한국산림과학회지 (Journal of Korean Society of Forest Science)

  • 제99권2호
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  • Pages.172-178
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  • 2010
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  • 2586-6613(pISSN)
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  • 2586-6621(eISSN)
한국산림과학회 (Korean Society of Forest Science)
권호 표지

Investigation of Ectomycorrhizal Fungal Colonization in Pinus thunbergii Seedlings at a Plantation Area in Gangneung, using Morphotyping and Sequencing the rDNA Internal Transcribed Spacer Region

  • Obase, Keisuke (College of Forest and Environmental Sciences, Kangwon National University) ;
  • Cha, Joo-Young (Field Science Center for Northern Biosphere, Hokkaido University) ;
  • Lee, Jong-Kyu (College of Forest and Environmental Sciences, Kangwon National University) ;
  • Lee, Sang-Yong (College of Forest and Environmental Sciences, Kangwon National University) ;
  • Lee, Jin-Ho (College of Forest and Environmental Sciences, Kangwon National University) ;
  • Chun, Kun-Woo (College of Forest and Environmental Sciences, Kangwon National University)
  • 투고 : 2009.11.18
  • 심사 : 2010.02.11
  • 발행 : 2010.04.30
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초록

The status of ectomycorrhizal (ECM) fungal colonization in Pinus thunbergii seedlings was investigated 2 years after planting in an eastern coastal area of Korea. We established three $10{\times}10$ m plots at a P. thunbergii plantation in Gangneung and sampled lateral roots from 10 seedlings in each plot. ECMs were classified into morphological groups and the number of root tips of each morphotype was counted. In total, 8 ECM morphotypes were observed and fungal species that form each morphotype were identified by sequencing of the internal transcribed spacer (ITS) region of the nuclear rDNA. Suillus granulatus was the most abundant species (44.1-65.7% of relative abundance) in all plots, followed by Tomentella ellisii (14.0-37.8%) and unidentified fungus belonged to Atheliaceae (10.6-20.1%). These 3 fungal species accounted for almost all of the ECM abundance in each plot (94.9-99.8%). The remaining 5 fungal species were uncommon and rare. There was no clear difference in ECM fungal communities among plots. Community structure of ECM fungi in the young P. thunbergii plantation was simple and composed of fungal species that were also observed in mature coastal pine forests.

