Bulletin of the Korean Chemical Society

Korean Chemical Society (대한화학회)
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Performance Characteristic of the β-Human Chorionic Gonadotropin Assay on the Access 2 for Its Use in Doping Control

  • Kang, Min-Jung (Integrated Omics Center, Korea Institute of Science and Technology) ;
  • Lee, Hye-Im (Doping control center, Korea Institute of Science and Technology) ;
  • Shin, Sang-Mi (Doping control center, Korea Institute of Science and Technology) ;
  • Yoo, Hye-Hyun (Department of Pharmacy, College of Pharmacy, Hanyang University) ;
  • Kwon, Oh-Seung (Doping control center, Korea Institute of Science and Technology) ;
  • Jin, Chang-Bae (Integrated Omics Center, Korea Institute of Science and Technology) ;
  • Chung, Bong-Chul (Integrated Omics Center, Korea Institute of Science and Technology)
  • Received : 2010.07.21
  • Accepted : 2010.08.23
  • Published : 2010.10.20
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Abstract

The objective of this study was to validate performance characteristics of the Access 2 (Beckman coulter) system for hCG assays for use as a confirmation test for doping control. The Access 2 assay was linear up to 500 IU/L. The correlation coefficient was higher than 0.999, and the sensitivity of the linearity was 0.82. There were no false positive or false negative cases. LOD was 0.59 IU/L. The method was robust when performed by different people. Repeatability and reproducibility were below 7%. We compared reproducibility and recoveries of Access 2 and Elecsys 2010. Access 2 demonstrated higher reproducibility than Elecsys 2010. Recoveries (accuracy) of Access 2 were between 85 and 105%. Recoveries for Elecsys 2010 were between 91 and 104%.

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