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Elucidation of Mode of Action of Pantoea agglomerans 59-4 for Controlling Garlic Blue Mold

마늘 푸른곰팡이병 방제용 Pantoea agglomerans 59-4의 억제기작 해석

  • Kim, Yong-Ki (Department of Organic Agriculture, Division of Agro-Food Safety, NAAS, RDA) ;
  • Kwon, Mi-Kyung (Department of Organic Agriculture, Division of Agro-Food Safety, NAAS, RDA) ;
  • Yeh, Wan-Hae (Department of Organic Agriculture, Division of Agro-Food Safety, NAAS, RDA) ;
  • Hong, Sung-Jun (Department of Organic Agriculture, Division of Agro-Food Safety, NAAS, RDA) ;
  • Jee, Hyung-Jin (Department of Organic Agriculture, Division of Agro-Food Safety, NAAS, RDA) ;
  • Park, Jong-Ho (Department of Organic Agriculture, Division of Agro-Food Safety, NAAS, RDA) ;
  • Han, Eun-Jung (Department of Organic Agriculture, Division of Agro-Food Safety, NAAS, RDA) ;
  • Park, Kyung-Seok (Department of Agricultural Microbiology, Division of Agricultural Biology, NAAS, RDA) ;
  • Lee, Sang-Yeob (Department of Agricultural Microbiology, Division of Agricultural Biology, NAAS, RDA) ;
  • Lee, Seong-Don (Department of Research Coordination, Division of Research Policy Bureau, RDA)
  • 김용기 (국립농업과학원 농산물안전성부 유기농업과) ;
  • 권미경 (국립농업과학원 농산물안전성부 유기농업과) ;
  • 예완해 (국립농업과학원 농산물안전성부 유기농업과) ;
  • 홍성준 (국립농업과학원 농산물안전성부 유기농업과) ;
  • 지형진 (국립농업과학원 농산물안전성부 유기농업과) ;
  • 박종호 (국립농업과학원 농산물안전성부 유기농업과) ;
  • 한은정 (국립농업과학원 농산물안전성부 유기농업과) ;
  • 박경석 (농업생물부 농업미생물과) ;
  • 이상엽 (농업생물부 농업미생물과) ;
  • 이승돈 (농촌진흥청 연구정책국 연구조정과)
  • Received : 2010.05.03
  • Accepted : 2010.05.18
  • Published : 2010.08.01

Abstract

To screen for potential biocontrol agents against postharvest disease of garlics caused by Penicillium hirsutum, a total of 1292 isolates were isolated from the rhizoshere or rhizoplane of Allium species. Among them, S59-4 isolate was selected as a potential biocontrol agent by in vivo wounded garlic bulb assay. The isolate was identified as Pantoea agglomerans (Pa59-4) through Biolog system. Pa59-4 did not inhibit the mycelial growth of P. hirsutum in dual-culture with P. hirsutum on tryptic soy agar. In order to elucidate mode of action of Pa59-4 on biological control, nutrient competition between Pa59-4 and P. hirsutum was investigated by the simple method using tissue culture plates with cylinder inserts containing defusing membrane reported by Janisiewicz et al. (2000). The results showed that Pa59-4 effectively suppressed spore germination and mycelial growth of blue mold in the low concentration (0.5%) of garlic juice, but it did not suppress those of blue mold in the high concentration (5%) of garlic juice. This result suggests that the mechanism in biocontrol of garlic blue mold by Pa 59-4 may be involved in nutrient competition with P. hirsutum on garlic bulbs.

마늘 저장병을 일으키는 푸른곰팡이병을 생물적으로 방제하기 위하여 마늘과 양파 등 파속 채소 근권 또는 근면으로부터 미생물 1,292균주를 분리하였다. 분리한 균주를 대상으로 푸른곰팡이병에 대한 항균성 검정과 마늘인 편검정을 실시하여 최종적으로 푸른곰팡이병 진전을 현저히 억제하는 S59-4균주를 선발하였으며, Biolog system을 이용하여 동정한 결과 Pantoea agglomerans 59-4(Pa59-4)로 동정되었다. 선발한 길항균 Pa59-4의 억제기작을 조사하기 위하여 TSA배지 상에서 병원균과 대치 배양하였을 때 항균활성을 보이지 않았다. 길항균 Pa59-4과 병원균간의 영양원 경합여부를 Janisiewicz(2000)의 방법으로 조사한 결과, 저농도로 마늘즙액에 병원균과 길항균 Pa59-4를 함께 처리하였을 때, 길항균 Pa59-4는 병원균의 포자발아 및 균사생장을 현저히 억제하였으며, 길항균 Pa59-4와 함께 접종하였을 때 발아되지 않았던 병원균 포자를 신선한 5% 마늘쥬스에 접종했을 때에는 다시 잘 발아 되는 것으로 나타나 길항균 Pa59-4균주의 억제기작은 영양원 경합에 의한 것으로 확인되었다. 이상의 결과로 볼 때, 길항균 Pa59-4는 마늘저장 중 부패를 줄일 수 있는 유망한 길항균으로 판단되었다.

Keywords

References

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