키워드

참고문헌

  1. Agerer, R. 1995. Anatomical characteristics of identified ectomycorrhizas: an attempt towards a natural classification. In: Varma, A.K. and Hock, B. (Eds.), Mycorrhiza: Structure, function, molecular biology and biotechnology. Springer, Berlin Heidelberg New York, 685-734.
  2. Baxter, J.W. and Dighton, J. 2001. Ectomycorrhizal diversity alters growth and nutrient acquisition of grey birch (Betula populifolia) seedlings in host-symbiont culture conditions. New Phytologist 152:139-149. https://doi.org/10.1046/j.0028-646x.2001.00245.x
  3. Choi, M.G. 1986. Characteristics of salt tolerance in tree species. Journal of Korean Forestry Society 29(2): 1-8. (In Korean with English abstract)
  4. Chun, K.W., Lee, J.H., Kim, K.N., Yeom, K.J., Seo, J.C., Ma, H.S., Park, M.S. and Ezaki, T. 2008. The present status and future task of seaside protection forest in Korea. Journal of the Japanese Society of Coastal Forest 7(2): 21-25. (In Japanese with English abstract)
  5. Colwell, R.K. 2005. EstimateS: Statistical estimation of species richness and shared species from samples. Version 7.5. [http://purl.oclc.org/estimates].
  6. Cram, M.M., Mexal, J.G. and Souter, R. 1999. Successful reforestation of south Carolina sandhills is not influenced by seedling inoculation with Pisolithus tinctorius in the nursery. Southern Journal of Applied Forestry 23: 46-52.
  7. Cullings, K.W., New, M.H., Makhija, S. and Parker, V.T. 2003. Effects of litter addition on ectomycorrhizal associates of a lodgepole pine (Pinus contorta) stand in Yellowstone National Park. Applied and Environmental Microbiology 69: 3772-3776. https://doi.org/10.1128/AEM.69.7.3772-3776.2003
  8. Dunstan, W.A., Dell, B. and Malajczuk, N. 1998. The diversity of ectomycorrhizal fungi associated with introduced Pinus spp. in the Southern Hemisphere, with particular reference to Western Australia. Mycorrhiza 8: 71-79. https://doi.org/10.1007/s005720050215
  9. Gardes, M. and Bruns, T.D. 1993. ITS primers with enhanced specificity for basidiomycetes: application to the identification of mycorrhizae and rusts. Molecular Ecology 2: 113-118. https://doi.org/10.1111/j.1365-294X.1993.tb00005.x
  10. Horton, T.R. and Bruns, T.D. 1998. Multiple host fungi are the most frequent and abundant ectomycorrhizal types in a mixed stand of Douglas fir (Pseudotsuga menziesii (Mirb.) Franco) and bishop pine (Pinus muricata D. Don). New Phytologist 139: 331-339. https://doi.org/10.1046/j.1469-8137.1998.00185.x
  11. Ingleby, K., Mason, P.A., Last, F.T. and Fleming, L.V. 1990. Identification of ectomycorrhizas. ITE Research Publication No. 5, HMSO, London.
  12. Jacobson, K.M., Miller, O.K.Jr. and Turner, B.J. 1993. Randomly amplified polymorphic DNA markers are superior to somatic incompatibility tests for discriminating genotypes in natural populations of the ectomycorrhizal fungus Suillus granulatus. Proceeding of the National Academy of Sciences of the United States of America 90: 9159-9163. https://doi.org/10.1073/pnas.90.19.9159
  13. Jonsson, L., Dahlberg, A., Nilsson, M.C., Kårén, O. and Zackrisson, O. 1999. Continuity of ectomycorrhizal fungi in self-regenerating boreal Pinus sylvestris forests studied by comparing mycobiont diversity on seedlings and mature trees. New Phytologist 142: 151-162. https://doi.org/10.1046/j.1469-8137.1999.00383.x
  14. Kataoka, R., Taniguchi, T., Ooshima, H. and Futai, K., 2008. Comparison of the bacterial communities established on the mycorrhizae formed on Pinus thunbergii root tips by eight species of fungi. Plant and Soil 304: 267-275. https://doi.org/10.1007/s11104-008-9548-x
  15. Konta, F. 2001. The present conditions and functions of the coastal forests in Japan. Journal of the Japanese Society of Coastal Forest 1(1): 1-4. (In Japanese with English abstract)
  16. Kretzer, A., Li, Y., Szaro, T. and Bruns, T.D. 1996. Internal transcribed spacer sequences from 38 recognized species of Suillus sensu lato: Phylogenetic and taxonomic implications. Mycologia 88: 776-785. https://doi.org/10.2307/3760972
