Journal of Applied Biological Chemistry

The Korean Society for Applied Biological Chemistry (한국응용생명화학회)
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Sterols from the Seed of Cowpea (Vigna sinensis K.)

동부로부터 sterol의 분리 동정

  • Cui, En-Ji (The Graduate School of Biotechnology & Plant Metabolism Research Center, Kyung Hee University) ;
  • Park, Hee-Jung (The Graduate School of Biotechnology & Plant Metabolism Research Center, Kyung Hee University) ;
  • Wu, Qian (The Graduate School of Biotechnology & Plant Metabolism Research Center, Kyung Hee University) ;
  • Chung, In-Sik (The Graduate School of Biotechnology & Plant Metabolism Research Center, Kyung Hee University) ;
  • Kim, Ji-Young (The Graduate School of Biotechnology & Plant Metabolism Research Center, Kyung Hee University) ;
  • Baek, Nam-In (The Graduate School of Biotechnology & Plant Metabolism Research Center, Kyung Hee University)
  • 최은희 (경희대학교 생명공학원 및 식물대사연구센터) ;
  • 박희정 (경희대학교 생명공학원 및 식물대사연구센터) ;
  • 오청 (경희대학교 생명공학원 및 식물대사연구센터) ;
  • 정인식 (경희대학교 생명공학원 및 식물대사연구센터) ;
  • 김지영 (경희대학교 생명공학원 및 식물대사연구센터) ;
  • 백남인 (경희대학교 생명공학원 및 식물대사연구센터)
  • Received : 2009.11.04
  • Accepted : 2010.03.19
  • Published : 2010.06.30
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Abstract

The seed of cowpea (Vigna sinensis K.) was extracted with 80% aqueous methanol (MeOH). And the concentrated extract was partitioned with ethyl acetate (EtOAc), n-butanol (n-BuOH) and $H_2O$, successively. The repeated silica gel and octadecyl silica gel (ODS) column chromatographic separations for the EtOAc and n-BuOH fractions led to isolation of four sterols. And the chemical structures of the compounds were determined as a mixture of stigmasterol and $\beta$-sitosterol with the ratio of 4 to 3 (1), 7-ketositosterol (2), and stigmasterol 3-O-$\beta$D-glucopyranoside (3) from the interpretation of spectroscopic data including nuclear magnetic resonance (NMR) spectrum metric, mass (MS) spectrum metric and infrared (IR) spectroscope. This study reports the first isolation of $\beta$-sitosterol, 7-ketositosterol, and stigmasterol 3-O-$\beta$-D-glucopyranoside from the seed of Vigna sinensis K. In addition, compound 2, 7-ketositosterol, is rarely occurred in natural source including plant.

동부를 80% MeOH로 추출하고, 얻어진 추출물을 EtOAc, n-BuOH 및 $H_2O$로 용매 분획 하였다. 이 중 EtOAc과 n-BuOH 분획으로부터 silica gel과 octadecyl silica gel(ODS) column chromatography를 반복하여 4종의 sterol 화합물을 분리, 정제하였다. 각 화합물의 화학구조는 NMR, MS 및 IR 등의 spectrum data를 해석하여, stigmasterol(1a), $\beta$-sitosterol(1b), 7-ketositosterol(2) 및 stigmasterol 3-O-$\beta$-D-glucopyranoside(3)로 동정하였다. 화합물 1b, 2와 3은 동부에서 처음 분리되었다.

Keywords

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