Journal of Microbiology and Biotechnology

  • 제21권8호
  • /
  • Pages.818-821
  • /
  • 2011
  • /
  • 1017-7825(pISSN)
  • /
  • 1738-8872(eISSN)
한국미생물·생명공학회 (The Korean Society for Microbiology and Biotechnology)
권호 표지
DOI QR코드

DOI QR Code

Isolation and Characterization of a Novel Agarase-Producing Pseudoalteromonas spp. Bacterium from the Guts of Spiny Turban Shells

  • Oh, Young-Hoon (Department of Chemical and Biomolecular Engineering, Sogang University) ;
  • Jung, Chang-Kyou (Department of Chemical and Biomolecular Engineering, Sogang University) ;
  • Lee, Jin-Won (Department of Chemical and Biomolecular Engineering, Sogang University)
  • 투고 : 2010.12.24
  • 심사 : 2011.05.25
  • 발행 : 2011.08.28
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

An agar-degrading bacterium was isolated from the guts of spiny turban shells. It was identified as a Pseudoalteromonas species and named Pseudoalteromonas sp. JYBCL 1. The viscosity of the inoculated agar medium decreased by more than 60% after 20 h cultivation. The agarase produced by the isolate had optimal activities at $35^{\circ}C$ and pH 7. The enzyme had extremely strong resistance to ionic stress compared with other known agarases. Its molecular mass was estimated at about 60 kDa by sodium dodecyl sulfate polyacrylamide gel electrophoresis. The agarase could saccharify Gelidium amansii directly, with an efficiency about half that compared with agar saccharification.

