Journal of Veterinary Clinics (한국임상수의학회지)

The Korean Society of Veterinary Clinics (한국임상수의학회)
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Occurrence of Atypical Aeromonas Salmonicida in Mandarin Fish (SSOGARI: Korean Name) Siniperca Scherzeri

양식 쏘가리에서 Atypical Aeromonas Salmonicida의 발병

  • Park, Seong-Bin (Aquatic Biotechnology Center of WCU Project, College of Veterinary Medicine, Gyeongsang National University) ;
  • Nho, Seong-Won (Aquatic Biotechnology Center of WCU Project, College of Veterinary Medicine, Gyeongsang National University) ;
  • Jang, Ho-Bin (Aquatic Biotechnology Center of WCU Project, College of Veterinary Medicine, Gyeongsang National University) ;
  • Cha, In-Seok (Aquatic Biotechnology Center of WCU Project, College of Veterinary Medicine, Gyeongsang National University) ;
  • Kim, Young-Rim (Aquatic Biotechnology Center of WCU Project, College of Veterinary Medicine, Gyeongsang National University) ;
  • Ha, Mi-Ae (Aquatic Biotechnology Center of WCU Project, College of Veterinary Medicine, Gyeongsang National University) ;
  • Kang, Sang-Chul (College of Veterinary Medicine and Veterinary Medical Research Institute, Jeju National University) ;
  • Kim, Jae-Hoon (College of Veterinary Medicine and Veterinary Medical Research Institute, Jeju National University) ;
  • Jung, Tae-Sung (Aquatic Biotechnology Center of WCU Project, College of Veterinary Medicine, Gyeongsang National University)
  • Accepted : 2010.05.25
  • Published : 2010.06.30
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Abstract

Mandarin fish (SSOGARI: Korean name) has emerged as new aquaculture and aquarium fish among Korean native fish species. Unfortunately, this fish could not exempt from infectious agent invasion. As an epidemiological survey, high mortality of mandarin fish (Siniperca scherzeri) observed from a fish farm in Gyeongnam province of South Korea. The major macroscopic sign of the diseased fish was severe multifocal dermal ulceration. Histological observation revealed inflammation, necrosis and colonization of bacteria in various tissues. Seven bacterial isolates from the diseased fish were identified as Aeromonas salmonicida subsp. salmonicida (typical A. salmonicida) using API 20E and 20NE system. However, the polymerase chain reaction (PCR) assay using two different primers (AP and MIY) revealed that the bacterial strains were not typical A. salmonicida, but atypical A. salmonicida. This study demonstrates that PCR assay is a reliable method for the confirmation of atypical A. salmonicida in mandarin fish as compared to biochemical tests. Furthermore, this is the first account of extensive dermatitis in mandarin fish due to atypical A. salmonicida infection, which has high potential in aquaculture and aquarium fish among native fish species.

경남 산청지방에서 쏘가리를 양식하던 어장에서 대량폐사가 발생하였다. 주요 증상으로 전신 피부에서 궤양이 진행되었고 조직학적으로는 주요 실질 장기에서 세균의 집락과 주변부위로 염증 및 괴사가 관찰되었다. 분리된 세균을 API 20E 및 API 20NE를 이용하여 생화학적 검사를 실시한 결과 Aeromonas salmonicida subsp. salmonicida (typical A. salmonicida)로 진단할 수 있었다. 그러나 atypical A. salmonicida와 typical A. salmonicida로 구분할 수 있는 PCR 기법을 실시한 결과, 본 세균은 atypical A. salmonicida임을 확인할 수 있었다. 본 연구는 우리나라 쏘가리에서 A. salmonicida로 인한 발병의 최초 보고임과 동시에, A. salmonicida의 정확한 진단을 위해선 생화학적 검사가 아닌 PCR 검사를 이용한 진단을 실시해야 함을 제시하였다.

Keywords

References

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