DOI QR코드

DOI QR Code

Rosa multiflora Thunberg fruit 추출물로부터 Helicobacter pylori 억제물질의 분리 및 동정

Isolation and Identification of Inhibitory Compounds on Helicobacter pylori from Rosa multiflora Thunberg Fruit Extracts

  • 박기태 (경북대학교 식품공학과) ;
  • 김진성 (경북대학교 식품공학과) ;
  • 조분성 (경북대학교 식품공학과) ;
  • 안봉전 (대구한의대학교 화장품약리학과) ;
  • 천성숙 (엔아이피 바이오텍) ;
  • 김정환 (엔아이피 바이오텍) ;
  • 조영제 (경북대학교 응용생명과학부)
  • 투고 : 2010.07.14
  • 심사 : 2010.10.06
  • 발행 : 2010.10.30

초록

영실에 존재하는 페놀성 화합물의 추출 최적 조건을 알아보고자 추출용매를 달리하여 phenol성 물질의 함량을 비교한 결과, 70% ethanol을 용매로 하여 24시간 교반 추출하였을 때 phenol성 물질의 용출량이 13.8 mg/g 이상으로 가장 높았다. Helicobacter pylori에 대한 항균효과는 영실추출물을 150 ${\mu}g$/ml의 농도로 첨가하였을 때 생육 저해환이 12 mm, 200 ${\mu}g$/ml 일 때 14 mm의 생육저해환이 나타나 항균활성이 우수한 것을 알 수 있었다. H. pylori에 대한 저해물질을 정제하기 위하여 영실을 70% 에탄올로 추출한 후 Sephadex LH-20 column과 $C_{18}$ cartridge column을 이용하여 항균활성을 가지는 물질을 순수 정제하고 구조를 동정한 결과 protocatechuic acid, chlorogenic acid 및 quercetin으로 동정하였으며, 단일물질에 의한 H. pylori에 대한 저해효과 보다 혼합물질에 의한 synergy 효과에 의해 저해 효과가 높아지는 것을 알 수 있었다.

The antimicrobial activity of 70% ethanol extracts from Rosa multiflora Thunberg fruit against Helicobacter pylori was examined. The inhibitory activity of Rosa multiflora Thunberg fruit extracts against H. pylori was determined to clear a zone of 14 mm with 70% ethanol extracts. Purification of inhibitory compounds was carried on Sephadex LH-20 and $C_{18}$ cartridge column chromatography using a gradient procedure, with increasing ethanol ($0{\rightarrow}100%$) in $H_2O$. The chemical structure of the purified inhibitory compounds on H. pylori was identified to be protocatechuic acid, chlorogenic acid and quercetin by FAB-MS, NMR and IR spectrum.

