Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Salinity Stress Resistance Offered by Endophytic Fungal Interaction Between Penicillium minioluteum LHL09 and Glycine max. L

  • Khan, Abdul Latif (School of Applied Biosciences, College of Agriculture and Life Sciences, Kyungpook National University) ;
  • Hamayun, Muhammad (Department of Botany, Abdul Wali Khan University) ;
  • Ahmad, Nadeem (Department of Botany, Islamia College University) ;
  • Hussain, Javid (Kohat University of Science and Technology) ;
  • Kang, Sang-Mo (School of Applied Biosciences, College of Agriculture and Life Sciences, Kyungpook National University) ;
  • Kim, Yoon-Ha (School of Applied Biosciences, College of Agriculture and Life Sciences, Kyungpook National University) ;
  • Adnan, Muhammad (Kohat University of Science and Technology) ;
  • Tang, Dong-Sheng (Key Laboratory of Agri-biodiversity and Pest Management, Yunnan Agricultural University) ;
  • Waqas, Muhammad (School of Applied Biosciences, College of Agriculture and Life Sciences, Kyungpook National University) ;
  • Radhakrishnan, Ramalingam (School of Applied Biosciences, College of Agriculture and Life Sciences, Kyungpook National University) ;
  • Hwang, Young-Hyun (School of Applied Biosciences, College of Agriculture and Life Sciences, Kyungpook National University) ;
  • Lee, In-Jung (School of Applied Biosciences, College of Agriculture and Life Sciences, Kyungpook National University)
  • Received : 2011.03.07
  • Accepted : 2011.06.02
  • Published : 2011.09.28
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Abstract

Endophytic fungi are little known for their role in gibberellins (GAs) synthesis and abiotic stress resistance in crop plants. We isolated 10 endophytes from the roots of field-grown soybean and screened their culture filtrates (CF) on the GAs biosynthesis mutant rice line - Waito-C. CF bioassay showed that endophyte GMH-1B significantly promoted the growth of Waito-C compared with controls. GMH-1B was identified as Penicillium minioluteum LHL09 on the basis of ITS regions rDNA sequence homology and phylogenetic analyses. GC/MS-SIM analysis of CF of P. minioluteum revealed the presence of bioactive $GA_4$ and $GA_7$. In endophyte-soybean plant interaction, P. minioluteum association significantly promoted growth characteristics (shoot length, shoot fresh and dry biomasses, chlorophyll content, and leaf area) and nitrogen assimilation, with and without sodium chloride (NaCl)-induced salinity (70 and 140 mM) stress, as compared with control. Field-emission scanning electron microcopy showed active colonization of endophyte with host plants before and after stress treatments. In response to salinity stress, low endogenous abscisic acid and high salicylic acid accumulation in endophyte-associated plants elucidated the stress mitigation by P. minioluteum. The endophytic fungal symbiosis of P. minioluteum also increased the daidzein and genistein contents in the soybean as compared with control plants, under salt stress. Thus, P. minioluteum ameliorated the adverse effects of abiotic salinity stress and rescued soybean plant growth by influencing biosynthesis of the plant's hormones and flavonoids.

Keywords

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