International Journal of Oral Biology

The Korean Academy of Oral Biology (대한구강생물학회)
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Effects of Chitosan on the Differentiation of MDPC-23 Cells

  • Park, Ju-Hyun (Oral Biology Research Institute, Chosun University School of Dentistry) ;
  • Kim, Do-Kyung (Oral Biology Research Institute, Chosun University School of Dentistry) ;
  • Park, Jong-Tae (Division in Anatomy and Developmental Biology, Department of Oral Biology, Brain Korea 21 Project, Yonsei University College of Dentistry) ;
  • Kim, Su-Young (Oral Biology Research Institute, Chosun University School of Dentistry) ;
  • Yu, Sun-Kyoung (Oral Biology Research Institute, Chosun University School of Dentistry) ;
  • Cho, Kwang-Hee (Oral Biology Research Institute, Chosun University School of Dentistry) ;
  • Kim, Heung-Joong (Oral Biology Research Institute, Chosun University School of Dentistry)
  • Received : 2010.08.25
  • Accepted : 2010.09.03
  • Published : 2010.09.30
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Abstract

The effects of chitosan upon the experimentally induced differentiation of MDPC-23 cells, derived from mouse dental papilla cells, were investigated by RT-PCR, observations of cell morphology and Alizaline red-S staining. Chitosan was found to significantly increase and accelerate the expression of ALP mRNA but decrease the ColI transcript levels, as compared with the control, in a time-dependent manner during the differentiation of MDPC-23 cells. Chitosan also significantly downregulated ON mRNA expression and accelerated mineralization in differentiating MDPC-23 cells. These results suggest that chitosan facilitates odontoblast differentiation and mineralization and may have potential clinical applications as a dentin regeneration material.

Keywords

References

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