Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Production of 1,2-Propanediol from Glycerol in Saccharomyces cerevisiae

  • Jung, Joon-Young (Department of Chemical and Biological Engineering, Korea University) ;
  • Yun, Hyun-Shik (Department of Biotechnology, Inha University) ;
  • Lee, Jin-Won (Department of Chemical and Biomolecular Engineering, Sogang University) ;
  • Oh, Min-Kyu (Department of Chemical and Biological Engineering, Korea University)
  • Received : 2011.03.04
  • Accepted : 2011.04.28
  • Published : 2011.08.28
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Abstract

Glycerol has become an attractive carbon source in the biotechnology industry owing to its low price and reduced state. However, glycerol is rarely used as a carbon source in Saccharomyces cerevisiae because of its low utilization rate. In this study, we used glycerol as a main carbon source in S. cerevisiae to produce 1,2-propanediol. Metabolically engineered S. cerevisiae strains with overexpression of glycerol dissimilation pathway genes, including glycerol kinase (GUT1), glycerol 3-phosphate dehydrogenase (GUT2), glycerol dehydrogenase (gdh), and a glycerol transporter gene (GUP1), showed increased glycerol utilization and growth rate. More significant improvement of glycerol utilization and growth rate was accomplished by introducing 1,2-propanediol pathway genes, mgs (methylglyoxal synthase) and gldA (glycerol dehydrogenase) from Escherichia coli. By engineering both glycerol dissimilation and 1,2-propanediol pathways, the glycerol utilization and growth rate were improved 141% and 77%, respectively, and a 2.19 g 1,2- propanediol/l titer was achieved in 1% (v/v) glycerolcontaining YEPD medium in engineered S. cerevisiae.

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References

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