Reproductive and Developmental Biology

The Korean Society of Animal Reproduction (한국동물번식학회)
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Effect of Magnetized Water on Cryopreservation in Bovine Spermatozoa

자기활성수가 소 정액 동결에 미치는 영향

  • Seo, Gi-Beom (College of Animal Life Sciences, Kangwon National University) ;
  • Lee, Yong-Seung (College of Animal Life Sciences, Kangwon National University) ;
  • Lee, Kyung-Jin (College of Animal Life Sciences, Kangwon National University) ;
  • Yu, Han-Jun (College of Animal Life Sciences, Kangwon National University) ;
  • Cheong, Hee-Tae (College of Veterinary Medicine, Kangwon National University) ;
  • Yang, Boo-Keun (College of Animal Life Sciences, Kangwon National University) ;
  • Lee, Seung-Hwan (National Institute of Animal Science, Hanwoo Ex) ;
  • Lee, Jin-Woo (ROHAS TECH) ;
  • Park, Choon-Keun (College of Animal Life Sciences, Kangwon National University)
  • 서기범 (강원대학교 동물생명과학대학) ;
  • 이용승 (강원대학교 동물생명과학대학) ;
  • 이경진 (강원대학교 동물생명과학대학) ;
  • 유한준 (강원대학교 동물생명과학대학) ;
  • 정희태 (강원대학교 수의학과) ;
  • 양부근 (강원대학교 동물생명과학대학) ;
  • 이승환 (국립축산과학원 한우시험장) ;
  • 이진우 ;
  • 박춘근 (강원대학교 동물생명과학대학)
  • Received : 2011.09.07
  • Accepted : 2011.09.19
  • Published : 2011.09.30
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Abstract

The purpose of this study was to improve of frozen-thawed sperm using magnetized water in Korean native cattle. Before cryopreservation, without egg-yolk Triladyl$^{(R)}$ solution was flowed though magnet [0, 2000, 4000 and 6000 Gauss(G)] for S min. The freezing of dilluted semen added with Triladyl containing 20% egg-yolk. Analysis of frozen-thawed sperm was estimated viability with SYBR14/PI double stain, membrane intact with hypoosmotic swelling test (HOST), acrosome reaction with FITC-PNA, mitochondria membrane function with Rhodamin 123 by flow- cytometry. Sperm viability was significantly higher in 4000G group than other groups (p<0.05). However, the Hypoosmotic Swelling Test(HOST) was significantly higher in fresh, 4000 and 6000G than 0 and 2000G (p<0.05). In addition, mitochondria membrane damage and acrosome damage were significantly lower in 6000G group than other groups (p<0.05). in conclusion we suggest that magnetized water could be improve ability of sperm on cryopreservation in Korean native cattle.

Keywords

References

  1. Gao DY, Mazur P, Critser JK (1997): Fundamental cryobiology of mammalian spermatozoa. In: Karow AM, Critser JK (eds) Reproductive Tissue Banking. Academic press, San Diego 263-327.
  2. Holt WV (2000): Basic aspects of frozen storage of semen. Anim Reprod Sci 62:3-22. https://doi.org/10.1016/S0378-4320(00)00152-4
  3. Huang SY, Kuo YH, Lee WC, Tsou HL, Lee YP, Chang HL, Yang JJ (1999): Substantial decrease of heatshock protein 90 precedes the decline of sperm motility during cooling of boar spermatozoa. Theriogenology 51:1007-1016. https://doi.org/10.1016/S0093-691X(99)00046-1
  4. Isachenko E (2003): Vitrification of mammalian spermatozoa in the absence of cryoprotectants: from past practical difficulties to present success. Reprod Biomed Online 6(2):191-200. https://doi.org/10.1016/S1472-6483(10)61710-5
  5. Kang BJ (2005): Magnetized Water. Seoul : Soe-Um Media Pub Co. : 49-63.
  6. Lednev VV (1991): Possible mechanism for the influence of weak magetic field on biological system. Bioelectromagnetics 12(2):71-75. https://doi.org/10.1002/bem.2250120202
  7. Ma YL, Ren H, Ren S, Zhen EK, Hao G, Zhao YW (1992): A study of the effect of magnetized water on enzyme activities by poten-tiometric enzyme electrode method. J Tongji Med Univ 12 (4):193-196. https://doi.org/10.1007/BF02887847
  8. Plummer JM, Watson PF (1985): Ultrastructural localization of calcium ions in ram spermatozoa before and after cold shock as demonstrated by a pyroantimonate technique. J Rprod Fertil 75:255-263. https://doi.org/10.1530/jrf.0.0750255
  9. Polge C, Smith AU, Parkes AS (1949): Revival of spermatozoa after vitrification and dehydration at low temperature. Nature 164:666-676. https://doi.org/10.1038/164666a0
  10. Purdy PH (2006): A review on goat sperm cryopreservation. Small Rum Res 6:215-225.
  11. Trina V (1980): Artificial insemination and semen banks in Italy. In: Human Artificial Insemination and Semen Preservation. Plenum, New York, p 51.
  12. Woo MW, Mujumdar AS (2010): Effects of electric and magnetic field on freezing and possible relevance in freeze drying. Drying Technology 28(4):433-443. https://doi.org/10.1080/07373930903202077
  13. Xu YB, Sun SY (2008): Effect of stable weak magnetic field on Cr (VI) bio-removal in anaerobic SBR system. Biodegradation 19(3):455-462. https://doi.org/10.1007/s10532-007-9151-5
  14. Zhang YS, Wu HW (1984): Effect of magnetic water on wrinary calculi-an experimental and clinical study. Acta Acad Med Wuhan 4(1):31-37. https://doi.org/10.1007/BF02856947
  15. Zhang YS, Wu HW (1987): Effect of magnetic water on wrinary calculi-an experimental and clinical study. Z Urol Nephrol 80(9):517-523.