FLOWER RESEARCH JOURNAL (화훼연구)

The Korean Society for Floricultural Science (한국화훼학회)
권호 표지

Dormancy Breaking by Hot-water Treatment of Lilium Oriental Hybrids Bulblets Regenerated in Vitro

온탕처리에 의한 오리엔탈나리 소인경의 휴면 타파

  • Woo, Jin Ha (Gumi Floricultural Experiment Station, Gyeongbuk Agricultural Research and Extension Services) ;
  • Nam, Hyo Hoon (Gyeongbuk Agricultural Research and Extension Services) ;
  • Lee, Hyun Suk (Gumi Floricultural Experiment Station, Gyeongbuk Agricultural Research and Extension Services) ;
  • Choi, Dong Jin (Gumi Floricultural Experiment Station, Gyeongbuk Agricultural Research and Extension Services) ;
  • Oh, Wook (Department of Horticultural Science, Yeunganm University) ;
  • Kim, Kiu Weon (Department of Horticultural Science, Yeunganm University)
  • 우진하 (경북농업기술원 구미화훼시험장) ;
  • 남효훈 (경북농업기술원) ;
  • 이현숙 (경북농업기술원 구미화훼시험장) ;
  • 최동진 (경북농업기술원 구미화훼시험장) ;
  • 오욱 (영남대학교 원예학과) ;
  • 김규원 (영남대학교 원예학과)
  • Received : 2010.04.08
  • Accepted : 2010.04.26
  • Published : 2010.06.30
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Abstract

The objective of this study was to investigate the effect of hot water and dipping times, and hot- PGR's solution on dormancy breaking of Lilium Oriental Hybrid 'Casa Blanca' and 'Siberia' bulblets regenerated in vitro. Hot-water treatments with distilled water were effective not sprouting rate but shortening of days to sprouting. While $100mg{\cdot}L^{-1}\;GA_{4+7}$ was effective in the dormancy breaking process of bulblets. The content of mitochondrial protein and fumarase activity were highest in bulblet of $100mg{\cdot}L^{-1}\;GA_{4+7}$ addition in hot water treatment. Therefore $GA_{4+7}$ addition in hot-water treatment seem to be the alternative dormancy breaking method to low-temperature treatment in Lilium Oriental Hybrid bulblet regenerated in vitro.

본 연구는 기내에서 재생된 오리엔탈나리 'Casa Blanca'와 'Siberia' 소인경의 새로운 휴면 타파방법을 모색하고자 생장조절물질을 첨가한 온탕처리 조건을 검토하였다. 오리엔탈나리 'Casa Blanca'와 'Siberia' 두 품종 모두 $30^{\circ}C$ 증류수, 120분 온탕처리에서 맹아소요일수는 단축되었으나 맹아율이 낮았다. $GA_{4+7}$ $100mg{\cdot}L^{-1}$용액을$35^{\circ}C$ 에서 1시간 온탕 처리에 의해서 'Casa Blanca'와 'Siberia'의 맹아율은 각각 76.7%으로 크게 촉진되었고 정식 2년차의 생장과 구근 비대가 촉진되었으며, 휴면에 따른 호흡정도를 반영하는 미토콘드리아 단백질 함량과 fumarase 활성도 높아 휴면타파의 생리적 원인도 구명하였다. 생장조절물질($GA_{4+7}$)을 첨가한 온탕처리에 의해 기내에서 재생된 오리엔탈나리 소인경의 휴면을 효과적으로 타파할 수 있었다.

Keywords

References

  1. Choi, S.T. 1983. Effects of low temperature and hot water treatment to the dormant bulbs on the leaf emergence of bulblets during scale propagation of Ester lily (Lilium longiflorum Thunb.). J. Kor. Soc. Hort. Sci. 24:42-48.
  2. Juntilla, O. 1988. To be or not to be dormant: some comments on the new dormancy nomenclature. HortScience 23:805-860.
  3. Kamerbeek, G.A., T.C.M. Beijersbergen, and P.K. Schenk. 1972. Dormancy in bulbs and corms. Proc. 18th Int. Hort. Congr. 5:233-239.
  4. Kim, K.S. 1991. The effect of growth regulators, temperature and sucrose on the dormancy in Lilium speciosum bulblets cultured in vitro. Kor. J. Pant Tissue Culture. 18:103-111.
  5. Kim, K.W. and A.A. De Hertogh. 1997. Tissue culture of ornamental flowering bulbs (geophytes). Hort Rev. 18:87-169.
  6. Kim, K.W., J.S. Kim, E.Y. Kim, J.D. Choi, and K.I. Park. 1998. Effects of culture condition on dormant status of in vitro regenerated Lilium Oriental Hybrids bulblets. J. Kor. Soc. Hort. Sci. 39:641-646.
  7. Kim, K.W., K.A. Kim, and M.S. Byun. 1999. Physiological changes before and after breaking dormancy of Lilium hansonii regenerated in vitro. J. Kor. Soc. Hort. Sci. 40:751-754.
  8. Kim, K.W., S.Y. Moon, and S.J. Eum. 2003. Shortening dormancy breaking period by $GA_{4+7}$ addition in hot-water treatment of Korea native Lilium bulblets regenerated in vitro. J. Kor. Soc. Hort. Sci. 44:744-747.
  9. Lowry, O.H., N.J. Rosebrough, A.L. Farr, and R.j. Randall. 1951. Protein measurement with the folin phenol reagent. J. Biol. Chem. 193-275.
  10. Niimi, Y., Y. Endo, and E. Arisaka. 1988. Effects of chilling and $GA_{3}$ treatments on breaking dormancy in Lilium rubellum Baker bulblets culture in vitro. J. Jpn. Soc. Hort. Sci. 57:250-257. https://doi.org/10.2503/jjshs.57.250
  11. Moon, S.Y. 2002. Dormancy breaking by chilling and hot-water treatment of Lilium davuricum and L. hansonii bulblets regenerated in vitro. MS Thesis. Yeungnam University. Geyeongsan, Korea.
  12. Racker, E. 1950. Spectrophotometric measurements of the enzymatic formation of fumaric acid cis-aconitic acid. Bioochem. Biophys. Acta. 4:211-214. https://doi.org/10.1016/0006-3002(50)90026-6
  13. Roh, S.M. and H.F. Wilkins. 1976. The physiology of dormancy and maturity of Lilium longiflorum Thunb. cv. Nellie White bulb. J. Kor. Soc. Hort. Sci. 17:173-179.
  14. Stimart, D.P. and D.P. Ascher. 1981. Developmental responses of Lilium longiflorum bulblets to constant or alternating temperature in vitro. J. Amer. Soc. Hort. Sci. 106:450-454.
  15. Stimart, D.P., D.P. Ascher, and H.F. Wilkins. 1982. Overcoming dormancy in Lilium longiflorum bulblets produced in tissue culture. J. Amer. Soc. Hort. Sci. 107:1004-1007.
  16. Woo, J.H., H.H. Nam, K.B. Choi, I.S. Park, and K.W. Kim. 2003. Dormancy and culture temperature of Lilium Oriental Hybrid bulblets regenerated in vitro. J. Kor. Flower Res. Soc. 11:143-147.
  17. Woo, J.H., Y.G. Sim, Y.Y. Han, H.H. Nam, K.B. Choi, and K.W. Kim. 2001. Effect of culture medium and it's physico-chemical properties on bulblet growth of Lilium Oriental Hybrid. J. Kor. Soc. Hort. Sci. 42:465-468.