Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Ferric Reductase Activity of the ArsH Protein from Acidithiobacillus ferrooxidans

  • Mo, Hongyu (College of Biology, Hunan University) ;
  • Chen, Qian (College of Biology, Hunan University) ;
  • Du, Juan (College of Biology, Hunan University) ;
  • Tang, Lin (College of Biology, Hunan University) ;
  • Qin, Fang (College of Biology, Hunan University) ;
  • Miao, Bo (Department of Bioengineering, Central South University) ;
  • Wu, Xueling (Department of Bioengineering, Central South University) ;
  • Zeng, Jia (College of Biology, Hunan University)
  • Received : 2011.01.14
  • Accepted : 2011.03.11
  • Published : 2011.05.28
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Abstract

The arsH gene is one of the arsenic resistance system in bacteria and eukaryotes. The ArsH protein was annotated as a NADPH-dependent flavin mononucleotide (FMN) reductase with unknown biological function. Here we report for the first time that the ArsH protein showed high ferric reductase activity. Glu104 was an essential residue for maintaining the stability of the FMN cofactor. The ArsH protein may perform an important role for cytosolic ferric iron assimilation in vivo.

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References

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