Anatomy and Cell Biology

Korean Association of Anatomists (대한해부학회)
권호 표지
DOI QR코드

DOI QR Code

Chromatin organization and transcriptional activation of Hox genes

  • Received : 2010.02.03
  • Accepted : 2010.03.05
  • Published : 2010.03.30
⟨ Previous Next ⟩

Abstract

Spatially and temporally programmed expression of the Hox genes along the antero-posterior (A-P) axis is essential for correct pattern formation during embryonic development. An accumulating body of evidence indicates the pivotal role of spatial chromatin organization for the coordination of gene regulation. Recently, chromosome conformation capture (3C) technique has been developed and opened a new way to study chromosomal interactions in the nucleus. In this study, we describe 3C method we applied in F9 embryonic teratocarcinoma cells and demonstrate that the chromosomal interactions at Hox loci are successfully detected. Interestingly, at Hoxc loci, the abundance of intrachromosomal interactions with neighboring fragments was drastically decreased when the genes are expressed. These results indicate the possibility of the dynamic pattern of chromosomal interaction in association with the transcriptional regulation of Hox genes.

Keywords

Acknowledgement

Grant : BioGreen21 Program

Supported by : NRF, RDA

References

  1. Boncinelli E, Simeone A, Acampora D, Mavilio F. (1991). HOX gene activation by retinoic acid. Trends Genet 7: 329-334 https://doi.org/10.1016/0168-9525(91)90423-N
  2. Boney-Montoya J, Ziegler YS, Curtis CD, Montoya JA, Nardulli AM. (2010). Long-Range transcriptional control of progesterone receptor gene expression. Mol Endocrinol 24: 346-358 https://doi.org/10.1210/me.2009-0429
  3. Breier G, Bućan M, Francke U, Colberg-Poley AM, Gruss P. (1986). Sequential expression of murine homeo box genes during F9 EC cell diff erentiation. EMBO J 5: 2209-2215
  4. Carter D, Chakalova L, Osborne CS, Dai YF, Fraser P. (2002). Long-range chromatin regulatory interactions in vivo. Nat Genet 32: 623-626 https://doi.org/10.1038/ng1051
  5. Chambeyron S, Bickmore WA. (2004). Chromatin decondensation and nuclear reorganization of the HoxB locus upon induction of transcription. Genes Dev 18: 1119-1130 https://doi.org/10.1101/gad.292104
  6. Chavanas S, Adoue V, Mechin MC, et al. (2008). Long-range enhancer associated with chromatin looping allows AP-1 regulation of the peptidylarginine deiminase 3 gene in diff erentiated keratinocyte. PLoS ONE 3: e3408 https://doi.org/10.1371/journal.pone.0003408
  7. Dekker J. (2008). Ggene regulation in the third dimension. Science 319: 1793-1794 https://doi.org/10.1126/science.1152850
  8. Dekker J, Rippe K, Dekker M, Kleckner N. (2002). Capturing chromosome conformation. Science 295: 1306-1311 https://doi.org/10.1126/science.1067799
  9. Fraser J, Rousseau M, Shenker S, et al. (2009). Chromatin conformation signatures of cellular differentiation. Genome Biology 10: R37 https://doi.org/10.1186/gb-2009-10-4-r37
  10. Gaunt SJ, Strachan L. (1996). Temporal colinearity in expression of anterior hox genes in developing chick embryos. Dev Dyn 207: 270-280 https://doi.org/10.1002/(SICI)1097-0177(199611)207:3<270::AID-AJA4>3.0.CO;2-E
  11. Izpisúa-Belmonte JC, Falkenstein H, Dollé P, Renucci A, Duboule D. (1991). Murine genes related to the Drosophila AbdB homeotic genes are sequentially expressed during development of the posterior part of the body. EMBO J 10: 2279-2289
  12. Kleinjan DA, Lettice LA, Veronica van H, Robert EH. (2008). Chapter 13 long range gene control and genetic disease. Advances in genetics, academic press. Volume 61: 339-388.
  13. Lomvardas S, Barnea G, Pisapia DJ, Mendelsohn M, Kirkland J, Axel R. (2006). Interchromosomal interactions and olfactory receptor choice. Cell 126: 403-413 https://doi.org/10.1016/j.cell.2006.06.035
  14. Murphy SP, Garbern J, Odenwald WF, Lazzarini RA, Linney E. (1988). Differential expression of the homeobox gene Hox-1.3 in F9 embryonal carcinoma cells. Proc Natl Acad Sci USA 85: 5587-5591 https://doi.org/10.1073/pnas.85.15.5587
  15. Murrell A, Heeson S, Reik W. (2004). Interaction between diff erentially methylated regions partitions the imprinted genes Igf2 and H19 into parent-specific chromatin loops. Nat Genet 36: 889-893 https://doi.org/10.1038/ng1402
  16. Spilianakis CG, Flavell RA. (2004). Long-range intrachromosomal interactions in the T helper type 2 cytokine locus. Nat Immunol 5: 1017-1027 https://doi.org/10.1038/ni1115
  17. Stornaiuolo A, Acampora D, Pannese M, et al. (1990). Human HOX genes are diff erentially activated by retinoic acid in embryonal carcinoma cells according to their position within the four loci. Cell Diff er Dev 31: 119-127 https://doi.org/10.1016/0922-3371(90)90015-O
  18. Tolhuis B, Palstra R-J, Splinter E, Grosveld F, de Laat W. (2002). Looping and interaction between hypersensitive sites in the active beta-globin locus. Mol Cell 10: 1453-1465 https://doi.org/10.1016/S1097-2765(02)00781-5
  19. Wang KC, Helms JA, Chang HY. (2009). Regeneration, repair and remembering identity: the three Rs of Hox gene expression. Trends Cell Biol 19: 268-275 https://doi.org/10.1016/j.tcb.2009.03.007
  20. Wurtele H, Chartrand P. (2006). Genome-wide scanning of HoxB1-associated loci in mouse ES cells using an openended chromosome conformation capture methodology. Chromosome Res 14: 477-495 https://doi.org/10.1007/s10577-006-1075-0
  21. Yu SJ, Lee JY, Kim SH, Deocaris CC, Kim MH. (2009). Synthetic maternal stress hormone can modulate the expression of Hox genes. J Exp Biomed Sci 15: 249-255

Cited by

  1. F9 EC 세포에서 레티노산에 의해 유도되는 Hoxc 유전자의 발현에 히스톤 메틸화가 미치는 영향 vol.25, pp.6, 2015, https://doi.org/10.5352/jls.2015.25.6.703
  2. A long-range interactive DNA methylation marker panel for the promoters of HOXA9 and HOXA10 predicts survival in breast cancer patients vol.9, pp.None, 2010, https://doi.org/10.1186/s13148-017-0373-z
  3. Wnt/β-catenin Pathway-Mediated PPARδ Expression during Embryonic Development Differentiation and Disease vol.22, pp.4, 2010, https://doi.org/10.3390/ijms22041854