  17. Lee, C.Y., Jeong, Y.H. and Kim, J.H. 2005. Actual condition and management plan for the coastal prevention forest. International Symposium proceeding of the Japanese Society of Coastal Forest and the Korea Society of Coastal Forest: 54-55. (In Korean)
  18. Manian, S., Sreenivasaprasad, S., Bending, G.D. and Mills, P.R. 2001. Genetic diversity and interrelationships among common European Suillus species based on ribosomal DNA sequences. FEMS Microbiology Letters 204: 117-121. https://doi.org/10.1111/j.1574-6968.2001.tb10873.x
  19. Matsuda, Y. and Hijii, N. 2004. Ectomycorrhizal fungal communities in an Abies firma forest, with special reference to ectomycorrhizal associations between seedlings and mature trees. Canadian journal of Botany 82: 822-829. https://doi.org/10.1139/b04-065
  20. Matsuda, Y., Hayakawa, N. and Ito, S. 2009. Local and microscale distributions of Cenococcum geophilum in soils of coastal pine forests. Fungal Ecology 2: 31-35. https://doi.org/10.1016/j.funeco.2008.10.002
  21. Nara, K. and Hogetsu, T. 1996. Effects of inoculation with ectomycorrhizal fungus to seven Pinus species including several foreign pines. Transactions of the Japanese Forestry Society 107: 227-228. (In Japanese)
  22. Obase, K., Tamai, Y., Yajima, T. and Miyamoto, T. 2007. Mycorrhizal associations in woody plant species at the Mt. Usu volcano, Japan. Mycorrhiza 17: 209- 215. https://doi.org/10.1007/s00572-006-0097-y
  23. Obase, K., Cha, J.Y., Lee, J.K., Lee, S.Y., Lee, J.H. and Chun, K.W. 2009a. Ectomycorrhizal fungal communities associated with Pinus thunbergii in the eastern coastal pine forests of Korea. Mycorrhiza 20: 39-49. https://doi.org/10.1007/s00572-009-0262-1
  24. Obase, K., Tamai, Y., Yajima, T. and Miyamoto, T. 2009b. Mycorrhizal synthesis of four ectomycorrhizal fungi in potted Populus maximowiczii seedlings. Mycoscience 50: 143-145. https://doi.org/10.1007/s10267-008-0457-x
  25. Park, W.G., Yi, M.J., Chun, K.W. and Ezaki, T. 2002. A study on stand structure of Pinus thunbergii Parl. Forests in eastern part of Kangwon-do, Korea. Journal of the Japanese Society of Coastal Forest 1(2): 13-18. (In Japanese with English abstract)
  26. Pera, J. and Alvarez, I.F. 1995. Ectomycorrhizal fungi of Pinus pinaster. Mycorrhiza 5: 193-200. https://doi.org/10.1007/BF00203337
  27. Sims, K., Watling, R., De la Cruz, R. and Jeffries, P. 1997. Ectomycorrhizal fungi of the Philippines: a preliminary survey and notes on the geographic biodiversity of the Sclerodermatales. Biodiversity and Conservation 6: 45-58.
  28. Smith, S.E. and Read, D.J. 1997. Mycorrhizal symbiosis, 2nd edn. London, UK, Academic Press.
  29. Taniguchi, T., Kanzaki, N., Tamai, S., Yamanaka, N. and Futai, K. 2007. Does ectomycorrhizal fungal community structure vary along a Japanese black pine (Pinus thunbergii) to black locust (Robinia pseudoacacia) gradient? New Phytologist 173: 322-334. https://doi.org/10.1111/j.1469-8137.2006.01910.x
  30. Taylor, D.L. and Bruns, T.D. 1999. Community structure of ectomycorrhizal fungi in a Pinus muricata forest: minimal overlap between the mature forest and resistant propagule communities. Molecular Ecology 8: 1837-1850. https://doi.org/10.1046/j.1365-294x.1999.00773.x
  31. Valentine, L.L., Fiedler, T.L., Hart, A.N., Petersen, C.A., Berninghausen, H.K. and Southworth, D. 2004. Diversity of ectomycorrhizas associated with Quercus garryana in southern Oregon. Canadian Journal of Botany 82: 123-135. https://doi.org/10.1139/b03-117
  32. Wiemken, V. and Boller, T. 2006. Delayed succession from alpine grassland to savannah with upright pine: Limitation by ectomycorrhiza formation? Forest Ecology and Management 237: 492-502. https://doi.org/10.1016/j.foreco.2006.09.084
  33. Yamada, A. and Katsuya, K. 1995. Mycorrhizal association of isolates from sporocarps and ectomycorrhizas with Pinus densiflora seedlings. Mycoscience 36:315- 323. https://doi.org/10.1007/BF02268607