Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Improvement of Transformation Efficiency by Strategic Circumvention of Restriction Barriers in Streptomyces griseus

  • Suzuki, Hirokazu (Chemical Biology Department, Advanced Science Institute, RIKEN) ;
  • Takahashi, Shunji (Chemical Biology Department, Advanced Science Institute, RIKEN) ;
  • Osada, Hiroyuki (Chemical Biology Department, Advanced Science Institute, RIKEN) ;
  • Yoshida, Ken-Ichi (Department of Agrobioscience, Graduate School of Agricultural Science, Kobe University)
  • Received : 2011.02.28
  • Accepted : 2011.04.13
  • Published : 2011.07.28
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Abstract

DNA methylation in Streptomyces griseus IFO 13350 was analyzed by high-performance liquid chromatographic analysis and bisulfite-based analysis to reveal two methylation sites, 5'-$GC^{5m}$ CGGC-3' and 5'-$GAG^{5m}$ CTC-3'. The methylation was reconstituted in Escherichia coli by simultaneous expression of S. griseus SGR4675 and S. achromogenes M.SacI. The E. coli cells produced plasmids that mimicked the methylation profile of S. griseus DNA, which was readily introduced into S. griseus. The results of this study raise the possibility of a promising approach to establish efficient transformation in several streptomycetes.

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References

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