DOI QR코드

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Coenzyme $Q_{10}$ Production by Sphingomonas sp. ZUTE03 with Novel Precursors Isolated from Tobacco Waste in a Two-Phase Conversion System

  • Qiu, Lequan (College of Biological and Environmental Engineering, Zhejiang University of Technology) ;
  • Wang, Weijian (College of Biological and Environmental Engineering, Zhejiang University of Technology) ;
  • Zhong, Weihong (College of Biological and Environmental Engineering, Zhejiang University of Technology) ;
  • Zhong, Li (College of Biological and Environmental Engineering, Zhejiang University of Technology) ;
  • Fang, Jianjun (College of Biological and Environmental Engineering, Zhejiang University of Technology) ;
  • Li, Xuanzhen (College of Biological and Environmental Engineering, Zhejiang University of Technology) ;
  • Wu, Shijin (College of Biological and Environmental Engineering, Zhejiang University of Technology) ;
  • Chen, Jianmeng (College of Biological and Environmental Engineering, Zhejiang University of Technology)
  • 투고 : 2010.12.13
  • 심사 : 2011.01.31
  • 발행 : 2011.05.28

초록

Coenzyme $Q_{10}$ ($CoQ_{10}$) is a widely used supplement in heart diseases treatment or antioxidative dietary. The microbial production of $CoQ_{10}$ was enhanced by addition of solanesol and novel precursors recovered from waste tobacco. The novel precursors were separated by silica gel and identified as ${\alpha}$-linolenic acid (LNA) and butylated hydroxytoluene (BHT) based on the effect on $CoQ_{10}$ production and GC-MS. The effects of novel precursors on $CoQ_{10}$ production by Sphingomonas sp. ZUTE03 were further evaluated in a two-phase conversion system. The precursor's combination of solanesol (70 mg/l) with BHT (30 mg/l) showed the best effect on the improvement of $CoQ_{10}$ yield. A maximal $CoQ_{10}$ productivity (9.5 mg $l^{-1}$ $h^{-1}$) was achieved after 8 h conversion, with a molar conversion rate of 92.6% and 92.4% on BHT and solanesol, respectively. The novel precursors, BHT and LNA in crude extracts from waste tobacco leaves, might become potential candidates for application in the industrial production of $CoQ_{10}$ by microbes.

키워드

참고문헌

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  2. Cellular factories for coenzyme Q 10 production vol.16, pp.None, 2017, https://doi.org/10.1186/s12934-017-0646-4
  3. Production of coenzyme Q10 by purple non-sulfur bacteria: Current development and future prospect vol.307, pp.None, 2011, https://doi.org/10.1016/j.jclepro.2021.127326