키워드

참고문헌

  1. Azuma, K., M. Nakayama, M. Koshika, K. Lppoushi, Y. Yamaguchi, K. Kohata, Y. Yamauchi, H. Ito, and H. Higashio. 1999. Phenolic antioxidants from the leaves of Corchorus olitorius L. J. Agric. Food Chem. 47, 3963-3966. https://doi.org/10.1021/jf990347p
  2. Barnes, H. M., J. R. Reldman, and W. V. White. 1950. Isochlorogenic acid. Isolation from coffee and structure studies. J. Am. Chem. Soc. 72, 4178-4183. https://doi.org/10.1021/ja01165a095
  3. Bunney, S. 1992. The Illustrated encyclopedia of herbs. Chancellor Press. London, England.
  4. Cho, Y. J., I. S. Ju, O. J. Kwon, S. S. Chun, B. J. An, and J. H. Kim. 2008. Biological and antimicrobial activity of Portulaca oleracea. J. Korean Soc. Appl. Biol. Chem. 51, 49-54.
  5. Cho, Y. S., S. S. Chun, H. J. Kwon, S. J. Yoon, and K. H. Lee. 2005. Comparison of physiological activities between hot-water and ethanol extracts of Bokbunja (Rubus coreanum F.). J. Korean Soc. Food Sci. Nutr. 34, 790-796. https://doi.org/10.3746/jkfn.2005.34.6.790
  6. Choi, H. R. and E. H. Choi. 2003. Screening of antimicrobial and antioxidative herb. J. Natural Science 15, 123-131.
  7. Chun, S. S., D. A. Vattem, Y. T. Lin, and K. Shetty. 2005. Phenolic antioxidants from clonal oregano (Origanum vulgare) with antimicrobial activity against Helicobacter pylori. Process Biochemistry 40, 809-816. https://doi.org/10.1016/j.procbio.2004.02.018
  8. Clark, A. M. and F. S. Tl-Feraly. 1981. Antimicrobial activity of phenolic constituents of Magnolia grandiflora L. J. Pharm. Sci. 70, 951-952. https://doi.org/10.1002/jps.2600700833
  9. Folin, O. and W. Denis. 1912. On phosphotungastic-phosphomolybetic compounds as color reagents. J. Biol. Chem. 12, 239-249.
  10. Goodwin, C. S., J. A. Armstrong, T. Chilvers, M. Peters, D. Collins, L. Sly, W. MacConnell, and W. E. S. Harper. 1989. Transfer of Campylobacter pylori and Campylobacter mustelae to Helicobacter gen. nov. as Helicobacter pylori comb. nov. and Helicobacter mustelue comb. nov. respectively. Int. J. Syst. Bacteriol. 39, 397-402. https://doi.org/10.1099/00207713-39-4-397
  11. Ham, S. S., J. K. Hong, and J. H. Lee. 1997. Antimutagenic effects of juices from edible Korean wild herbs. J. Food Sci. Nutr. 2. 155-161.
  12. Huang, M. T., C. T. Ho, and C. Y. Lee. 1992. Phenolic compounds in food. In phenolic compounds in food and their effects on health II. pp. 2-7, Maple Press, New York.
  13. Hubue, G., V. Wray, and A. Nahrstedt. 1999. Flavonol Oligosaccharides from the Seeds of Aesculus Hippocastanum. Planta Med. 65, 638-640.
  14. Institute of Natural Product in Seoul National University. 2003. Treatise on asian herbal medicines, Daewon, Seoul, Korea.
  15. Irwin, B. Y. and A. Pearl. 1946. Reactions of vaillin and its derived compounds. Ⅳ, The Caustic fusion of vaillin. J. Am. Chem. Soc. 68, 2180-2184. https://doi.org/10.1021/ja01215a021
  16. Jones, D. M. and A. Curry. 1990. The genesis of coccal forms of Helicobacter pylori, pp. 29, in Helicobacter pylori gastritis and peptic ulcer. In Malfertheiner, P. and H. Ditschumeit. (eds.), Springer-Verlh, Berlin.
  17. Masakazu, A. 1962. Components of the flower petals of Rosa multiflora and Rubus hirsutus. Yakugaku Zasshi 82, 771-773.
  18. Moon T. C., J. O. Park, K. W. Chung, K. H. Son, H. P. Kim, S. S. Kang, and H. W. Chang. 1999. Anti-inflammatory activity of the flavonoid components of Lonicera japonica. Yakhak Hoeji 43, 117-123.
  19. Park, C. K., H. J. Choi, H. S. Youn, W. K. Lee, M. J. Cho, K. H. Kang, S. C. Baik, and K. H. Rhee. 1994. Chemotherapy of Helicobacter pylori infection. J. Korean Soc. Microbiol. 80, 667-672.
  20. Park, J. C., J. M. Hur, and J. G. Park. 2002. Biological activities of Umbelliferae family plants and their bioactive flavonoids. Food Industry and Nutrition 7, 30-34.
  21. Park, J. H. 2002. Encyclopedia of Chinese crude drugs. pp. 557.
  22. Rauws, E. A., W. Langenberg, J. Houthoff, and D. Moore-jones. 1990. Is doxycycline more effective than tetracycline HCI in triple therapy of Helicobacter pylori. Gastroenterol. 98, 24-27.
  23. Rhee, K. H., H. S. Youn, S. C. Baik, W. K. Lee, M. J. Cho, H. J. Choi, K. Y. Maeng, and K. W. Ko. 1990. Prevalence of Helicobacter pylori infection in Korea. J. Korean Soc. Microbiol. 25, 475-490.
  24. Ryoo, J. W. and B. C. Cha. 1998. Mineral content and antioxidative activity in some herb plant. Korean J. Medicinal Crop Sci. 6, 28-32.
  25. Steer, H. W. 1975. Ultraststructure of cell migration through the gastric epithelium and its relationship to bacteria. J. Clin. Path. 28, 639-646. https://doi.org/10.1136/jcp.28.8.639
  26. Suerbaum, S. and P. Michetri. 2002. Helicobacter pylori infection. N. Engl. J. Med. 347, 1175-1186. https://doi.org/10.1056/NEJMra020542

피인용 문헌

  1. Evaluation of In Vivo Safety of Inhibitory Compounds from Cheongmoknosang Mulberry Leaves against Helicobacter pylori vol.40, pp.10, 2011, https://doi.org/10.3746/jkfn.2011.40.10.1404
  2. Evaluation of Medicinal Activity on Isolated Inhibitory Compounds against Helicobacter pylori from Cheongmoknosang Mulberry Leaves vol.54, pp.4, 2011, https://doi.org/10.3839/jabc.2011.043
  3. Inhibitory Activity against Helicobacter pylori of Isolated Compounds from Pinus koraiensis Siebold et Zucc Leaves vol.59, pp.1, 2016, https://doi.org/10.3839/jabc.2016.005