Journal of Animal Science and Technology

  • 제52권5호
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  • Pages.375-382
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  • 2010
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  • 2672-0191(pISSN)
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  • 2055-0391(eISSN)
한국축산학회 (Korean Society of Animal Sciences and Technology)
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체지방 감소 다클론 후보 항체가 비거세돈의 체중, 분 소화율 및 혈액 대사물질에 미치는 영향

Effects of Polyclonal Antibody Candidate to Adipocytes for Reducing Body Fat on Body Weight, Fecal Digestibility and Blood Metabolites in Pigs

  • 투고 : 2010.01.20
  • 심사 : 2010.07.22
  • 발행 : 2010.10.31
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초록

본 연구는 비거세돈 12두를 이용하여 돼지 복강 및 피하지방 감소 후보 항체를 생체 주사 처리 시 체중, 분 소화율 및 혈액 대사물질 등 영양생리대사에 미치는 영향을 조사하고자 실시하였다. Choi 등(2010)에서 개발된 돼지 복강 및 피하지방 감소 후보 항체를 피하 주사하였을 때 주사 후 4주까지 평균 체중은 수치적 증가가 발생했으나(P>0.05), 전 기간에 걸쳐 수치적 증가를 보여 항체의 처리로 인한 영향보다는 돼지의 일반적인 성장에 기인하는 것으로 판단된다. 항제 주사 처리 시 건물, 조단백질, 조지방 및 조섬유의 분 소화율은 항체 주사 전, 후 및 시험구 간의 통계적 유의성이 나타나지 않았다(P>0.05). 조회분 소화율의 경우 항체 주사 후 2주째 control에서 감소를 및 4주째 NAb에서 증가를 보였으나(P< 0.05), AAb 및 SAb에서는 유의적 변화가 없었다. 혈액 대사물질의 변화에서는 항체 주사 후 4주의 AAb 및 SAb에서 BUN 농도가 유의적으로 증가하는 것으로 나타났다(P<0.05). 하지만, 후보 항체 주사 전에서도 유사한 패턴으로 유의적 증가를 보여 항체 처리에 의한 차이보다는 개체간의 차이로 사료된다. 혈액 내 glucose 및 TG 농도는 후보 항체 처리에 의한 유의적 차이는 나타나지 않았다(P>0.05). 체지방 함량과 밀접한 관계에 있는 혈액 내 TC 농도에서는 control 대비 후보 항체 주사 후 4주차 AAb 및 SAb에서 유의적 감소가 나타나(P<0.05), 체지방 감소가 일어나고 있음을 간접적으로 추론할 수 있다. 이상의 결과를 볼 때, 본 연구에서 이용된 AAb 및 SAb는 in vivo 영양생리대사에 부정적 영향을 미치지 않는 안전한 항체로 판단된다.

Twelve pigs were used to investigate the effects of polyclonal antibody candidate against abdominal (AAb) and subcutaneous adipocyte membrane proteins (SAb) on body weight, fecaldigestibility and blood metabolites. When AAb and SAb developed by Choi et al. (2010) were injected to pigs, the numerical increase in BW (body weight) occurred at 4 weeks post-treatment, but BW for an entire period was also increased, indicating that the BW increase may not be affected by the antibodies injection. Antibodies treatment did not affect (P>0.05) fecal digestibility of dry matter, crude protein, crude fat and crude fiber. Fecal digestibility of crude ash for control (no treatment) at 2 weeks decreased, and that for non-immunized serum treatmentgroup at 4 weeks post-treatment increased, respectively (P<0.05). However, fecal digestibility of crude ash for AAb and SAb groups did not significantly change. At 4 weeks after the antibodies treatment, blood urea N concentration for AAb and SAb groups was significantly increased (P<0.05). However, these increases may not be caused by the antibodies treatment because similar pattern in blood urea N concentration occurred before the antibodies treatment. Antibodies treatment did not affect concentration of plasma glucose and triglycerides (P<0.05). Compared with control, concentration of plasma total cholesterol for AAb and SAb groups at 4 weeks post-treatment was significantly (P<0.05) decreased. This may suggest that body fat reduction possibly occurs. In conclusion, the AAb and the SAb developed by Choi et al. (2010) may have safety in nutritional physiological metabolism in pigs. Further study on in vivo fat reduction of the antibodies against abdominal and subcutaneous adipocytes of pigs should be required for fat-reduced pork production.

