Journal of Periodontal and Implant Science

Korean Academy of Periodontology (대한치주과학회)
권호 표지
DOI QR코드

DOI QR Code

Bone regeneration effects of human allogenous bone substitutes: a preliminary study

  • Lee, Deok-Won (Department of Periodontology and Dental Research Institute, Seoul National University College of Dentistry) ;
  • Koo, Ki-Tae (Department of Periodontology and Dental Research Institute, Seoul National University College of Dentistry) ;
  • Seol, Yang-Jo (Department of Periodontology and Dental Research Institute, Seoul National University College of Dentistry) ;
  • Lee, Yong-Moo (Department of Periodontology and Dental Research Institute, Seoul National University College of Dentistry) ;
  • Ku, Young (Department of Periodontology and Dental Research Institute, Seoul National University College of Dentistry) ;
  • Rhyu, In-Chul (Department of Periodontology and Dental Research Institute, Seoul National University College of Dentistry) ;
  • Chung, Chong-Pyoung (Department of Periodontology and Dental Research Institute, Seoul National University College of Dentistry) ;
  • Kim, Tae-Il (Department of Periodontology and Dental Research Institute, Seoul National University College of Dentistry)
  • Received : 2010.04.26
  • Accepted : 2010.06.01
  • Published : 2010.06.30
Download PDF
⟨ Previous Next ⟩

Abstract

Purpose: The purpose of this study was to compare the bone regeneration effects of cortical, cancellous, and cortico-cancellous human bone substitutes on calvarial defects of rabbits. Methods: Four 8-mm diameter calvarial defects were created in each of nine New Zealand white rabbits. Freeze-dried cortical bone, freeze-dried cortico-cancellous bone, and demineralized bone matrix with freeze-dried cancellous bone were inserted into the defects, while the non-grafted defect was regarded as the control. After 4, 8, and 12 weeks of healing, the experimental animals were euthanized for specimen preparation. Micro-computed tomography (micro-CT) was performed to calculate the percent bone volume. After histological evaluation, histomorphometric analysis was performed to quantify new bone formation. Results: In micro-CT evaluation, freeze-dried cortico-cancellous human bone showed the highest percent bone volume value among the experimental groups at week 4. At week 8 and week 12, freeze-dried cortical human bone showed the highest percent bone volume value among the experimental groups. In histologic evaluation, at week 4, freeze-dried cortico-cancellous human bone showed more prominent osteoid tissue than any other group. New bone formation was increased in all of the experimental groups at week 8 and 12. Histomorphometric data showed that freeze-dried cortico-cancellous human bone showed a significantly higher new bone formation percentile value than any other experimental group at week 4. At week 8, freeze-dried cortical human bone showed the highest value, of which a significant difference existed between freeze-dried cortical human bone and demineralized bone matrix with freeze-dried cancellous human bone. At week 12, there were no significant differences among the experimental groups. Conclusions: Freeze-dried cortico-cancellous human bone showed swift new bone formation at the 4-week healing phase, whereas there was less difference in new bone formation among the experimental groups in the following healing phases.

