10.1016/0031-9422(93)85219-H
  29. Ryals, J. A., U. H. Neuenschwander, M. G. Willits, A. Molina, H. Y. Steiner, and M. D. Hunt. 1996. Systemic acquired resistance. Plant Cell 8: 1809-1819.
  30. SAS institute. 2002. SAS/STAT User's Guide, Version 9.1. Cary, NC, USA.
  31. Siriphanich, J. and A. A. Kader. 1985. Effects of $CO_2$on cinnamic acid-4-hydroxylase in relation to phenolic metabolism in lettuce tissue. J. Am. Soc. Hortic. Sci. 110: 333-335.
  32. Sobkowiak, R., K. Rymer, R. Rucinska, and J. Deckert. 2004. Cadmium-induced changes in antioxidant enzymes in suspension culture of soybean cells. Acta Biochim. Pol. 51: 219-222.
  33. Wei, G., J. W. Kloepper, and S. Tuzun. 1991. Induction of systemic resistance of cucumber to Colletotrichum orbiculare by select strains of plant growth-promoting rhizobacteria. Phytopathology 81: 221-224.
  34. Whipps, J. M. 2001. Microbial interactions and biocontrol in the rhizosphere. J. Exp. Bot. 52: 487-511. https://doi.org/10.1093/jexbot/52.suppl_1.487
  35. Xue, L., P. M. Charest, and S. H. Jabaji-Hare. 1998. Systemic induction of peroxidases, 1,3-b-glucanases, chitinases, and resistance in bean plants by binucleate Rhizoctonia species. Phytopathology 88: 359-365. https://doi.org/10.1094/PHYTO.1998.88.4.359
  36. Yoshimura, K., Y. Yabuta, T. Ishikawa, and S. Shigeoka. 2000. Expression of spinach ascorbate peroxidase isoenzymes in response to oxidative stresses. Plant Physiol. 123: 223-233. https://doi.org/10.1104/pp.123.1.223
  37. Zhou, T. and T. C. Paulitz. 1994. Induced resistance in the biological control of Pythium aphanidermatum by Pseudomonas spp. on European cucumber. J. Phytopathol. 142: 51-63.

Cited by

  1. Foliar application of Burkholderia sp. strain TNAU-1 leads to activation of defense responses in chilli (Capsicum annuum L.) vol.23, pp.4, 2011, https://doi.org/10.1590/s1677-04202011000400003
  2. Induction of Defense Response Against Rhizoctonia solani in Cucumber Plants by Endophytic Bacterium Bacillus thuringiensis GS1 vol.22, pp.3, 2011, https://doi.org/10.4014/jmb.1107.07027
  3. Characterization of Mycolytic Enzymes of Bacillus Strains and Their Bio-Protection Role Against Rhizoctonia solani in Tomato vol.65, pp.3, 2011, https://doi.org/10.1007/s00284-012-0160-1
  4. Investigating binucleateRhizoctoniainduced defence responses in kidney bean againstRhizoctonia solani vol.25, pp.4, 2015, https://doi.org/10.1080/09583157.2014.984285
  5. Characterization of antagonistic‐potential of two Bacillus strains and their biocontrol activity against Rhizoctonia solani in tomato vol.55, pp.1, 2011, https://doi.org/10.1002/jobm.201300528
  6. Co-inoculation of different antagonists can enhance the biocontrol activity against Rhizoctonia solani in tomato vol.112, pp.11, 2019, https://doi.org/10.1007/s10482-019-01290-8
  7. Proteomic profiling uncovered the cytosolic superoxide dismutase BsSOD1 associated with plant defence in the herbal orchid Bletilla striata vol.47, pp.10, 2011, https://doi.org/10.1071/fp19345