키워드

과제정보

연구 과제 주관 기관 : 농촌진흥청

참고문헌

  1. AOAC. 1995. Official method of analysis. 15th edition. Association of Official Analytical Chemist, Washington DC.
  2. Baek, K. H. and Choi, C. B. 2002. Effects of polyclonal antiserum against adipocyte plasma membrane proteins on body composition of passively immunized Sprague-Dawley male rats. J. Anim. Sci. & Technol. (Kor.) 44:39-44. https://doi.org/10.5187/JAST.2002.44.1.039
  3. Baek, K. H., Choi, C. W., Choi, C. B. and Lee, B. S. 2007. Passive immunization approach to reduce body fat in pigs using fat-specific polyclonal antiserum. Asian-Aust. J. Anim. Sci. 20: 1594-1599.
  4. Baek, K. H., Kwak, T. H., Oh, Y. S., Choi, C. W., Jung, K. K. and Choi, C. B. 2005. Studies on the development and utilization of polyclonal antibodies against swine adipocyte plasma membrane proteins. J. Anim. Sci. & Technol. (Kor.) 47:19-28. https://doi.org/10.5187/JAST.2005.47.1.019
  5. Choi, C. B., Lee, M. J. and Kwon, E. J. 1997. Development of polyclonal antibody to adipocyte plasma membrane proteins isolated from Korean Naive Cattle. Korean J. Anim. Sci. 39: 669-674.
  6. Choi, C. B., Lee, M. J. and Kwon, E. J. 1998. Production of polyclonal antibodies specific to procine adipocyte plasma membrane proteins in sheep. Korean J. Biomed. Lab. Sci. 4: 57-63.
  7. Choi, C. B. and Lee, S. R. 1996. Studies on the production of lean pork by immunological approach - Development of antibodies to porcine adipocyte plasma membrane. Korean J. Anim. Sci. 38:369-374.
  8. Choi, C. W., Baek, K. H., Choi, C. B., Oh, Y. K. and Hong, S. K. 2010. Development of polyclonal antibodies to abdominal and subcutaneous adipocytes for producing fat-reduced high quality pork. Korean J. Food Sci. Ani. Resour. 30(1):87-94. https://doi.org/10.5851/kosfa.2010.30.1.87
  9. Choi, C. W., Baek, K. H., Kim, S. J., Cho, S. B., Park, J. C., Kim, I. C., Kim, S. H., Ji, S. Y. and Choi, C. B. 2009a. Screening of specific protein binding to antibody against adipocyte plasma membrane proteins of pig. 한국동물자원과학회 학술발표회. PF29024. pp. 173
  10. Choi, C. W., Baek, K. H., Kim, S. J., Oh, Y. K., Hong, S. K., Kwon, E. K., Song, M. K. and Choi, C. B. 2009b. Effects of polyclonal antibodies to abdominal and subcutaneous adipocytes on ruminal fermentation patterns and blood metabolites in Korean native steers. J. Anim. Sci. & Technol. (Kor.). 51: 231-240. https://doi.org/10.5187/JAST.2009.51.3.231
  11. Choi, C. W., Kim, Y. H., Kim, S. J., Song, M. K., Kwon, E. K., Oh, Y. K., Hong, S. G. Choi, S. H. and Baek, K. H. 2008. Development of polyclonal antibodies to abdominal and subcutaneous adipocytes for fat-reduced Hanwoo beef production. Korean J. Food Sci. Ani. Resour. 28:651-659. https://doi.org/10.5851/kosfa.2008.28.5.651
  12. Cui, X. S., Kim, H. I. and Cho, S. K. 2008. Effect of the water soluble extracts from velvet antler on lipid metabolism and blood components in rats. J. Anim. Sci. & Technol. (Kor.). 50:417-428. https://doi.org/10.5187/JAST.2008.50.3.417
  13. Duncan, D. B. 1955. Multiple range and multiple F test. Biometrics. 11:1-42. https://doi.org/10.2307/3001478
  14. Early, R. J., McBride, B. W. and Ball, R. O. 1990. Growth and metabolism in somatotropin-treated steers : 1. Growth, serum chemistry and carcass weights. J. Anim. Sci. 68:4134-4143.
  15. Enright, W. J., Quirke, J. F., Gluckman, P. D., Breier, B. H., Kennedy, L. G., Hart, I. C., Rochecoert, J. F. and Allen, P. 1990. Effects of long-time administration of pituitary-derived bovine growth hormone and estradiol on growth in steers. J. Anim. Sci. 68:2345-2356.
  16. Flint, D. J., Coggrave, H., Futter, C. E., Gardner, M. J. and Clarke, D. J. 1986. Stimulatory and cytotoxic effects of an antiserum to adipocyte plasma membranes on adipose tissue metabolism in vitro and in vivo. Int. J. Obes. 10:69-77.
  17. Flint, D. J. 1998. Effects of antibodies to adipocytes on body weight, food intake, and adipose tissue cellularity in obese rats. Biochem. Biophys. Res. Commun. 252:263-268. https://doi.org/10.1006/bbrc.1998.9622
  18. Futter, C. E. and Flint, D. J. 1987. Long-term reduction of adiposity in rats after passive immunization with antibodies to rat fat cell plasma membranes. In: E. M. Berry, S. H. Blondheim, H. E. Eliahon and E. Shafrir (Ed.). Recent Advances in obesity research: V. in Proc. 5th Int. Congr. on Obesity, Sept. 14-19, 1986, Jerusalem, Israel. p181. John Libbey and Company, London.
