The Korean Journal of Pesticide Science (농약과학회지)

The Korean Society of Pesticide Science (한국농약과학회)
권호 표지

Control Effects of Micromonospora sp. AW050027 by Media Optimization and Microbial Treatment Against Pine Wood Nematode

Micromonospora sp. AW050027 균주의 배지최적화 및 미생물제제 처리에 의한 소나무재선충 방제효과

  • Park, Dong-Jin (Microbiological Resource Center, Korea Research Institute of Bioscience and Biotechnology) ;
  • Lee, Jae-Chan (Microbiological Resource Center, Korea Research Institute of Bioscience and Biotechnology) ;
  • Chang, Yong-Ha (Microbiological Resource Center, Korea Research Institute of Bioscience and Biotechnology) ;
  • Kim, Chang-Jin (Microbiological Resource Center, Korea Research Institute of Bioscience and Biotechnology)
  • 박동진 (한국생명공학연구원 미생물자원센터) ;
  • 이재찬 (한국생명공학연구원 미생물자원센터) ;
  • 장용하 (한국생명공학연구원 미생물자원센터) ;
  • 김창진 (한국생명공학연구원 미생물자원센터)
  • Received : 2010.04.23
  • Accepted : 2010.05.11
  • Published : 2010.06.30
Download PDF
⟨ Previous Next ⟩

Abstract

Nematicidal activity against pine wood nematode, Bursaphelenchus xylophilus, was tested in the pot and field by the treatment of microbial formulation after media optimization. The optimized media composition was glycerol 10 g, soybean meal 10 g, NaCl 1 g, $CaCO_3$ 2 g, $K_2HPO_4$ 0.125 g per liter and microbial complex formulation was made with liquid and powder type. Most effective antibiotics against symbiotic microorganism with nematode, kanamycin, was added to the formulation. The control effects against pine wood nematodes were checked by pot test and field test. In the result of treatment by trunk injection, five times treatment was more effective than one time and the treatment with the formulation of concentrated culture supernatant was the most effective in the nematicidal activity showing below 10% mortality in pine tree.

소나무재선충에 대한 살선충 활성을 나타내는 Micromonospora sp. AW050027 균주를 최적의 배지에서 배양한 미생물배양액을 제제하여 포트 및 포장에서 살선충 효과를 확인하였다. 최적화된 배지조성은 리터당 glycerol 10 g, soybean meal 10 g, NaCl 1 g, $CaCO_3$ 2 g, $K_2HPO_4$ 0.125 g 이었으며, 활성균주를 배양하여 농축한 후 액상 및 분말 미생물제제를 제조하였다. 제제에는 소나무재선충 공생미생물에 대하여 가장 효과적으로 항균활성을 나타내는 kanamycin을 첨가하여 사용하였다. 포트시험, 예비포장 시험 및 본포장 시험을 통하여 소나무재선충 방제효과를 확인한 결과, 액상제제를 수간수사하여 처리한 경우 1회처리시 고사율 53%, 5회 분할처리시 28%로 분할처리가 1회처리보다 2배 가량 효과적이었고, 배양농축액을 분할처리한 경우에서는 대조구의 고사율 100%에 대하여 고사율 10% 이내로 가장 낮게 나타났다.

