Journal of Microbiology and Biotechnology

  • 제21권6호
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  • Pages.567-573
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  • 2011
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  • 1017-7825(pISSN)
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  • 1738-8872(eISSN)
한국미생물·생명공학회 (The Korean Society for Microbiology and Biotechnology)
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The Phylogenetic Affiliation of an Uncultured Population of Ammonia-Oxidizing Bacteria Harboring Environmental Sequences of amoA Cluster-3

  • Hong, Jin-Kyung (Institute of Environmental Sciences and Department of Environmental Sciences, Hankuk University of Foreign Studies) ;
  • Cho, Jae-Chang (Institute of Environmental Sciences and Department of Environmental Sciences, Hankuk University of Foreign Studie)
  • 투고 : 2011.01.17
  • 심사 : 2011.03.23
  • 발행 : 2011.06.28
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초록

We investigated the phylogenetic diversity of ammoniaoxidizing bacteria (AOB) in Yellow Sea continental shelf sediment by the cloning and sequencing of PCR-amplified amoA and 16S rRNA genes. Phylogenetic analysis of the amoA-related clones revealed that the diversity of AOB was extremely low at the study site. The majority (92.7%) of amoA clones obtained belonged to a single cluster, environmental amoA cluster-3, the taxonomic position of which was previously unknown. Phylogenetic analysis on AOB-specific 16S rRNA gene sequences also demonstrated a very low diversity. All of the cloned 16S rRNA gene sequences comprised a single phylotype that belonged to the members of uncultured Nitrosospira cluster-1, suggesting that AOB belonging to the uncultured Nitrosospira cluster-1 could carry amoA sequences of environmental amoA cluster-3.

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