  19. Futter, C. E., Panton, D., Kestin, S. and Flint, D. J. 1992. Mechanism of action of cytotoxic antibodies to adipocytes on adipose tissue, liver, and food intake in the rat. Int. J. Obes. Relat. Metab. Disord. 16(8):615-622.
  20. Hong, T. T. and Lindberg, J. E. 2007. Effect of guar gum on nitrogen secretion into isolated loops of jejunum in conscious growing pigs. Br. J. Nutr. 52:499-505. https://doi.org/10.1079/BJN19840117
  21. Kestin, S., Kennedy, R., Tonner, E., Kiernan, M., Cryer, A., Griffin, H., Butterwith, S., Rhind, S. and Flint, D. J. 1993. Decreased fat content and increased lean in pigs treated with antibodies to adipocyte plasma membranes. J. Anim. Sci. 71, 1486-1494.
  22. Kim, H. J., Min, B. J., Cho, J. H., Chen, Y. J. Yoo, J. S., Kim, I. H., Jang J. S. and Lee, Y. K. 2006. Effects of mud flat bacteria origin protease supplementation on growth performance, amino acid digestibility, blood characteristics, meat quality, fecal VFA and $NH_3$-N concentration in finishing pigs. J. Anim. Sci. & Technol. (Kor.) 48:49-58. https://doi.org/10.5187/JAST.2006.48.1.049
  23. Kook, K., Jeong, J. H. and Kim, K. H. 2005. The effects of supplemental levels of bamboo vinegar liquids on growth performance, serum profile, carcass grade, and meat quality characteristics in finishing pigs. J. Anim. Sci. & Technol. (Kor.) 47:721-730. https://doi.org/10.5187/JAST.2005.47.5.721
  24. Kwon, E. G., Hong, S. K., Seong, H. H., Yun, S. G., Park, B. K., Cho, Y. M., Cho, W. M., Chang, S. S., Shin, K. J. and Paek, B. H. 2005. Effects of ad libitum and restricted feeding of concentrates on body weight gain, feed intake and blood metabolites of Hanwoo steers at various growth stages. J. Anim. Sci. & Technol. (Kor.) 47:745-758. https://doi.org/10.5187/JAST.2005.47.5.745
  25. Moloney, A. P. 1995. Immunomodulation of fat deposition. Livest. Prod. Sci. 42:239-245. https://doi.org/10.1016/0301-6226(95)00026-H
  26. Moloney, A. P., Allen, P. and Enright, W. J. 1998. Passive immunizationof sheep against adipose tissue : effects on metabolism, growth and body composition. Livest. Prod. Sci. 56:233-244. https://doi.org/10.1016/S0301-6226(98)00152-3
  27. Moloney, A. P., Moore, W. and Allen, P. 1994. Decreasing body fat by passive immunisation against adipose tissue. J. Anim. Sci. 72 (suppl. 1):364.
  28. Moloney, A. P., Allen, P. and Enright, W. J. 2002. Body composition and adipose tissue accretion in lambs passively immunised against adipose tissue. Livest. Prod. Sci. 74:165-174. https://doi.org/10.1016/S0301-6226(01)00299-8
  29. Panton, D., Futter, C., Kestin, S. and Flint, D. 1990. Increased growth and protein deposition in rats treated with antibodies to adipocytes. Am. J. Physiol. 258:E985-9.
  30. Park, J. C., Kim, Y. H., Jung, H. J., Lee, S. D., Jang, H. D., Kim, I. C., Lee, S. J., Lee, J. J., Lee. C. H. and Lee, S. S. 2006. Effects of dietary supplementation of cultured wild-ginseng powder or its fermented culture byproducts on growth performance and carcass parameters in finishing pigs. J. Anim. Sci. & Technol. (Kor.) 48:819-826. https://doi.org/10.5187/JAST.2006.48.6.819
  31. Raghuvansi, S., Tripathi, M., Mishra, A., Chaturvedi, O., Prasad, R., Saraswat, B. and Jakhmola, R. 2006. Feed digestion, rumen fermentation and blood biochemical constituents in Malpura rams fed a complete feed-block diet with the inclusion of tree leaves. Small Rumin. Res. 71:21-30. https://doi.org/10.1016/j.smallrumres.2006.03.012
  32. SAS. 2002. SAS User's Guide, Statistics, Version 9.1 Ed. SAS Inst., Inc., Cary, NC.
  33. Sauer, W. C., Mosenthin, R., Ahrens, F. and den Hartog, L. A. 1991. The effect of source of fiber on idea and fecal amino acid digestibility and bacterial nitrogen excretion in growing pigs. J. Anim. Sci. 69:4070-4077.
  34. Scheuerermann, S. E. 1993. Effect of the probiotic Paciflor (CIP5832) on energy and protein metabolism of growing pig. Anim. Feed Sci. Technol. 41:181-186. https://doi.org/10.1016/0377-8401(93)90011-8
  35. Wheeler, T. L., Davis, G. W., Stoecker, B. J. and Harmon, C. J. 1987. Cholesterol concentration of longissimus muscle, subcutaneous fat and serum of two beef cattle breed types. J. Anim. Sci. 65:1531-1537.