Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Monitoring of Leuconostoc Population During Sauerkraut Fermentation by Quantitative Real-Time Polymerase Chain Reaction

  • Kim, So-Young (Department of Agrofood Resources, National Academy of Agricultural Science (NAAS), Rural Development Administration (RDA)) ;
  • Yoo, Ki-Seon (Department of Food Science and Technology, Research Center for Bioresource and Health, Chungbuk National University) ;
  • Kim, Yu-Jin (Department of Food Science and Technology, Research Center for Bioresource and Health, Chungbuk National University) ;
  • Seo, Eun-Young (Department of Food Science and Technology, Research Center for Bioresource and Health, Chungbuk National University) ;
  • Kim, Beom-Soo (Department of Chemical Engineering, Chungbuk National University) ;
  • Han, Nam-Soo (Department of Food Science and Technology, Research Center for Bioresource and Health, Chungbuk National University)
  • Received : 2011.02.14
  • Accepted : 2011.06.13
  • Published : 2011.10.28
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Abstract

A real-time PCR assay method was established to monitor Leuconostoc spp. populations via specific amplification of the dextransucrase gene. Quantification of L. mesenteroides B-512F using both genomic DNA and cell suspensions yielded a log-linear correlation spanning approximately 5 log units. By using this method, monitoring changes of Leuconostoc spp. during sauerkraut fermentation was successfully accomplished with accuracy after inoculation of starter and sugars (sucrose and maltose).

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References

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