한국육종학회지 (Korean Journal of Breeding Science)

  • 제42권5호
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  • Pages.481-487
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  • 2010
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  • 0250-3360(pISSN)
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  • 2287-5174(eISSN)
한국육종학회 (The Korean Society of Breeding Science)
권호 표지

Changes in Polygalacturonase and Ethylene Biosynthesis of Three Varieties of Apple During Fruit Ripening

  • Kim, Se Hee (Fruit Research Division, National Institute of Horticultural & Herbal Science, RDA) ;
  • Han, Sang Eun (Research Service Division, SolGent. Co., Ltd) ;
  • Lee, Hye Eun (Vegetable Research Division, National Institute of Horticultural & Herbal Science, RDA) ;
  • Cho, Mi-Ae (Fruit Research Division, National Institute of Horticultural & Herbal Science, RDA) ;
  • Shin, Il Sheob (Fruit Research Division, National Institute of Horticultural & Herbal Science, RDA) ;
  • Kim, Jeong-Hee (Fruit Research Division, National Institute of Horticultural & Herbal Science, RDA) ;
  • Cho, Kang-Hee (Fruit Research Division, National Institute of Horticultural & Herbal Science, RDA) ;
  • Kim, Dae-Hyun (Fruit Research Division, National Institute of Horticultural & Herbal Science, RDA) ;
  • Hwang, Jeong Hwan (Fruit Research Division, National Institute of Horticultural & Herbal Science, RDA)
  • 투고 : 2010.12.16
  • 발행 : 2010.12.31
⟨ 이전 논문 다음 논문 ⟩

초록

The ripening behavior of three apple cultivars, 'Tsugaru', 'Hongro' and 'Fuji' was distinctive and the involvement of POLYGALACTURONASE(PG) in the fruit softening process was confirmed to be ethylene dependent. Fruit softening is genetically coordinated by the action of several cell wall enzymes, including PG which depolymerizes cell wall pectin. Also, loss of firmness is associated with increasing of the ripening hormone, ethylene. In this work, climacteric ripening of three apple cultivars, Tsugaru, Hongro and Fuji, producing different ethylene levels and ripening responses, was examined. Correspondingly in Fuji, a linear and basal ethylene level was observed over the entire period of measurements, and Tsugaru and Hongro displayed a typical climacteric rise in ethylene production. Transcript accumulation of genes involved in ethylene biosynthesis (MdACS3 and MdACO1) and MdPG1 was studied in Tsugaru, Hongro and Fuji cultivars. Expression of MdACO1 transcripts was shown in all three ripened apple fruits. However, the MdACS3 and MdPG1 were transcribed differently in these cultivars. Comparing the MdPG1 of 'Tsugaru', 'Hongro' and 'Fuji', structural difference was discovered by genomic Southern analysis. Overall results pointed out that MdACS3 and MdPG1 play an important role in regulation of fruit ripening in apple cultivar.

키워드

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