Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Isolation and Characterization of a Novel Agarase-Producing Pseudoalteromonas spp. Bacterium from the Guts of Spiny Turban Shells

  • Oh, Young-Hoon (Department of Chemical and Biomolecular Engineering, Sogang University) ;
  • Jung, Chang-Kyou (Department of Chemical and Biomolecular Engineering, Sogang University) ;
  • Lee, Jin-Won (Department of Chemical and Biomolecular Engineering, Sogang University)
  • Received : 2010.12.24
  • Accepted : 2011.05.25
  • Published : 2011.08.28
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Abstract

An agar-degrading bacterium was isolated from the guts of spiny turban shells. It was identified as a Pseudoalteromonas species and named Pseudoalteromonas sp. JYBCL 1. The viscosity of the inoculated agar medium decreased by more than 60% after 20 h cultivation. The agarase produced by the isolate had optimal activities at $35^{\circ}C$ and pH 7. The enzyme had extremely strong resistance to ionic stress compared with other known agarases. Its molecular mass was estimated at about 60 kDa by sodium dodecyl sulfate polyacrylamide gel electrophoresis. The agarase could saccharify Gelidium amansii directly, with an efficiency about half that compared with agar saccharification.

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References

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