Anatomy and Cell Biology

Korean Association of Anatomists (대한해부학회)
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Immunohistochemical study on the expre ssion of calcium binding proteins (calbindin-D28k, calretinin, and parvalbumin) in the cerebellum of the nNOS knock-out(-/-) mice

  • Lee, Jae-Chul (Department of Anatomy, Seoul National University College of Medicine) ;
  • Chung, Yoon-Hee (Chung-Ang University College of Medicine) ;
  • Cho, Yu-Jin (Department of Anatomy, Seoul National University College of Medicine) ;
  • Kim, Jan-Di (Department of Anatomy, Seoul National University College of Medicine) ;
  • Kim, Na-Hee (Department of Anatomy, Seoul National University College of Medicine) ;
  • Cha, Choong-Ik (Department of Anatomy, Seoul National University College of Medicine) ;
  • Joo, Kyeung-Min (Department of Anatomy, Seoul National University College of Medicine)
  • Received : 2010.02.04
  • Accepted : 2010.03.02
  • Published : 2010.03.30
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Abstract

Nitric Oxide (NO) actively participates in the regulation of neuronal intracellular $Ca^{2+}$ levels by modulating the activity of various channels and receptors. To test the possibility that modulation of $Ca^{2+}$ buff er protein expression level by NO participates in this regulatory effect, we examined expression of calbindin-D28k, calretinin, and parvalbumin in the cerebellum of neuronal NO synthase knock-out ($nNOS^{(-/-)}$) mice using immunohistochemistry. We observed that in the cerebellar cortex of the $nNOS^{(-/-)}$ mice, expression of calbindin-D28k and parvalbumin were significantly increased while expression of calretinin was signifi cantly decreased. These results suggest another mechanism by which NO can participate in the regulation of $Ca^{2+}$ homeostasis.

Keywords

Acknowledgement

Supported by : BK21 project for Medicine

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