폴리머 (Polymer(Korea))

  • 제35권5호
  • /
  • Pages.378-384
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  • 2011
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  • 0379-153X(pISSN)
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  • 2234-8077(eISSN)
한국고분자학회 (The Polymer Society of Korea)
권호 표지

실크 피브로인/젤라틴 하이브리드 지지체의 제조 및 특성분석

Preparation and Characterization of Silk Fibroin/Gelatin Hybrid Scaffolds

  • 김혜린 (전북대학교 BIN 융합공학과, 고분자 나노공학과) ;
  • 홍민성 (전북대학교 BIN 융합공학과, 고분자 나노공학과) ;
  • 김수진 (전북대학교 BIN 융합공학과, 고분자 나노공학과) ;
  • 조한수 (전북대학교 BIN 융합공학과, 고분자 나노공학과) ;
  • 유일수 (전북대학교 BIN 융합공학과, 고분자 나노공학과) ;
  • 이동원 (전북대학교 BIN 융합공학과, 고분자 나노공학과) ;
  • 강길선 (전북대학교 BIN 융합공학과, 고분자 나노공학과)
  • Kim, Hye-Lin (Department of BIN Fusion Technology, Department of Polymer.Nano Science Technology, Polymer Fusion Research Center, Chonbuk National University) ;
  • Hong, Min-Sung (Department of BIN Fusion Technology, Department of Polymer.Nano Science Technology, Polymer Fusion Research Center, Chonbuk National University) ;
  • Kim, Su-Jin (Department of BIN Fusion Technology, Department of Polymer.Nano Science Technology, Polymer Fusion Research Center, Chonbuk National University) ;
  • Jo, Han-Su (Department of BIN Fusion Technology, Department of Polymer.Nano Science Technology, Polymer Fusion Research Center, Chonbuk National University) ;
  • Yoo, Il-Sou (Department of BIN Fusion Technology, Department of Polymer.Nano Science Technology, Polymer Fusion Research Center, Chonbuk National University) ;
  • Lee, Dong-Won (Department of BIN Fusion Technology, Department of Polymer.Nano Science Technology, Polymer Fusion Research Center, Chonbuk National University) ;
  • Khang, Gil-Son (Department of BIN Fusion Technology, Department of Polymer.Nano Science Technology, Polymer Fusion Research Center, Chonbuk National University)
  • 투고 : 2010.09.30
  • 심사 : 2011.04.12
  • 발행 : 2011.09.25
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초록

실크 피브로인은 생체적합성과 비독성 및 비면역 특성을 갖는 생분해성 천연고분자로서, 콜라겐의 가수분해로부터 유래되는 천연물질인 젤라틴을 이용하여 실크 피브로인/젤라틴 지지체를 제조하였다. 지지체의 최적화 조건을 찾기 위하여 실크 피브로인의 양과 젤라틴 및 글루타알데히드의 농도를 다르게 하여 제조하였다. 실크 피브로인/젤라틴 지지체는 SEM과 DSC 및 수분흡수성 평가를 통해 특성분석을 하였으며 세포생존율 및 증식률은 WST 방법을 통해 평가되었다. 이 결과 실크 피브로인 0.3 g 지지체에 8% 젤라틴 및 1% 글루타알데히드를 함유한 지지체에서 세포 부착 및 증식을 위해 가장 적합한 특성을 제공한다고 제안되었다. 결과적으로, 실크 피브로인/젤라틴 지지체는 잠재적인 세포 전달체 및 조직공학을 위한 구조 기반역할을 할 수 있을 것으로 사료된다.

Silk fibroin is a biocompatible and slowly biodegradable natural polymer. This natural polymer has excellent mechanical properties, non-toxicity, and non-immunogenic properties and has been demonstrated to support tissue regeneration. Also, gelatin is a natural material derived from collagen by hydrolysis and has an almost identical composition as that of collagen. Silk fibroin/gelatin scaffolds have been fabricated by using the freeze-drying method. To establish the scaffold manufacturing condition for silk fibroin and gelatin, we made scaffolds with various compositions of gelatin, glutaldehyde and silk fibroin. The silk fibroin/gelatin scaffolds were characterized using SEM, DSC, and water absorption ability tests. The cellular proliferation was evaluated by WST assay. These results suggested that a scaffold containing 8% of gelatin, 1% of glutaldehyde and 0.3 g of silk fibroin provided suitable characterstics for cell adhesion and proliferation. In conclusion, the silk fibroin/gelatin scaffold may serve as a potential cell delivery vehicle and a structural basis for tissue engineering.

키워드

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