Development and Reproduction (한국발생생물학회지:발생과생식)

The Korean Society of Developmental Biology (한국발생생물학회)
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Current Progress and Prospects of Reprogramming Factors - Stem Cells vs Germ Cells -

줄기세포와 생식세포에서 리프로그래밍 인자에 대한 최근 연구 동향과 전망

  • Seo, You-Mi (Dept. of Biomedical Science, College of Life Science, CHA University) ;
  • Lee, Kyung-Ah (Dept. of Biomedical Science, College of Life Science, CHA University)
  • 서유미 (차의과학대학교 의생명과학과) ;
  • 이경아 (차의과학대학교 의생명과학과)
  • Received : 2010.02.17
  • Accepted : 2010.04.15
  • Published : 2010.06.30
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Abstract

Recently induced pluripotent stem (iPS) cells are derived from somatic cells by ectopic expression of several transcription factors (reprogramming factors) using technology of somatic cell reprogramming. iPS cells are able to selfrenew and differentiate into all type of cells in the body similarly to embryonic stem cells. Because iPS cells have advantages that can avoid immune rejection after transplantation and ethical issues unlike embryonic stem cells, research on iPS has made significant progress since the first report by Yamanaka in 2006. Nevertheless of many advantages of iPS, safer methods to introduce reprogramming factors into somatic cells must be developed due to safety concerns regarding viral vectors, and safer reprogramming factors to substitute the oncogenes should be evaluated for clinical application of iPS. Here we discuss the recent progress in reprogramming factors in embryonic stem cells, oocytes, and embryos, and discuss further research for finding new, more reliable and safer reprogramming factors.

최근에 체세포 리프로그래밍 기법을 사용하여 체세포에 몇 가지 전사인자(리프로그래밍 인자)를 넣어줌으로써 유도만능줄기세포(induced pluripotent stem cell, iPS)를 만드는데 성공하였다. 유도만능줄기세포는 배아줄기세포와 유사하게 자가재생 할 수 있는 능력이 있으며, 신체의 모든 타입의 세포로 분화할 수 있는 특징을 가지고 있다. 배아줄기세포와는 달리 면역거부반응이 없다는 점과 윤리적인 문제로부터 자유롭다는 장점이 있어 2006년 Yamanaka 팀이 유도만능줄기세포에 관해 처음 보고한 이후로 이 분야 연구의 급속한 발전이 이루어지고 있다. 하지만 안전성의 문제점 때문에 세포치료제로 사용되기 위해서는 리프로그래밍 인자의 도입 방법 및 새로운 리프로그래밍 인자의 발굴 등 몇 가지 해결해야 할 점들이 남아 있다. 본 종설에서는 유도만능줄기세포를 만드는데 사용된 몇 가지 리프로그래밍 인자에 대해 보고된 연구 내용을 리프로그래밍 인자가 존재하는 세포인 배아줄기세포 및 난자와 배아에서 정리하고자 하며, 리프로그래밍 인자의 연구에 관한 방향에 대해 논의하고자 한다.

Keywords

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