Keywords

References

  1. Helm GA, Dayoub H, Jane JA Jr. Bone graft substitutes for the promotion of spinal arthrodesis. Neurosurg Focus 2001;10:E4.
  2. Giannoudis PV, Dinopoulos H, Tsiridis E. Bone substitutes: an update. Injury 2005;36 Suppl 3:S20-7.
  3. Urist MR. Bone: formation by autoinduction. Science 1965;150:893-9. https://doi.org/10.1126/science.150.3698.893
  4. Misch CE, Dietsh F. Bone-grafting materials in implant dentistry. Implant Dent 1993;2:158-67. https://doi.org/10.1097/00008505-199309000-00003
  5. Pietrzak WS, Woodell-May J, McDonald N. Assay of bone morphogenetic protein-2, -4, and -7 in human demineralized bone matrix. J Craniofac Surg 2006;17:84-90. https://doi.org/10.1097/01.scs.0000179745.91165.73
  6. Mellonig JT, Bowers GM, Bailey RC. Comparison of bone graft materials. Part I. New bone formation with autografts and allografts determined by Strontium-85. J Periodontol 1981;52:291-6. https://doi.org/10.1902/jop.1981.52.6.291
  7. Mellonig JT, Bowers GM, Cotton WR. Comparison of bone graft materials. Part II. New bone formation with autografts and allografts: a histological evaluation. J Periodontol 1981;52:297-302. https://doi.org/10.1902/jop.1981.52.6.297
  8. Piattelli A, Scarano A, Corigliano M, Piattelli M. Comparison of bone regeneration with the use of mineralized and demineralized freeze-dried bone allografts: a histological and histochemical study in man. Biomaterials 1996;17:1127-31. https://doi.org/10.1016/0142-9612(96)85915-1
  9. Rummelhart JM, Mellonig JT, Gray JL, Towle HJ. A comparison of freeze-dried bone allograft and demineralized freeze-dried bone allograft in human periodontal osseous defects. J Periodontol 1989;60:655-63. https://doi.org/10.1902/jop.1989.60.12.655
  10. Mellonig JT, Bowers GM, Bright RW, Lawrence JJ. Clinical evaluation of freeze-dried bone allografts in periodontal osseous defects. J Periodontol 1976;47:125-31. https://doi.org/10.1902/jop.1976.47.3.125
  11. Cammack GV 2nd, Nevins M, Clem DS 3rd, Hatch JP, Mellonig JT. Histologic evaluation of mineralized and demineralized freeze-dried bone allograft for ridge and sinus augmentations. Int J Periodontics Restorative Dent 2005;25:231-7.
  12. Cornell CN. Osteobiologics. Bull Hosp Jt Dis 2004;62:13-7.
  13. Behairy Y, Jasty M. Bone grafts and bone substitutes in hip and knee surgery. Orthop Clin North Am 1999;30:661-71. https://doi.org/10.1016/S0030-5898(05)70118-8
  14. Goldberg VM, Stevenson S. Natural history of autografts and allografts. Clin Orthop Relat Res 1987:7-16.
  15. Ludwig SC, Boden SD. Osteoinductive bone graft substitutes for spinal fusion: a basic science summary. Orthop Clin North Am 1999;30:635-45. https://doi.org/10.1016/S0030-5898(05)70116-4
  16. Schreurs BW, Slooff TJ, Buma P, Gardeniers JW, Huiskes R. Acetabular reconstruction with impacted morsellised cancellous bone graft and cement. A 10- to 15-year follow-up of 60 revision arthroplasties. J Bone Joint Surg Br 1998;80:391-5. https://doi.org/10.1302/0301-620X.80B3.8534

Cited by

  1. Solid Free‐Form Fabrication of Tissue‐Engineering Scaffolds with a Poly(lactic‐co‐glycolic acid) Grafted Hyaluronic Acid Conjugate Encapsulating and Intact Bone Morphogenetic P vol.21, pp.15, 2010, https://doi.org/10.1002/adfm.201100612
  2. Comparison of bone regeneration using three demineralized freeze-dried bone allografts: A histological and histomorphometric study in rabbit calvaria vol.9, pp.5, 2010, https://doi.org/10.4103/1735-3327.104873
  3. Graft incorporation and implant osseointegration following the use of autologous and fresh‐frozen allogeneic block bone grafts for lateral ridge augmentation vol.25, pp.2, 2010, https://doi.org/10.1111/clr.12107
  4. Iliac Crest Fresh-Frozen Allografts Versus Autografts in Oral Pre-Prosthetic Bone Reconstructive Surgery : Histologic and Histomorphometric Study vol.25, pp.6, 2010, https://doi.org/10.1097/id.0000000000000451
  5. Histologic and histomorphometric evaluation of bone regeneration using nanocrystalline hydroxyapatite and human freeze-dried bone graft : An experimental study in rabbit vol.78, pp.2, 2010, https://doi.org/10.1007/s00056-016-0067-8
  6. Osteoimmunomodulation, osseointegration, andin vivomechanical integrity of pure Mg coated with HA nanorod/pore-sealed MgO bilayer vol.6, pp.12, 2010, https://doi.org/10.1039/c8bm00901e
  7. A study of bone regeneration effect according to the two different graft bone materials in the cranial defects of rabbits vol.42, pp.4, 2010, https://doi.org/10.21851/obr.42.04.201812.198