키워드

참고문헌

  1. Araki, T., M. Hayakawa, L. Zhang, S. Karita, and T. Morishita. 1998. Purification and characterization of agarases from a marine bacterium, Vibrio sp. PO-303. J. Mar. Biotechnol. 6: 260-265.
  2. Belas, R. 1989. Sequence analysis of the agrA gene encoding $\beta$- agarase from Pseudomonas atlantica. J. Bacteriol. 171: 602- 605. https://doi.org/10.1128/jb.171.1.602-605.1989
  3. Chiura, H. and K. Tsukamoto. 2000. Purification and characterisation of novel agarase secreted by marine bacterium, Pseudoalteromonas sp. strain CKT1. Microbes Environ. 15: 11-22. https://doi.org/10.1264/jsme2.2000.11
  4. Duckworth, M. and J. R. Turvey. 1969. An extracellular agarase from a Cytophaga species. Biochem. J. 113: 139-142. https://doi.org/10.1042/bj1130139
  5. Fu, X. T., H. Lin, and S. M. Kim. 2008. Purification and characterization of a novel $\beta$-agarase, AgaA34, from Agarivorans albus YKW-34. Appl. Microbiol. Biotechnol. 78: 265-273. https://doi.org/10.1007/s00253-007-1303-3
  6. Ha, J. C., G. T. Kim, S. K. Kim, T. K. Oh, J. H. Yu, and I. S. Kong. 1997. Beta-agarase from Pseudomonas sp. W7: Purification of the recombinant enzyme from Escherichia coli and the effects of salt on its activity. Biotechnol. Appl. Biochem. 26: 1-6.
  7. Ha, J. C., G. T. Kim, S. K. Kim, T. K. Oh, J. H. Yu, and I. S. Kong. 1997. Beta-agarase from Pseudomonas sp. W7: Purification of the recombinant enzyme from Escherichia coli and the effects of salt on its activity. Biotechnol. Appl. Biochem. 26: 1-6.
  8. Hu, Z., B. K. Lin, Y. Xu, M. Q. Zhong, and G. M. Liu. 2008. Production and purification of agarase from a marine agarolytic bacterium Agarivorans sp. HZ105. J. Appl. Microbiol. 106: 181-190.
  9. Kang, N. Y., Y. L. Choi, Y. S. Cho, B. K. Kim, B. S. Jeon, J. Y. Cha, C. H. Kim, and Y. C. Lee. 2003. Cloning, expression and characterization of a beta-agarase gene from a marine bacterium, Pseudomonas sp. SK38. Biotechnol. Lett. 25: 1165-1170. https://doi.org/10.1023/A:1024586207392
  10. Kirimura, K., N. Masuda, Y. Iwasaki, H. Nakagawa, R. Kobayashi, and S. Usami. 1999. Purification and characterization of a novel $\beta$-agarase from an alkalophilic bacterium, Alteromonas sp. E-1. J. Biosci. Bioeng. 87: 436-441. https://doi.org/10.1016/S1389-1723(99)80091-7
  11. Lakshmikanth, M., S. Manohar, Y. Souche, and J. Lalitha. 2006. Extracellular $\beta$-agarase LSL-1 producing neoagarobiose from a newly isolated agar-liquefying soil bacterium, Acinetobacter sp., AG LSL-1. World J. Microbiol. Biotechnol. 22: 1087-1094. https://doi.org/10.1007/s11274-006-9147-z
  12. Leon, O., L. Quintana, G. Peruzzo, and J. C. Slebe. 1992. Purification and properties of an extracellular agarase from Alteromonas sp. strain C-1. Appl. Environ. Microbiol. 58: 4060-4063.
  13. Ma, C., X. Lu, C. Shi, J. Li, Y. Gu, Y. Ma, et al. 2007. Molecular cloning and characterization of a novel beta-agarase, AgaB, from marine Pseudoalteromonas sp. CY24. J. Biol. Chem. 282: 3747-3754.
  14. Morrice, L. M., M. W. McLean, W. F. Long, and F. B. Williamson. 1983. Beta-agarases I and II from Pseudomonas atlantica. Substrate specificities. Eur. J. Biochem. 137: 149-154. https://doi.org/10.1111/j.1432-1033.1983.tb07808.x
  15. Morrice, L. M., M. W. McLean, F. B. Williamson, and W. F. Long. 1983. Beta-agarases I and II from Pseudomonas atlantica. Purifications and some properties. Eur. J. Biochem. 135: 553- 558. https://doi.org/10.1111/j.1432-1033.1983.tb07688.x
  16. Oh, C., C. Nikapitiya, Y. Lee, I. Whang, D. H. Kang, S. J. Heo, Y. U. Choi, and J. Lee. 2010. Molecular cloning, characterization and enzymatic properties of a novel ${\beta}--agarase$ from a marine isolate Pseudoalteromonas sp. AG52. Braz. J. Microbiol. 41: 876-889. https://doi.org/10.1590/S1517-83822010000400006
  17. Oh, C., C. Nikapitiya, Y. Lee, I. Whang, S. J. Kim, D. H. Kang, and J. Lee. 2010. Cloning, purification and biochemical characterization of beta agarase from the marine bacterium Pseudoalteromonas sp. AG4. J. Ind. Biotechnol. 37: 483-494. https://doi.org/10.1007/s10295-010-0694-9
  18. Potin, P., C. Richard, C. Rochas, and B. Kloareg. 1993. Purification and characterization of the alpha-agarase from Alteromonas agarlyticus (Cataldi) comb. nov., strain GJ1B. Eur. J. Biochem. 214: 599-607. https://doi.org/10.1111/j.1432-1033.1993.tb17959.x
  19. Rhee, Y. J., R. H. Cho, W. C. Kim, D. Y. Jun, I. K. Rhee, and Y. H. Kim. 2010. Isolation of a novel freshwater agarolytic Cellvibrio sp. KY-YJ-3 and characterization of its extracellular $\beta$-agarase. J. Microbiol. Biotechnol. 20: 1378-1385. https://doi.org/10.4014/jmb.1007.07010
  20. Schroeder, D. C., M. A. Jaffer, and V. E. Coyne. 2003. Investigation of the role of a beta(1-4) agarase produced by Pseudoalteromonas gracilis B9 in eliciting disease symptoms in the red alga Gracilaria gracilis. Microbiology. 149: 2919-2929. https://doi.org/10.1099/mic.0.26513-0
  21. Sugano, Y., I. Terada, M. Arita, and M. Noma. 1993. Purification and characterization of a new agarase from a marine bacterium, Vibrio sp. strain JT0107. Appl. Environ. Microbiol. 59: 1549- 1554.
  22. Suzuki, H., Y. Sawai, T. Suzuki, and K. Kawai. 2002. Purification and characterization of an extracellular a-neoagarooligosaccharide hydrolase from BaciZllus sp. MK03. J. Biosci. Bioeng. 93: 456-463. https://doi.org/10.1016/S1389-1723(02)80092-5
  23. Turvey, J. R. and J. Christison. 1967. The hydrolysis of algal galactans by enzymes from a Cytophaga species. Biochem. J. 105: 311-316. https://doi.org/10.1042/bj1050311
  24. Wang, J., H. Mou, X. Jiang, and H. Guan. 2006. Characterization of ${\beta}--agarase$ from marine Alteromonas sp. SY37-12 and its degrading products. Appl. Microbiol. Biotechnol. 71: 833-839. https://doi.org/10.1007/s00253-005-0207-3
  25. Van der Meulen, H. J. and W. Harder. 1975. Production and characterization of the agarase of Cytophaga flevensis. Antonie. Van Leeuwenhoek 41: 431-447. https://doi.org/10.1007/BF02565087
  26. Vera, J., R. Alvarez, E. Murano, J. C. Slebe, and O. Leon. 1998. Identification of a marine agarolytic Pseudoalteromonas isolate and characterization of its extracellular agarase. Appl. Environ. Microbiol. 64: 4378-4383.

피인용 문헌

  1. Optimization of Pseudoalteromonas sp. JYBCL 1 Culture Conditions, Medium Composition and Extracellular ${\beta}$-agarase Activity vol.17, pp.5, 2011, https://doi.org/10.1007/s12257-012-0009-2
  2. Agarase의 분류, 기원, 확보, 활성파악, 분리정제, 생산 및 응용 vol.22, pp.2, 2011, https://doi.org/10.5352/jls.2012.22.2.266
  3. Complete genome sequence of marine bacterium Pseudoalteromonas phenolica bacteriophage TW1 vol.159, pp.1, 2011, https://doi.org/10.1007/s00705-013-1776-6
  4. 서해안 해수로부터 분리한 한천분해 해양미생물 Pseudoalteromonas sp. H9의 동정 및 특성 연구 vol.43, pp.2, 2011, https://doi.org/10.4014/mbl.1503.03003
  5. 해양성 Marinomonas sp. SH-2 균주가 생성하는 agarase의 분리 및 특성조사 vol.26, pp.2, 2016, https://doi.org/10.5352/jls.2016.26.2.198
  6. 한천분해세균 Agarivorans sp. KC-1의 분리 및 내열성 β-아가라제의 특성 규명 vol.28, pp.9, 2018, https://doi.org/10.5352/jls.2018.28.9.1056
  7. 해양세균 Agarivorans sp. BK-1의 분리 및 β-아가라제의 특성 규명 vol.29, pp.11, 2011, https://doi.org/10.5352/jls.2019.29.11.1173