Journal of Microbiology and Biotechnology

  • 제21권5호
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  • Pages.515-518
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  • 2011
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  • 1017-7825(pISSN)
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  • 1738-8872(eISSN)
한국미생물·생명공학회 (The Korean Society for Microbiology and Biotechnology)
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Cloning and Expression of a bpr Gene Encoding Bacillopeptidase F from Bacillus amyloliquefaciens CH86-1

  • Kwon, Gun-Hee (Division of Applied Life Science (BK21 Program), Graduate School, Gyeongsang National University) ;
  • Park, Jae-Yong (Department of Food Science and Nutrition, Catholic University of Daegu) ;
  • Kim, Jong-Sang (School of Applied Biosciences, Kyungpook National University) ;
  • Lim, Jin-Kyu (School of Applied Biosciences, Kyungpook National University) ;
  • Park, Cheon-Seok (Department of Food Science and Biotechnology, Kyung Hee University) ;
  • Kwon, Dae-Young (Food Function Research Division, Korea Food Research Institute) ;
  • Kim, Jeong-Hwan (Division of Applied Life Science (BK21 Program), Graduate School, Gyeongsang National University)
  • 투고 : 2010.10.27
  • 심사 : 2011.02.12
  • 발행 : 2011.05.28
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초록

A gene encoding bacillopeptidase F, bpr86-1, was cloned from B. amyloliquefaciens CH86-1 isolated from cheonggukjang. This gene could encode a preproenzyme of 1,431 amino acids. When bpr86-1 was introduced into B. subtilis WB600 via pHY300PLK, an E. coli-Bacillus shuttle vector, the transformant showed fibrinolytic activity. During growth on LB, the fibrinolytic activity of cells increased sharply when they entered the stationary phase. The highest activity (761.4 mU/mg protein) was observed at 96 h of cultivation.

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참고문헌

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피인용 문헌

  1. Maturation of Fibrinolytic Bacillopeptidase F Involves both Hetero- and Autocatalytic Processes vol.82, pp.1, 2011, https://doi.org/10.1128/aem.02673-15
  2. Current Perspectives on the Physiological Activities of Fermented Soybean-Derived Cheonggukjang vol.22, pp.11, 2011, https://doi.org/10.3390/ijms22115746
  3. Zymogram Analysis and Identification of the Extracellular Proteases from Bacillus velezensis SW5 vol.57, pp.suppl1, 2021, https://doi.org/10.1134/s0003683821100082