Keywords

References

  1. Edward, O. R. and M. J. Linit (1992) Transmission of Bursaphelenchus xylophilus through oviposition wounds of Monachamus alternatus (Coleoptera : Cerambycidae). J. Nematol. 24:133-139.
  2. Evans, H. F., D. G. McNamara, H. Braash, J. Chadoeuf and C. Magnusson (1996) Pest risk analysis (PRA) for the territories of the European Union (as PRA area) on Bursaphelenchus xylophilus and its vectors in the genus Monochamus. OEPP IEPPO Bull. 26:199-249. https://doi.org/10.1111/j.1365-2338.1996.tb00594.x
  3. Han, Z. M., Y. D. Hong and B. G. Zhao (2003) A study on pathogenicity of bacteria carried by pine wood nematodes. J. Phytopathol. 150:683-689.
  4. Kawazu, K., H. Zhang, H. Tamashita and H. Kanzaki (1996)Relationship between the pathogenicity of the pine wood nematode, Bursaphelenchus xylophilus, and phenylacetic acid production. Biosci. Biotechnol. Biochem. 60:1430-1415. https://doi.org/10.1271/bbb.60.1430
  5. Kishi, Y. (1995) The pine wood nematode and the Japanese pine sawyer. Thomas Company Limited, Tokyo, Japan. pp. 302.
  6. Kwon, T. S. (2008) Change of abundance of arthropods in pine forests caused by aerial insecticide spray. Arch. Environ. Contam. Toxicol. 54:92-106. https://doi.org/10.1007/s00244-007-9013-5
  7. Mamiya, Y. and N. Enda (1972) Transmission of Bursaphelenchus lignicolus (Nematoda : Aphelenchoididae) by Monachamus alternatus (Coleoptera : Cerambycidae). Nematologica 18:159-162. https://doi.org/10.1163/187529272X00395
  8. Morimoto, K. and A. Iwasaki (1972) Role of Monachamus alternatus (Coleoptera : Cerambycidae) as a vector of Bursaphelenchus lignicolus (Nematoda : Aphelenchoididae). J. Jap. For. Soc. 54:177-183.
  9. Sato, H., T. Sakuyama and M. Kobayashi (1987) Transmission of Bursaphelenchus xylophilus (Steiner et Buhrer) Nickle (Nematode, Aphelenchoididae) by Monochamus saltuarius (Gebler) (Coleoptera, Cerambycidae). J. Jpn. For. Soc. 69: 492-496.
  10. Suzuki, K (2002) Pine wilt disease-a threat to pine forest in Europe. Dendrobiol. 48:71-74.
  11. Wingfield, M. J. and R. B. Blanchette (1983) The pine-wood nematode, Bursaphelenchus xylophilus, in Minnesota and Wisconsin: insect associates and transmission studies. Can. J. For. Res. 13:1068-1076. https://doi.org/10.1139/x83-143
  12. Yano, M. (1913) Investigations on the cause of pine mortality in Nagasaki prefecture. Sanrin-Koho. 4:1-14.
  13. Zao, B. G. and F. Lin (2005) Mutualistic symbiosis between Bursaphelenchus xylophilus and bacteria of genus Pseudomonas. For. Path. 35:339-345. https://doi.org/10.1111/j.1439-0329.2005.00417.x
  14. Zao, B. G., Y. Lin and F. Lin (2007) Effects of bacteria associated with pine wood nematode (Bursaphelenchus xylophilus) on development and egg production of the nematode. J. Phytopathol. 155:26-30. https://doi.org/10.1111/j.1439-0434.2006.01188.x
  15. 문일성, 이상명, 박지두, 여운홍 (1995) 소나무재선충(Bursaphelenchus xylophilus)과 매개충인 솔수염하늘소(Monochamus alternatus)의 분포 및 방제. 산림과학논문집 51:119-126.
  16. 박동진, 이재찬, 김판경, 김창진 (2008) 소나무재선충에 대한 살선충 활성을 나타내는 Micromonospora sp.의 분리 및 동정. 농약과학회지 12(1):97-101.
  17. 산림청, 국립산림과학원 (2006) 소나무재선충병. 산림과학속보. '06-13.
  18. 산림청, 국립산림과학원 (2007) 잣나무림에서의 소나무재선충병 피 해특성 및 방제전략. 산림과학속보. '07-01.
  19. 안희근, 김민기, 양정오, 노두진, 강신호, 김길하 (2008) 북방수염하늘소의 Ipsenol, Ipsdienol에 대한 유인효과. 농약과학회지 12 (4):391-396.
  20. 이상명, 김동수, 김철수, 추호렬, 이동운 (2008) 곰솔(Pinus thunbergii)에서 Abametin과 Emamectin benzoate를 이용한 소나 무재선충과 솔잎혹파리 및 솔껍질깍지벌레의 동시방제 가능성.농약과학회지 Sci. 12(4):363-367.
  21. 이상명, 문일성, 정영진, 이상길, 이동운, 후호열, 박정규 (2003) 솔수염하늘소(Monochamus alternatus)에 대한 수종 살충제의 살충효과. 한국임학회지 92(4):305-312.
  22. 최영연, 문일성 (1989) 한국에 있어서 소나무재선(Bursaphelenchus xylophilus)의 분포와 병원성. 한국식물병리학회지 5:277-286.